BRENDA - Enzyme Database show
show all sequences of 1.1.1.217

Purification and properties of an asymmetric reduction enzyme of 2-methyl-3-oxobutyrate in baker's yeast

Furuichi, A.; Akita, H.; Matsukura, H.; Oishi, T.; Horikoshi, K.; Agric. Biol. Chem. 49, 2563-2570 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
FMN
-
Saccharomyces cerevisiae
KCN
-
Saccharomyces cerevisiae
NADH
-
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.42
-
Benzyl 2-methyl-3-oxobutyrate
-
Saccharomyces cerevisiae
4.2
-
benzyl (2R,3S)-2-methyl-3-hydroxy-butanoate
-
Saccharomyces cerevisiae
4.2
-
benzyl(2S,3S)-2-methyl-3-hydroxybutanoate
-
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ag+
strong inhibition
Saccharomyces cerevisiae
Cu2+
strong inhibition
Saccharomyces cerevisiae
Hg2+
strong inhibition
Saccharomyces cerevisiae
Ni2+
strong inhibition
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
SDS-PAGE
Saccharomyces cerevisiae
32000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
Saccharomyces cerevisiae
-
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
Saccharomyces cerevisiae
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0063
-
crude extract
Saccharomyces cerevisiae
0.9
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
286280
Saccharomyces cerevisiae
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
286280
Saccharomyces cerevisiae
r
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
286280
Saccharomyces cerevisiae
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
ratio syn-(2) and anti-(3) conformation 8:1 for purified enzyme
286280
Saccharomyces cerevisiae
r
additional information
no reduction of aldehydes or alcohols
286280
Saccharomyces cerevisiae
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
-
Saccharomyces cerevisiae
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Saccharomyces cerevisiae
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
60
-
Saccharomyces cerevisiae
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
rapid loss of activity above 40C
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8
less than 10% activity at pH 4 and 9
Saccharomyces cerevisiae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
purified enzyme at pH 6-8, rapid loss of activity below pH 6 and above pH 9
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
FMN
-
Saccharomyces cerevisiae
KCN
-
Saccharomyces cerevisiae
NADH
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.42
-
Benzyl 2-methyl-3-oxobutyrate
-
Saccharomyces cerevisiae
4.2
-
benzyl (2R,3S)-2-methyl-3-hydroxy-butanoate
-
Saccharomyces cerevisiae
4.2
-
benzyl(2S,3S)-2-methyl-3-hydroxybutanoate
-
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ag+
strong inhibition
Saccharomyces cerevisiae
Cu2+
strong inhibition
Saccharomyces cerevisiae
Hg2+
strong inhibition
Saccharomyces cerevisiae
Ni2+
strong inhibition
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
SDS-PAGE
Saccharomyces cerevisiae
32000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
Saccharomyces cerevisiae
-
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
Saccharomyces cerevisiae
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0063
-
crude extract
Saccharomyces cerevisiae
0.9
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
286280
Saccharomyces cerevisiae
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
286280
Saccharomyces cerevisiae
r
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
286280
Saccharomyces cerevisiae
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
ratio syn-(2) and anti-(3) conformation 8:1 for purified enzyme
286280
Saccharomyces cerevisiae
r
additional information
no reduction of aldehydes or alcohols
286280
Saccharomyces cerevisiae
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Saccharomyces cerevisiae
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Saccharomyces cerevisiae
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
60
-
Saccharomyces cerevisiae
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
rapid loss of activity above 40C
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8
less than 10% activity at pH 4 and 9
Saccharomyces cerevisiae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
purified enzyme at pH 6-8, rapid loss of activity below pH 6 and above pH 9
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.217
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286280
Furuichi
-
Purification and properties of ...
Saccharomyces cerevisiae
Agric. Biol. Chem.
49
2563-2570
1985
-
-
-
-
-
-
3
3
-
4
2
1
-
1
-
-
1
-
-
-
2
-
3
1
1
1
1
-
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
3
-
3
-
4
2
1
-
-
-
1
-
-
2
-
3
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-