BRENDA - Enzyme Database show
show all sequences of 1.1.1.216

NADP+-dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

Mayoral, J.G.; Nouzova, M.; Navare, A.; Noriega, F.G.; Proc. Natl. Acad. Sci. USA 106, 21091-21096 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Aedes aegypti
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.091
-
(2E,6E)-farnesol
pH 10.0, 30C
Aedes aegypti
0.097
-
(2Z,6Z)-farnesol
pH 10.0, 30C
Aedes aegypti
0.109
-
(Z)-nerol
pH 10.0, 30C
Aedes aegypti
0.133
-
citronellol
pH 10.0, 30C
Aedes aegypti
0.184
-
2-decanol
pH 10.0, 30C
Aedes aegypti
0.195
-
Octanol
pH 10.0, 30C
Aedes aegypti
0.208
-
(E)-geraniol
pH 10.0, 30C
Aedes aegypti
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
2 * 29000, calculated
Aedes aegypti
60000
-
gel filtration
Aedes aegypti
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aedes aegypti
D2WKD9
NADP+-dependent farnesol dehydrogenase 1
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Aedes aegypti
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
and midgut, of adult female, highest levels of mRNA
Aedes aegypti
-
corpora allata
no farnesol dehydrogenase activity in newly emerged mosquitoes but significant activity 24 h later. SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
Aedes aegypti
-
fat body
and Malpighian tubules, considerable levles of mRNA
Aedes aegypti
-
ganglion
abdominal and thoracic ganglia, high level of mRNA
Aedes aegypti
-
malpighian tubule
and fat body, considerable levles of mRNA
Aedes aegypti
-
midgut
and brain, of adult female, highest levels of mRNA
Aedes aegypti
-
testis
of adult male, substantial level of mRNA
Aedes aegypti
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
9
-
substrate (2Z,6Z)-farnesol, pH 10.0, 30C
Aedes aegypti
9.7
-
substrate (2E,6E)-farnesol, pH 10.0, 30C
Aedes aegypti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesol + NADP+
enzyme shows highest affinity for farnesol
713402
Aedes aegypti
(2E,6E)-farnesal + NADPH + H+
-
-
-
i
(2Z,6Z)-farnesol + NADP+
enzyme shows highest affinity for farnesol
713402
Aedes aegypti
(2Z,6Z)-farnesal + NADPH + H+
-
-
-
r
(E)-geraniol + NADP+
-
713402
Aedes aegypti
(E)-geranial + NADPH + H+
-
-
-
?
(Z)-nerol + NADP+
-
713402
Aedes aegypti
(Z)-neral + NADPH + H+
-
-
-
?
2-decanol + NADP+
-
713402
Aedes aegypti
? + NADPH + H+
-
-
-
?
citronellol + NADP+
-
713402
Aedes aegypti
citronellal + NADPH + H+
-
-
-
?
additional information
no activity is detected for butanol, ethanol, or glycerol, or for farnesal or farnesoic acid
713402
Aedes aegypti
?
-
-
-
-
octanol + NADP+
-
713402
Aedes aegypti
octanal + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 29000, calculated
Aedes aegypti
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
-
Aedes aegypti
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
absolutely required, NAD+ or FAD cannot substitute
Aedes aegypti
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Aedes aegypti
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
absolutely required, NAD+ or FAD cannot substitute
Aedes aegypti
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.091
-
(2E,6E)-farnesol
pH 10.0, 30C
Aedes aegypti
0.097
-
(2Z,6Z)-farnesol
pH 10.0, 30C
Aedes aegypti
0.109
-
(Z)-nerol
pH 10.0, 30C
Aedes aegypti
0.133
-
citronellol
pH 10.0, 30C
Aedes aegypti
0.184
-
2-decanol
pH 10.0, 30C
Aedes aegypti
0.195
-
Octanol
pH 10.0, 30C
Aedes aegypti
0.208
-
(E)-geraniol
pH 10.0, 30C
Aedes aegypti
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
2 * 29000, calculated
Aedes aegypti
60000
-
gel filtration
Aedes aegypti
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Aedes aegypti
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
and midgut, of adult female, highest levels of mRNA
Aedes aegypti
-
corpora allata
no farnesol dehydrogenase activity in newly emerged mosquitoes but significant activity 24 h later. SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
Aedes aegypti
-
fat body
and Malpighian tubules, considerable levles of mRNA
Aedes aegypti
-
ganglion
abdominal and thoracic ganglia, high level of mRNA
Aedes aegypti
-
malpighian tubule
and fat body, considerable levles of mRNA
Aedes aegypti
-
midgut
and brain, of adult female, highest levels of mRNA
Aedes aegypti
-
testis
of adult male, substantial level of mRNA
Aedes aegypti
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
9
-
substrate (2Z,6Z)-farnesol, pH 10.0, 30C
Aedes aegypti
9.7
-
substrate (2E,6E)-farnesol, pH 10.0, 30C
Aedes aegypti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesol + NADP+
enzyme shows highest affinity for farnesol
713402
Aedes aegypti
(2E,6E)-farnesal + NADPH + H+
-
-
-
i
(2Z,6Z)-farnesol + NADP+
enzyme shows highest affinity for farnesol
713402
Aedes aegypti
(2Z,6Z)-farnesal + NADPH + H+
-
-
-
r
(E)-geraniol + NADP+
-
713402
Aedes aegypti
(E)-geranial + NADPH + H+
-
-
-
?
(Z)-nerol + NADP+
-
713402
Aedes aegypti
(Z)-neral + NADPH + H+
-
-
-
?
2-decanol + NADP+
-
713402
Aedes aegypti
? + NADPH + H+
-
-
-
?
citronellol + NADP+
-
713402
Aedes aegypti
citronellal + NADPH + H+
-
-
-
?
additional information
no activity is detected for butanol, ethanol, or glycerol, or for farnesal or farnesoic acid
713402
Aedes aegypti
?
-
-
-
-
octanol + NADP+
-
713402
Aedes aegypti
octanal + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 29000, calculated
Aedes aegypti
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
-
Aedes aegypti
Expression
Organism
Commentary
Expression
Aedes aegypti
SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
additional information
Expression (protein specific)
Organism
Commentary
Expression
Aedes aegypti
SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
additional information
Other publictions for EC 1.1.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713402
Mayoral
NADP+-dependent farnesol dehyd ...
Aedes aegypti
Proc. Natl. Acad. Sci. USA
106
21091-21096
2009
-
-
1
-
-
-
-
7
-
-
2
-
-
1
-
-
1
-
-
7
2
-
8
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
7
-
-
2
-
-
-
-
1
-
7
2
-
8
1
-
-
-
-
1
-
-
-
1
-
-
1
-
-
347994
Sperry
Farnesol oxidation in insects: ...
Manduca sexta
Insect Biochem. Mol. Biol.
31
171-178
2001
1
-
-
-
-
-
4
1
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
347993
Inoue
Characterization and activity ...
Ipomoea batatas
Agric. Biol. Chem.
48
733-738
1984
-
-
-
-
-
-
6
2
-
-
2
-
-
1
-
-
1
-
-
1
-
-
6
1
1
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
2
-
-
2
-
-
-
-
1
-
1
-
-
6
1
1
-
-
-
2
2
-
-
-
-
-
-
-
-