BRENDA - Enzyme Database show
show all sequences of 1.1.1.216

Characterization and activity change of farnesol dehydrogenase in black rot fungus-infected sweet potato.

Inoue, H.; Tsuji, H.; Uritani, I.; Agric. Biol. Chem. 48, 733-738 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuCl2
-
Ipomoea batatas
EDTA
-
Ipomoea batatas
Monoiodoacetamide
-
Ipomoea batatas
additional information
inhibitory effect of thiol reagents indicates that the SH-group of the enzyme participates in its activity
Ipomoea batatas
N-ethylmaleimide
-
Ipomoea batatas
ZnCl2
-
Ipomoea batatas
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0156
-
trans,trans-farnesol
-
Ipomoea batatas
0.729
-
geraniol
-
Ipomoea batatas
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
2 * 47000, SDS-PAGE
Ipomoea batatas
90000
-
gel filtation
Ipomoea batatas
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ipomoea batatas
-
sweet potato, enzyme is formed in response to infection by the black rot fungus Ceratocystis fimbriata
-
Purification (Commentary)
Commentary
Organism
-
Ipomoea batatas
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Ipomoea batatas
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cis,trans-farnesol + NADP+
-
347993
Ipomoea batatas
cis,trans-farnesal + NADPH
-
347993
Ipomoea batatas
?
citronerol + NADP+
-
347993
Ipomoea batatas
?
-
-
-
?
decanol + NADP+
-
347993
Ipomoea batatas
decanal + NADPH
-
-
-
?
geraniol + NADP+
-
347993
Ipomoea batatas
?
-
-
-
?
nerol + NADP+
-
347993
Ipomoea batatas
?
-
-
-
?
trans,trans-farnesol + NADP+
preferred substrate
347993
Ipomoea batatas
trans,trans-farnesal + NADPH
-
347993
Ipomoea batatas
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 47000, SDS-PAGE
Ipomoea batatas
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Ipomoea batatas
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
reduction of trans,trans-farnesal
Ipomoea batatas
9.5
-
oxidation of trans,trans-farnesol
Ipomoea batatas
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
reduction of trans,trans-farnesal: 60% of maximal activity at pH 6.5 and at pH 8
Ipomoea batatas
7
10.5
oxidation of trans,trans-farnesol: 10% of maximal activity at pH 7.0, 30% of maximal activity at pH 10.2
Ipomoea batatas
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
NAD+ cannot replace NADP+
Ipomoea batatas
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
NAD+ cannot replace NADP+
Ipomoea batatas
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuCl2
-
Ipomoea batatas
EDTA
-
Ipomoea batatas
Monoiodoacetamide
-
Ipomoea batatas
additional information
inhibitory effect of thiol reagents indicates that the SH-group of the enzyme participates in its activity
Ipomoea batatas
N-ethylmaleimide
-
Ipomoea batatas
ZnCl2
-
Ipomoea batatas
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0156
-
trans,trans-farnesol
-
Ipomoea batatas
0.729
-
geraniol
-
Ipomoea batatas
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
2 * 47000, SDS-PAGE
Ipomoea batatas
90000
-
gel filtation
Ipomoea batatas
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ipomoea batatas
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Ipomoea batatas
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cis,trans-farnesol + NADP+
-
347993
Ipomoea batatas
cis,trans-farnesal + NADPH
-
347993
Ipomoea batatas
?
citronerol + NADP+
-
347993
Ipomoea batatas
?
-
-
-
?
decanol + NADP+
-
347993
Ipomoea batatas
decanal + NADPH
-
-
-
?
geraniol + NADP+
-
347993
Ipomoea batatas
?
-
-
-
?
nerol + NADP+
-
347993
Ipomoea batatas
?
-
-
-
?
trans,trans-farnesol + NADP+
preferred substrate
347993
Ipomoea batatas
trans,trans-farnesal + NADPH
-
347993
Ipomoea batatas
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 47000, SDS-PAGE
Ipomoea batatas
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Ipomoea batatas
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
reduction of trans,trans-farnesal
Ipomoea batatas
9.5
-
oxidation of trans,trans-farnesol
Ipomoea batatas
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
reduction of trans,trans-farnesal: 60% of maximal activity at pH 6.5 and at pH 8
Ipomoea batatas
7
10.5
oxidation of trans,trans-farnesol: 10% of maximal activity at pH 7.0, 30% of maximal activity at pH 10.2
Ipomoea batatas
Other publictions for EC 1.1.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713402
Mayoral
NADP+-dependent farnesol dehyd ...
Aedes aegypti
Proc. Natl. Acad. Sci. USA
106
21091-21096
2009
-
-
1
-
-
-
-
7
-
-
2
-
-
1
-
-
1
-
-
7
2
-
8
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
7
-
-
2
-
-
-
-
1
-
7
2
-
8
1
-
-
-
-
1
-
-
-
1
-
-
1
-
-
347994
Sperry
Farnesol oxidation in insects: ...
Manduca sexta
Insect Biochem. Mol. Biol.
31
171-178
2001
1
-
-
-
-
-
4
1
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
347993
Inoue
Characterization and activity ...
Ipomoea batatas
Agric. Biol. Chem.
48
733-738
1984
-
-
-
-
-
-
6
2
-
-
2
-
-
1
-
-
1
-
-
1
-
-
6
1
1
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
2
-
-
2
-
-
-
-
1
-
1
-
-
6
1
1
-
-
-
2
2
-
-
-
-
-
-
-
-