BRENDA - Enzyme Database show
show all sequences of 1.1.1.215

A temperature-regulated Campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization

Pajaniappan, M.; Hall, J.E.; Cawthraw, S.A.; Newell, D.G.; Gaynor, E.C.; Fields, J.A.; Rathbun, K.M.; Agee, W.A.; Burns, C.M.; Hall, S.J.; Kelly, D.J.; Thompson, S.A.; Mol. Microbiol. 68, 474-491 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
expression and activity of cj0414 and cj0415 are higher at 42C than at 37C
Campylobacter jejuni
Application
Application
Commentary
Organism
medicine
ability of Campylobacter jejuni to use gluconate as an electron donor via GADH activity is an important metabolic characteristic that is required for full colonization of avian but not mammalian hosts
Campylobacter jejuni
Cloned(Commentary)
Commentary
Organism
mutagenized cj0414 and cj0415 alleles are introduced into Campylobacter jejuni 81-176
Campylobacter jejuni
Engineering
Amino acid exchange
Commentary
Organism
additional information
cj0414 and cj0415 mutants lack GADH activity at either temperature. Cj0415 mutant does not exhibit gluconate-dependent respiration. Cj0415 mutant is defective in chicken colonization, but exhibits only a minor colonization defect in mice
Campylobacter jejuni
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Campylobacter jejuni
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26900
-
sequence analysis, cj0414
Campylobacter jejuni
63700
-
sequence analysis, cj0415
Campylobacter jejuni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Campylobacter jejuni
-
strain 81-176
-
Campylobacter jejuni 81-176
-
strain 81-176
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-gluconate + NADP+
wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization
700265
Campylobacter jejuni
2-dehydro-D-gluconate + NADPH + H+
-
-
-
?
D-gluconate + NADP+
wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization
700265
Campylobacter jejuni 81-176
2-dehydro-D-gluconate + NADPH + H+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Campylobacter jejuni
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Campylobacter jejuni
cj0414, isoelectric focusing
-
5.5
Campylobacter jejuni
cj0415, isoelectric focusing
-
8.8
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
expression and activity of cj0414 and cj0415 are higher at 42C than at 37C
Campylobacter jejuni
Application (protein specific)
Application
Commentary
Organism
medicine
ability of Campylobacter jejuni to use gluconate as an electron donor via GADH activity is an important metabolic characteristic that is required for full colonization of avian but not mammalian hosts
Campylobacter jejuni
Cloned(Commentary) (protein specific)
Commentary
Organism
mutagenized cj0414 and cj0415 alleles are introduced into Campylobacter jejuni 81-176
Campylobacter jejuni
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Campylobacter jejuni
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
cj0414 and cj0415 mutants lack GADH activity at either temperature. Cj0415 mutant does not exhibit gluconate-dependent respiration. Cj0415 mutant is defective in chicken colonization, but exhibits only a minor colonization defect in mice
Campylobacter jejuni
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Campylobacter jejuni
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26900
-
sequence analysis, cj0414
Campylobacter jejuni
63700
-
sequence analysis, cj0415
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-gluconate + NADP+
wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization
700265
Campylobacter jejuni
2-dehydro-D-gluconate + NADPH + H+
-
-
-
?
D-gluconate + NADP+
wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization
700265
Campylobacter jejuni 81-176
2-dehydro-D-gluconate + NADPH + H+
-
-
-
?
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Campylobacter jejuni
cj0414, isoelectric focusing
-
5.5
Campylobacter jejuni
cj0415, isoelectric focusing
-
8.8
Other publictions for EC 1.1.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721369
Rauch
Characterization of enzymes in ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Appl. Microbiol. Biotechnol.
88
711-718
2010
-
-
1
-
-
-
-
3
-
-
2
4
-
6
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
2
4
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
695779
De Werra
Role of gluconic acid producti ...
Pseudomonas fluorescens, Pseudomonas fluorescens CHA0
Appl. Environ. Microbiol.
75
4162-4174
2009
-
-
1
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700265
Pajaniappan
A temperature-regulated Campyl ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Mol. Microbiol.
68
474-491
2008
1
1
1
-
1
-
-
-
1
-
2
-
-
13
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
1
1
1
1
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
684567
Toyama
Membrane-bound, 2-keto-D-gluco ...
Gluconobacter frateurii, Gluconobacter frateurii IFO 3271
Appl. Environ. Microbiol.
73
6551-6556
2007
-
-
1
-
-
-
-
-
1
1
3
2
-
3
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
3
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685666
Saichana
Preparation of enzymes require ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Biosci. Biotechnol. Biochem.
71
2478-2486
2007
-
-
1
-
-
-
-
-
1
1
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
4
-
3
-
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286279
Yum
The yiaE gene, located at 80.1 ...
Escherichia coli
J. Bacteriol.
180
5984-5988
1998
-
-
1
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5693
Pitt
Enzymes of gluconate metabolis ...
Penicillium chrysogenum, Penicillium chrysogenum Westling
Antonie van Leeuwenhoek
51
353-364
1985
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286275
Ameyama
-
2-keto-D-Gluconate reductase f ...
Acetobacter ascendens, Acetobacter pasteurianus, Gluconacetobacter liquefaciens, Gluconobacter oxydans
Methods Enzymol.
89
203-210
1982
-
-
-
3
-
-
8
-
4
4
5
3
-
4
-
-
3
-
-
-
3
4
31
2
-
-
4
-
8
-
4
12
-
-
-
-
-
-
12
3
-
-
-
8
-
-
4
4
5
3
-
-
-
3
-
-
3
4
31
2
-
-
4
-
8
-
4
-
-
-
-
-
-
-
286274
Adachi
Crystalline 2-ketogluconate re ...
Acetobacter ascendens
Agric. Biol. Chem.
42
2057-2062
1978
-
-
-
1
-
3
1
4
1
1
2
-
-
1
-
-
1
-
-
-
1
1
7
1
1
1
3
-
2
2
-
2
-
-
-
-
-
-
2
1
-
3
-
1
-
4
1
1
2
-
-
-
-
1
-
-
1
1
7
1
1
1
3
-
2
2
-
-
-
-
-
-
-
-
286276
Shinagawa
-
Distribution of gluconate dehy ...
acetic acid bacteria, no activity in a number of oxidative or aerobic bacteria
Agric. Biol. Chem.
42
1055-1057
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286273
Chiyonobu
-
Purification, crystallization ...
Acetobacter pasteurianus
Agric. Biol. Chem.
40
175-184
1976
-
-
-
1
-
2
9
12
1
1
2
1
-
1
-
-
1
-
-
-
1
2
10
1
1
1
2
-
2
2
1
2
-
-
-
-
-
-
2
1
-
2
-
9
-
12
1
1
2
1
-
-
-
1
-
-
1
2
10
1
1
1
2
-
2
2
1
-
-
-
-
-
-
-