BRENDA - Enzyme Database show
show all sequences of 1.1.1.215

Membrane-bound, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase from Gluconobacter dioxyacetonicus IFO 3271: molecular properties and gene disruption

Toyama, H.; Furuya, N.; Saichana, I.; Ano, Y.; Adachi, O.; Matsushita, K.; Appl. Environ. Microbiol. 73, 6551-6556 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits
Gluconobacter frateurii
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Gluconobacter frateurii
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
heme group of cytochrome C bound to the middle subunit
Gluconobacter frateurii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
44000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
65000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-gluconate + NADP+
Gluconobacter frateurii
-
2-dehydro-D-gluconate + NADPH
-
-
?
D-gluconate + NADP+
Gluconobacter frateurii IFO 3271
-
2-dehydro-D-gluconate + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter frateurii
A4PIB0
the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; strain IFO 3271, gene gndL encoding the large subunit of the enzyme
-
Gluconobacter frateurii IFO 3271
A4PIB0
the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; strain IFO 3271, gene gndL encoding the large subunit of the enzyme
-
Purification (Commentary)
Commentary
Organism
native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography
Gluconobacter frateurii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
137
-
purified native enzyme
Gluconobacter frateurii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-gluconate + NADP+
-
684567
Gluconobacter frateurii
2-dehydro-D-gluconate + NADPH
-
-
-
?
D-gluconate + NADP+
the large subunit is the catalytically active enzyme part, high substrate and regiospecificity
684567
Gluconobacter frateurii
2-dehydro-D-gluconate + NADPH
-
-
-
?
D-gluconate + NADP+
-
684567
Gluconobacter frateurii IFO 3271
2-dehydro-D-gluconate + NADPH
-
-
-
?
D-gluconate + NADP+
the large subunit is the catalytically active enzyme part, high substrate and regiospecificity
684567
Gluconobacter frateurii IFO 3271
2-dehydro-D-gluconate + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
trimer
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter frateurii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Gluconobacter frateurii
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c
bound by the 44 kDa subunit
Gluconobacter frateurii
FAD
bound to the enzyme's large 65 kDA subunit
Gluconobacter frateurii
NADP+
-
Gluconobacter frateurii
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits
Gluconobacter frateurii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c
bound by the 44 kDa subunit
Gluconobacter frateurii
FAD
bound to the enzyme's large 65 kDA subunit
Gluconobacter frateurii
NADP+
-
Gluconobacter frateurii
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Gluconobacter frateurii
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
heme group of cytochrome C bound to the middle subunit
Gluconobacter frateurii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
44000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
65000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-gluconate + NADP+
Gluconobacter frateurii
-
2-dehydro-D-gluconate + NADPH
-
-
?
D-gluconate + NADP+
Gluconobacter frateurii IFO 3271
-
2-dehydro-D-gluconate + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography
Gluconobacter frateurii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
137
-
purified native enzyme
Gluconobacter frateurii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-gluconate + NADP+
-
684567
Gluconobacter frateurii
2-dehydro-D-gluconate + NADPH
-
-
-
?
D-gluconate + NADP+
the large subunit is the catalytically active enzyme part, high substrate and regiospecificity
684567
Gluconobacter frateurii
2-dehydro-D-gluconate + NADPH
-
-
-
?
D-gluconate + NADP+
-
684567
Gluconobacter frateurii IFO 3271
2-dehydro-D-gluconate + NADPH
-
-
-
?
D-gluconate + NADP+
the large subunit is the catalytically active enzyme part, high substrate and regiospecificity
684567
Gluconobacter frateurii IFO 3271
2-dehydro-D-gluconate + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE
Gluconobacter frateurii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter frateurii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Gluconobacter frateurii
Other publictions for EC 1.1.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721369
Rauch
Characterization of enzymes in ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Appl. Microbiol. Biotechnol.
88
711-718
2010
-
-
1
-
-
-
-
3
-
-
2
4
-
6
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
2
4
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
695779
De Werra
Role of gluconic acid producti ...
Pseudomonas fluorescens, Pseudomonas fluorescens CHA0
Appl. Environ. Microbiol.
75
4162-4174
2009
-
-
1
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700265
Pajaniappan
A temperature-regulated Campyl ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Mol. Microbiol.
68
474-491
2008
1
1
1
-
1
-
-
-
1
-
2
-
-
13
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
1
1
1
1
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
684567
Toyama
Membrane-bound, 2-keto-D-gluco ...
Gluconobacter frateurii, Gluconobacter frateurii IFO 3271
Appl. Environ. Microbiol.
73
6551-6556
2007
-
-
1
-
-
-
-
-
1
1
3
2
-
3
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
3
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685666
Saichana
Preparation of enzymes require ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Biosci. Biotechnol. Biochem.
71
2478-2486
2007
-
-
1
-
-
-
-
-
1
1
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
4
-
3
-
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286279
Yum
The yiaE gene, located at 80.1 ...
Escherichia coli
J. Bacteriol.
180
5984-5988
1998
-
-
1
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5693
Pitt
Enzymes of gluconate metabolis ...
Penicillium chrysogenum, Penicillium chrysogenum Westling
Antonie van Leeuwenhoek
51
353-364
1985
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286275
Ameyama
-
2-keto-D-Gluconate reductase f ...
Acetobacter ascendens, Acetobacter pasteurianus, Gluconacetobacter liquefaciens, Gluconobacter oxydans
Methods Enzymol.
89
203-210
1982
-
-
-
3
-
-
8
-
4
4
5
3
-
4
-
-
3
-
-
-
3
4
31
2
-
-
4
-
8
-
4
12
-
-
-
-
-
-
12
3
-
-
-
8
-
-
4
4
5
3
-
-
-
3
-
-
3
4
31
2
-
-
4
-
8
-
4
-
-
-
-
-
-
-
286274
Adachi
Crystalline 2-ketogluconate re ...
Acetobacter ascendens
Agric. Biol. Chem.
42
2057-2062
1978
-
-
-
1
-
3
1
4
1
1
2
-
-
1
-
-
1
-
-
-
1
1
7
1
1
1
3
-
2
2
-
2
-
-
-
-
-
-
2
1
-
3
-
1
-
4
1
1
2
-
-
-
-
1
-
-
1
1
7
1
1
1
3
-
2
2
-
-
-
-
-
-
-
-
286276
Shinagawa
-
Distribution of gluconate dehy ...
acetic acid bacteria, no activity in a number of oxidative or aerobic bacteria
Agric. Biol. Chem.
42
1055-1057
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286273
Chiyonobu
-
Purification, crystallization ...
Acetobacter pasteurianus
Agric. Biol. Chem.
40
175-184
1976
-
-
-
1
-
2
9
12
1
1
2
1
-
1
-
-
1
-
-
-
1
2
10
1
1
1
2
-
2
2
1
2
-
-
-
-
-
-
2
1
-
2
-
9
-
12
1
1
2
1
-
-
-
1
-
-
1
2
10
1
1
1
2
-
2
2
1
-
-
-
-
-
-
-