BRENDA - Enzyme Database show
show all sequences of 1.1.1.215

Purification and characterization of the 2-ketoaldonate reductase from Brevibacterium ketosoreductum ATCC21914

Yum, D-Y.; Bae, S-S.; Pan, J-G.; Biosci. Biotechnol. Biochem. 62, 154-156 (1998)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
-
Brevibacterium ketosoreductum
5
-
2,5-diketo-D-gluconate
-
Brevibacterium ketosoreductum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
72000
-
gel filtration
Brevibacterium ketosoreductum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,5-diketo-D-gluconate + NADPH
Brevibacterium ketosoreductum
-
5-keto-D-gluconate + NADP+
-
Brevibacterium ketosoreductum
ir
2,5-diketo-D-gluconate + NADPH
Brevibacterium ketosoreductum ATCC21914
-
5-keto-D-gluconate + NADP+
-
Brevibacterium ketosoreductum ATCC21914
ir
2-dehydro-D-gluconate + NADPH
Brevibacterium ketosoreductum
-
D-gluconate + NADP+
-
Brevibacterium ketosoreductum
-
2-dehydro-D-gluconate + NADPH
Brevibacterium ketosoreductum ATCC21914
-
D-gluconate + NADP+
-
Brevibacterium ketosoreductum ATCC21914
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brevibacterium ketosoreductum
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200
Brevibacterium ketosoreductum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.2
-
-
Brevibacterium ketosoreductum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5-diketo-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum
5-keto-D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum
ir
2,5-diketo-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum ATCC21914
5-keto-D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
ir
2-dehydro-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum
-
2-dehydro-D-gluconate + NADPH
11.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum
?
2-dehydro-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum ATCC21914
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
-
2-dehydro-D-gluconate + NADPH
11.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum ATCC21914
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
?
2-keto-L-gulonate + NADPH
10.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum
L-idonate + NADP+
-
286278
Brevibacterium ketosoreductum
?
2-keto-L-gulonate + NADPH
10.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum ATCC21914
L-idonate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Brevibacterium ketosoreductum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
35% of activity left after incubation at pH 8.4 for 2 h at 30°C, no remaining activity below pH 5.2 or above pH 10.0
Brevibacterium ketosoreductum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Brevibacterium ketosoreductum
NADPH
-
Brevibacterium ketosoreductum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Brevibacterium ketosoreductum
NADPH
-
Brevibacterium ketosoreductum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
-
Brevibacterium ketosoreductum
5
-
2,5-diketo-D-gluconate
-
Brevibacterium ketosoreductum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
72000
-
gel filtration
Brevibacterium ketosoreductum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,5-diketo-D-gluconate + NADPH
Brevibacterium ketosoreductum
-
5-keto-D-gluconate + NADP+
-
Brevibacterium ketosoreductum
ir
2,5-diketo-D-gluconate + NADPH
Brevibacterium ketosoreductum ATCC21914
-
5-keto-D-gluconate + NADP+
-
Brevibacterium ketosoreductum ATCC21914
ir
2-dehydro-D-gluconate + NADPH
Brevibacterium ketosoreductum
-
D-gluconate + NADP+
-
Brevibacterium ketosoreductum
-
2-dehydro-D-gluconate + NADPH
Brevibacterium ketosoreductum ATCC21914
-
D-gluconate + NADP+
-
Brevibacterium ketosoreductum ATCC21914
-
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200
Brevibacterium ketosoreductum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.2
-
-
Brevibacterium ketosoreductum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5-diketo-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum
5-keto-D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum
ir
2,5-diketo-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum ATCC21914
5-keto-D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
ir
2-dehydro-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum
-
2-dehydro-D-gluconate + NADPH
11.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum
?
2-dehydro-D-gluconate + NADPH
-
286278
Brevibacterium ketosoreductum ATCC21914
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
-
2-dehydro-D-gluconate + NADPH
11.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum ATCC21914
D-gluconate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
?
2-keto-L-gulonate + NADPH
10.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum
L-idonate + NADP+
-
286278
Brevibacterium ketosoreductum
?
2-keto-L-gulonate + NADPH
10.8% of activity with 2,5-diketo-D-gluconate
286278
Brevibacterium ketosoreductum ATCC21914
L-idonate + NADP+
-
286278
Brevibacterium ketosoreductum ATCC21914
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Brevibacterium ketosoreductum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
35% of activity left after incubation at pH 8.4 for 2 h at 30°C, no remaining activity below pH 5.2 or above pH 10.0
Brevibacterium ketosoreductum
Other publictions for EC 1.1.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721369
Rauch
Characterization of enzymes in ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Appl. Microbiol. Biotechnol.
88
711-718
2010
-
-
1
-
-
-
-
3
-
-
2
4
-
6
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
2
4
-
-
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-
-
-
-
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
695779
De Werra
Role of gluconic acid producti ...
Pseudomonas fluorescens, Pseudomonas fluorescens CHA0
Appl. Environ. Microbiol.
75
4162-4174
2009
-
-
1
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700265
Pajaniappan
A temperature-regulated Campyl ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Mol. Microbiol.
68
474-491
2008
1
1
1
-
1
-
-
-
1
-
2
-
-
13
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
1
1
1
1
-
1
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1
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2
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-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
684567
Toyama
Membrane-bound, 2-keto-D-gluco ...
Gluconobacter frateurii, Gluconobacter frateurii IFO 3271
Appl. Environ. Microbiol.
73
6551-6556
2007
-
-
1
-
-
-
-
-
1
1
3
2
-
3
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
3
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685666
Saichana
Preparation of enzymes require ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Biosci. Biotechnol. Biochem.
71
2478-2486
2007
-
-
1
-
-
-
-
-
1
1
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
4
-
3
-
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286279
Yum
The yiaE gene, located at 80.1 ...
Escherichia coli
J. Bacteriol.
180
5984-5988
1998
-
-
1
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5693
Pitt
Enzymes of gluconate metabolis ...
Penicillium chrysogenum, Penicillium chrysogenum Westling
Antonie van Leeuwenhoek
51
353-364
1985
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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1
2
-
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1
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-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286275
Ameyama
-
2-keto-D-Gluconate reductase f ...
Acetobacter ascendens, Acetobacter pasteurianus, Gluconacetobacter liquefaciens, Gluconobacter oxydans
Methods Enzymol.
89
203-210
1982
-
-
-
3
-
-
8
-
4
4
5
3
-
4
-
-
3
-
-
-
3
4
31
2
-
-
4
-
8
-
4
12
-
-
-
-
-
-
12
3
-
-
-
8
-
-
4
4
5
3
-
-
-
3
-
-
3
4
31
2
-
-
4
-
8
-
4
-
-
-
-
-
-
-
286274
Adachi
Crystalline 2-ketogluconate re ...
Acetobacter ascendens
Agric. Biol. Chem.
42
2057-2062
1978
-
-
-
1
-
3
1
4
1
1
2
-
-
1
-
-
1
-
-
-
1
1
7
1
1
1
3
-
2
2
-
2
-
-
-
-
-
-
2
1
-
3
-
1
-
4
1
1
2
-
-
-
-
1
-
-
1
1
7
1
1
1
3
-
2
2
-
-
-
-
-
-
-
-
286276
Shinagawa
-
Distribution of gluconate dehy ...
acetic acid bacteria, no activity in a number of oxidative or aerobic bacteria
Agric. Biol. Chem.
42
1055-1057
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
2
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2
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1
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2
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-
-
-
-
-
-
-
286273
Chiyonobu
-
Purification, crystallization ...
Acetobacter pasteurianus
Agric. Biol. Chem.
40
175-184
1976
-
-
-
1
-
2
9
12
1
1
2
1
-
1
-
-
1
-
-
-
1
2
10
1
1
1
2
-
2
2
1
2
-
-
-
-
-
-
2
1
-
2
-
9
-
12
1
1
2
1
-
-
-
1
-
-
1
2
10
1
1
1
2
-
2
2
1
-
-
-
-
-
-
-