BRENDA - Enzyme Database show
show all sequences of 1.1.1.215

2-keto-D-Gluconate reductase from acetic acid bacteria

Ameyama, M.; Adachi, O.; Methods Enzymol. 89, 203-210 (1982)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Acetobacter ascendens
-
Acetobacter pasteurianus
-
Gluconacetobacter liquefaciens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition
Acetobacter ascendens
Hg2+
complete inhibition
Acetobacter pasteurianus
Hg2+
complete inhibition
Gluconacetobacter liquefaciens
Hg2+
complete inhibition
Gluconobacter oxydans
sulfhydryl reagents
strong
Acetobacter ascendens
sulfhydryl reagents
strong
Acetobacter pasteurianus
sulfhydryl reagents
strong
Gluconacetobacter liquefaciens
sulfhydryl reagents
strong
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Acetobacter ascendens
5737
-
cytoplasm
-
Acetobacter pasteurianus
5737
-
cytoplasm
-
Gluconacetobacter liquefaciens
5737
-
cytoplasm
-
Gluconobacter oxydans
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Acetobacter ascendens
additional information
no metal requirement
Acetobacter pasteurianus
additional information
no metal requirement
Gluconacetobacter liquefaciens
additional information
no metal requirement
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 43000 + 1 * 34000, SDS-PAGE
Gluconacetobacter liquefaciens
43000
-
2 * 43000 + 1 * 34000, SDS-PAGE
Gluconacetobacter liquefaciens
120000
-
gel filtration
Acetobacter ascendens
120000
-
gel filtration
Acetobacter pasteurianus
120000
-
gel filtration
Gluconacetobacter liquefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydro-D-gluconate + NADPH
Acetobacter pasteurianus
-
D-gluconate + NADP+
-
Acetobacter pasteurianus
r
2-dehydro-D-gluconate + NADPH
Gluconobacter oxydans
reverse reaction at the same rate by Gluconobacter enzyme
D-gluconate + NADP+
-
Gluconobacter oxydans
r
2-dehydro-D-gluconate + NADPH
Gluconacetobacter liquefaciens
reverse reaction at the same rate by Gluconobacter enzyme
D-gluconate + NADP+
-
Gluconacetobacter liquefaciens
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetobacter ascendens
-
-
-
Acetobacter pasteurianus
-
-
-
Gluconacetobacter liquefaciens
-
-
-
Gluconobacter oxydans
-
-
-
Purification (Commentary)
Commentary
Organism
-
Gluconacetobacter liquefaciens
affinity chromatography on Blue-Dextran Sepharose B
Acetobacter ascendens
partial
Gluconobacter oxydans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
61.03
-
-
Gluconacetobacter liquefaciens
187
-
-
Acetobacter ascendens
336
-
-
Acetobacter pasteurianus
Storage Stability
Storage Stability
Organism
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Gluconobacter oxydans
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Acetobacter pasteurianus
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Gluconacetobacter liquefaciens
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Acetobacter ascendens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-D-gluconate + NADPH
-
286275
Acetobacter pasteurianus
D-gluconate + NADP+
-
286275
Acetobacter pasteurianus
r
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286275
Gluconobacter oxydans
D-gluconate + NADP+
-
286275
Gluconobacter oxydans
r
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286275
Gluconacetobacter liquefaciens
D-gluconate + NADP+
-
286275
Gluconacetobacter liquefaciens
r
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286275
Acetobacter ascendens
D-gluconate + NADP+
-
286275
Acetobacter ascendens
r
2-dehydro-D-gluconate + NADPH
strongly preferred reaction of Acetobacter enzyme
286275
Acetobacter pasteurianus
D-gluconate + NADP+
-
286275
Acetobacter pasteurianus
r
2-dehydro-D-gluconate + NADPH
strongly preferred reaction of Acetobacter enzyme
286275
Acetobacter ascendens
D-gluconate + NADP+
-
286275
Acetobacter ascendens
r
2-dehydro-D-gluconate + NADPH
reverse reaction at the same rate by Gluconobacter enzyme
286275
Gluconobacter oxydans
D-gluconate + NADP+
-
286275
Gluconobacter oxydans
r
2-dehydro-D-gluconate + NADPH
reverse reaction at the same rate by Gluconobacter enzyme
286275
Gluconacetobacter liquefaciens
D-gluconate + NADP+
-
286275
Gluconacetobacter liquefaciens
r
2-keto-L-gulonate + NADPH
third best substrate
286275
Gluconobacter oxydans
L-idonate + NADP+
-
-
-
r
2-keto-L-gulonate + NADPH
third best substrate
286275
Gluconacetobacter liquefaciens
L-idonate + NADP+
-
-
-
r
2-keto-L-gulonate + NADPH
third best substrate
286275
Acetobacter ascendens
L-idonate + NADP+
-
-
-
r
2-keto-L-gulonate + NADPH
betst substrate
286275
Acetobacter pasteurianus
L-idonate + NADP+
-
-
-
r
5-keto-D-gluconate + NADPH
-
286275
Gluconobacter oxydans
D-gluconate + NADP+
-
286275
Gluconobacter oxydans
?
5-keto-D-gluconate + NADPH
-
286275
Gluconacetobacter liquefaciens
D-gluconate + NADP+
-
286275
Gluconacetobacter liquefaciens
?
5-keto-D-gluconate + NADPH
poor substrate
286275
Acetobacter pasteurianus
D-gluconate + NADP+
-
286275
Acetobacter pasteurianus
?
5-keto-D-gluconate + NADPH
poor substrate
286275
Acetobacter ascendens
D-gluconate + NADP+
-
286275
Acetobacter ascendens
?
D-galactonate + NADP+
-
286275
Acetobacter pasteurianus
2-dehydro-D-galactonate + NADPH
-
286275
Acetobacter pasteurianus
r
D-galactonate + NADP+
-
286275
Acetobacter ascendens
2-dehydro-D-galactonate + NADPH
-
286275
Acetobacter ascendens
r
D-galactonate + NADP+
third best substrate
286275
Gluconobacter oxydans
2-dehydro-D-galactonate + NADPH
-
286275
Gluconobacter oxydans
r
D-galactonate + NADP+
third best substrate
286275
Gluconacetobacter liquefaciens
2-dehydro-D-galactonate + NADPH
-
286275
Gluconacetobacter liquefaciens
r
D-xylonate + NADPH
-
286275
Gluconobacter oxydans
2-keto-D-xylonate + NADP+
-
286275
Gluconobacter oxydans
?
D-xylonate + NADPH
-
286275
Acetobacter pasteurianus
2-keto-D-xylonate + NADP+
-
286275
Acetobacter pasteurianus
?
D-xylonate + NADPH
-
286275
Gluconacetobacter liquefaciens
2-keto-D-xylonate + NADP+
-
286275
Gluconacetobacter liquefaciens
?
L-idonate + NADP+
poor substrate
286275
Acetobacter pasteurianus
2-keto-L-idonate + NADPH
-
286275
Acetobacter pasteurianus
-
L-idonate + NADP+
poor substrate
286275
Acetobacter ascendens
2-keto-L-idonate + NADPH
-
286275
Acetobacter ascendens
-
L-idonate + NADP+
best substrate
286275
Gluconobacter oxydans
2-keto-L-idonate + NADPH
-
286275
Gluconobacter oxydans
-
L-idonate + NADP+
best substrate
286275
Gluconacetobacter liquefaciens
2-keto-L-idonate + NADPH
-
286275
Gluconacetobacter liquefaciens
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Gluconobacter oxydans
?
-
-
-
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Acetobacter pasteurianus
?
-
-
-
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Gluconacetobacter liquefaciens
?
-
-
-
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Acetobacter ascendens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
trimer
-
Acetobacter ascendens
trimer
2 * 43000 + 1 * 34000, SDS-PAGE
Gluconacetobacter liquefaciens
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
stable for 5 min, enhanced by the addition of substrate
Acetobacter ascendens
55
-
stable for 5 min, enhanced by the addition of substrate
Acetobacter pasteurianus
55
-
stable for 5 min, enhanced by the addition of substrate
Gluconacetobacter liquefaciens
55
-
stable for 5 min, enhanced by the addition of substrate
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of 2-ketogluconate
Acetobacter ascendens
6
-
reduction of 2-ketogluconate
Acetobacter pasteurianus
6
-
reduction of 2-ketogluconate
Gluconacetobacter liquefaciens
6
-
reduction of 2-ketogluconate
Gluconobacter oxydans
10.5
-
-
Acetobacter ascendens
10.5
-
oxidation of gluconate
Gluconacetobacter liquefaciens
11
-
oxidation of gluconate
Acetobacter ascendens
12
-
oxidation of gluconate
Acetobacter pasteurianus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Acetobacter ascendens
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Acetobacter pasteurianus
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Gluconacetobacter liquefaciens
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Acetobacter ascendens
additional information
no activity with NADH
Acetobacter pasteurianus
additional information
no activity with NADH
Gluconacetobacter liquefaciens
additional information
no activity with NADH
Gluconobacter oxydans
NADP+
-
Acetobacter ascendens
NADP+
-
Acetobacter pasteurianus
NADP+
-
Gluconacetobacter liquefaciens
NADP+
-
Gluconobacter oxydans
NADPH
-
Acetobacter ascendens
NADPH
-
Acetobacter pasteurianus
NADPH
-
Gluconacetobacter liquefaciens
NADPH
-
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Acetobacter ascendens
additional information
no activity with NADH
Acetobacter pasteurianus
additional information
no activity with NADH
Gluconacetobacter liquefaciens
additional information
no activity with NADH
Gluconobacter oxydans
NADP+
-
Acetobacter ascendens
NADP+
-
Acetobacter pasteurianus
NADP+
-
Gluconacetobacter liquefaciens
NADP+
-
Gluconobacter oxydans
NADPH
-
Acetobacter ascendens
NADPH
-
Acetobacter pasteurianus
NADPH
-
Gluconacetobacter liquefaciens
NADPH
-
Gluconobacter oxydans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Acetobacter ascendens
-
Acetobacter pasteurianus
-
Gluconacetobacter liquefaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition
Acetobacter ascendens
Hg2+
complete inhibition
Acetobacter pasteurianus
Hg2+
complete inhibition
Gluconacetobacter liquefaciens
Hg2+
complete inhibition
Gluconobacter oxydans
sulfhydryl reagents
strong
Acetobacter ascendens
sulfhydryl reagents
strong
Acetobacter pasteurianus
sulfhydryl reagents
strong
Gluconacetobacter liquefaciens
sulfhydryl reagents
strong
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Acetobacter ascendens
5737
-
cytoplasm
-
Acetobacter pasteurianus
5737
-
cytoplasm
-
Gluconacetobacter liquefaciens
5737
-
cytoplasm
-
Gluconobacter oxydans
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Acetobacter ascendens
additional information
no metal requirement
Acetobacter pasteurianus
additional information
no metal requirement
Gluconacetobacter liquefaciens
additional information
no metal requirement
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 43000 + 1 * 34000, SDS-PAGE
Gluconacetobacter liquefaciens
43000
-
2 * 43000 + 1 * 34000, SDS-PAGE
Gluconacetobacter liquefaciens
120000
-
gel filtration
Acetobacter ascendens
120000
-
gel filtration
Acetobacter pasteurianus
120000
-
gel filtration
Gluconacetobacter liquefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydro-D-gluconate + NADPH
Acetobacter pasteurianus
-
D-gluconate + NADP+
-
Acetobacter pasteurianus
r
2-dehydro-D-gluconate + NADPH
Gluconobacter oxydans
reverse reaction at the same rate by Gluconobacter enzyme
D-gluconate + NADP+
-
Gluconobacter oxydans
r
2-dehydro-D-gluconate + NADPH
Gluconacetobacter liquefaciens
reverse reaction at the same rate by Gluconobacter enzyme
D-gluconate + NADP+
-
Gluconacetobacter liquefaciens
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gluconacetobacter liquefaciens
affinity chromatography on Blue-Dextran Sepharose B
Acetobacter ascendens
partial
Gluconobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
61.03
-
-
Gluconacetobacter liquefaciens
187
-
-
Acetobacter ascendens
336
-
-
Acetobacter pasteurianus
Storage Stability (protein specific)
Storage Stability
Organism
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Gluconobacter oxydans
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Acetobacter pasteurianus
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Gluconacetobacter liquefaciens
5C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol
Acetobacter ascendens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-D-gluconate + NADPH
-
286275
Acetobacter pasteurianus
D-gluconate + NADP+
-
286275
Acetobacter pasteurianus
r
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286275
Gluconobacter oxydans
D-gluconate + NADP+
-
286275
Gluconobacter oxydans
r
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286275
Gluconacetobacter liquefaciens
D-gluconate + NADP+
-
286275
Gluconacetobacter liquefaciens
r
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286275
Acetobacter ascendens
D-gluconate + NADP+
-
286275
Acetobacter ascendens
r
2-dehydro-D-gluconate + NADPH
strongly preferred reaction of Acetobacter enzyme
286275
Acetobacter pasteurianus
D-gluconate + NADP+
-
286275
Acetobacter pasteurianus
r
2-dehydro-D-gluconate + NADPH
strongly preferred reaction of Acetobacter enzyme
286275
Acetobacter ascendens
D-gluconate + NADP+
-
286275
Acetobacter ascendens
r
2-dehydro-D-gluconate + NADPH
reverse reaction at the same rate by Gluconobacter enzyme
286275
Gluconobacter oxydans
D-gluconate + NADP+
-
286275
Gluconobacter oxydans
r
2-dehydro-D-gluconate + NADPH
reverse reaction at the same rate by Gluconobacter enzyme
286275
Gluconacetobacter liquefaciens
D-gluconate + NADP+
-
286275
Gluconacetobacter liquefaciens
r
2-keto-L-gulonate + NADPH
third best substrate
286275
Gluconobacter oxydans
L-idonate + NADP+
-
-
-
r
2-keto-L-gulonate + NADPH
third best substrate
286275
Gluconacetobacter liquefaciens
L-idonate + NADP+
-
-
-
r
2-keto-L-gulonate + NADPH
third best substrate
286275
Acetobacter ascendens
L-idonate + NADP+
-
-
-
r
2-keto-L-gulonate + NADPH
betst substrate
286275
Acetobacter pasteurianus
L-idonate + NADP+
-
-
-
r
5-keto-D-gluconate + NADPH
-
286275
Gluconobacter oxydans
D-gluconate + NADP+
-
286275
Gluconobacter oxydans
?
5-keto-D-gluconate + NADPH
-
286275
Gluconacetobacter liquefaciens
D-gluconate + NADP+
-
286275
Gluconacetobacter liquefaciens
?
5-keto-D-gluconate + NADPH
poor substrate
286275
Acetobacter pasteurianus
D-gluconate + NADP+
-
286275
Acetobacter pasteurianus
?
5-keto-D-gluconate + NADPH
poor substrate
286275
Acetobacter ascendens
D-gluconate + NADP+
-
286275
Acetobacter ascendens
?
D-galactonate + NADP+
-
286275
Acetobacter pasteurianus
2-dehydro-D-galactonate + NADPH
-
286275
Acetobacter pasteurianus
r
D-galactonate + NADP+
-
286275
Acetobacter ascendens
2-dehydro-D-galactonate + NADPH
-
286275
Acetobacter ascendens
r
D-galactonate + NADP+
third best substrate
286275
Gluconobacter oxydans
2-dehydro-D-galactonate + NADPH
-
286275
Gluconobacter oxydans
r
D-galactonate + NADP+
third best substrate
286275
Gluconacetobacter liquefaciens
2-dehydro-D-galactonate + NADPH
-
286275
Gluconacetobacter liquefaciens
r
D-xylonate + NADPH
-
286275
Gluconobacter oxydans
2-keto-D-xylonate + NADP+
-
286275
Gluconobacter oxydans
?
D-xylonate + NADPH
-
286275
Acetobacter pasteurianus
2-keto-D-xylonate + NADP+
-
286275
Acetobacter pasteurianus
?
D-xylonate + NADPH
-
286275
Gluconacetobacter liquefaciens
2-keto-D-xylonate + NADP+
-
286275
Gluconacetobacter liquefaciens
?
L-idonate + NADP+
poor substrate
286275
Acetobacter pasteurianus
2-keto-L-idonate + NADPH
-
286275
Acetobacter pasteurianus
-
L-idonate + NADP+
poor substrate
286275
Acetobacter ascendens
2-keto-L-idonate + NADPH
-
286275
Acetobacter ascendens
-
L-idonate + NADP+
best substrate
286275
Gluconobacter oxydans
2-keto-L-idonate + NADPH
-
286275
Gluconobacter oxydans
-
L-idonate + NADP+
best substrate
286275
Gluconacetobacter liquefaciens
2-keto-L-idonate + NADPH
-
286275
Gluconacetobacter liquefaciens
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Gluconobacter oxydans
?
-
-
-
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Acetobacter pasteurianus
?
-
-
-
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Gluconacetobacter liquefaciens
?
-
-
-
-
additional information
no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate
286275
Acetobacter ascendens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
-
Acetobacter ascendens
trimer
2 * 43000 + 1 * 34000, SDS-PAGE
Gluconacetobacter liquefaciens
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
stable for 5 min, enhanced by the addition of substrate
Acetobacter ascendens
55
-
stable for 5 min, enhanced by the addition of substrate
Acetobacter pasteurianus
55
-
stable for 5 min, enhanced by the addition of substrate
Gluconacetobacter liquefaciens
55
-
stable for 5 min, enhanced by the addition of substrate
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of 2-ketogluconate
Acetobacter ascendens
6
-
reduction of 2-ketogluconate
Acetobacter pasteurianus
6
-
reduction of 2-ketogluconate
Gluconacetobacter liquefaciens
6
-
reduction of 2-ketogluconate
Gluconobacter oxydans
10.5
-
-
Acetobacter ascendens
10.5
-
oxidation of gluconate
Gluconacetobacter liquefaciens
11
-
oxidation of gluconate
Acetobacter ascendens
12
-
oxidation of gluconate
Acetobacter pasteurianus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Acetobacter ascendens
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Acetobacter pasteurianus
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Gluconacetobacter liquefaciens
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5C
Gluconobacter oxydans
Other publictions for EC 1.1.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721369
Rauch
Characterization of enzymes in ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Appl. Microbiol. Biotechnol.
88
711-718
2010
-
-
1
-
-
-
-
3
-
-
2
4
-
6
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
2
4
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
695779
De Werra
Role of gluconic acid producti ...
Pseudomonas fluorescens, Pseudomonas fluorescens CHA0
Appl. Environ. Microbiol.
75
4162-4174
2009
-
-
1
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700265
Pajaniappan
A temperature-regulated Campyl ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Mol. Microbiol.
68
474-491
2008
1
1
1
-
1
-
-
-
1
-
2
-
-
13
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
1
1
1
1
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
684567
Toyama
Membrane-bound, 2-keto-D-gluco ...
Gluconobacter frateurii, Gluconobacter frateurii IFO 3271
Appl. Environ. Microbiol.
73
6551-6556
2007
-
-
1
-
-
-
-
-
1
1
3
2
-
3
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
3
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685666
Saichana
Preparation of enzymes require ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Biosci. Biotechnol. Biochem.
71
2478-2486
2007
-
-
1
-
-
-
-
-
1
1
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
4
-
3
-
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286279
Yum
The yiaE gene, located at 80.1 ...
Escherichia coli
J. Bacteriol.
180
5984-5988
1998
-
-
1
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5693
Pitt
Enzymes of gluconate metabolis ...
Penicillium chrysogenum, Penicillium chrysogenum Westling
Antonie van Leeuwenhoek
51
353-364
1985
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286275
Ameyama
-
2-keto-D-Gluconate reductase f ...
Acetobacter ascendens, Acetobacter pasteurianus, Gluconacetobacter liquefaciens, Gluconobacter oxydans
Methods Enzymol.
89
203-210
1982
-
-
-
3
-
-
8
-
4
4
5
3
-
4
-
-
3
-
-
-
3
4
31
2
-
-
4
-
8
-
4
12
-
-
-
-
-
-
12
3
-
-
-
8
-
-
4
4
5
3
-
-
-
3
-
-
3
4
31
2
-
-
4
-
8
-
4
-
-
-
-
-
-
-
286274
Adachi
Crystalline 2-ketogluconate re ...
Acetobacter ascendens
Agric. Biol. Chem.
42
2057-2062
1978
-
-
-
1
-
3
1
4
1
1
2
-
-
1
-
-
1
-
-
-
1
1
7
1
1
1
3
-
2
2
-
2
-
-
-
-
-
-
2
1
-
3
-
1
-
4
1
1
2
-
-
-
-
1
-
-
1
1
7
1
1
1
3
-
2
2
-
-
-
-
-
-
-
-
286276
Shinagawa
-
Distribution of gluconate dehy ...
acetic acid bacteria, no activity in a number of oxidative or aerobic bacteria
Agric. Biol. Chem.
42
1055-1057
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
2
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-
-
-
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-
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-
2
-
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-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
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-
-
-
1
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-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286273
Chiyonobu
-
Purification, crystallization ...
Acetobacter pasteurianus
Agric. Biol. Chem.
40
175-184
1976
-
-
-
1
-
2
9
12
1
1
2
1
-
1
-
-
1
-
-
-
1
2
10
1
1
1
2
-
2
2
1
2
-
-
-
-
-
-
2
1
-
2
-
9
-
12
1
1
2
1
-
-
-
1
-
-
1
2
10
1
1
1
2
-
2
2
1
-
-
-
-
-
-
-