BRENDA - Enzyme Database show
show all sequences of 1.1.1.215

Crystalline 2-ketogluconate reductase from Acetobacter ascendens, the second instance of crystalline enzyme in genus Acetobacter

Adachi, O.; Chiyonobu, T.; Shinagawa, E.; Matsushita, K.; Ameyama, M.; Agric. Biol. Chem. 42, 2057-2062 (1978)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Acetobacter ascendens
General Stability
General Stability
Organism
2-ketogluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l
Acetobacter ascendens
5-ketogluconate does not stabilize
Acetobacter ascendens
gluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l
Acetobacter ascendens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition
Acetobacter ascendens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0074
-
NADPH
-
Acetobacter ascendens
1.2
-
NADP+
-
Acetobacter ascendens
4.4
-
gluconate
-
Acetobacter ascendens
5.3
-
2-keto-D-gluconate
-
Acetobacter ascendens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Acetobacter ascendens
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Acetobacter ascendens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
3 * 40000, SDS-PAGE
Acetobacter ascendens
120000
-
gel filtration
Acetobacter ascendens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetobacter ascendens
-
-
-
Purification (Commentary)
Commentary
Organism
affinity chromatography on Blue-Dextran Sepharose B
Acetobacter ascendens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
187
-
-
Acetobacter ascendens
Storage Stability
Storage Stability
Organism
ammonium sulfate solution of crystalline enzyme stable for over 2 years
Acetobacter ascendens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286274
Acetobacter ascendens
D-gluconate + NADP+
-
286274
Acetobacter ascendens
r
2-dehydro-D-gluconate + NADPH
equal reaction rate of reduction of 2-ketogluconate and oxidation of gluconate with Gluconobacter enzyme
286274
Acetobacter ascendens
D-gluconate + NADP+
-
286274
Acetobacter ascendens
r
2-keto-L-gulonate + NADPH
-
286274
Acetobacter ascendens
L-idonate + NADP+
-
-
-
r
5-keto-D-gluconate + NADPH
poor substrate
286274
Acetobacter ascendens
D-gluconate + NADP+
-
286274
Acetobacter ascendens
?
D-galactonate + NADP+
-
286274
Acetobacter ascendens
2-dehydro-D-galactonate + NADPH
-
286274
Acetobacter ascendens
r
L-idonate + NADP+
-
286274
Acetobacter ascendens
2-keto-L-idonate + NADPH
-
286274
Acetobacter ascendens
-
additional information
D-xylonate, D-glucose, D-fructose, L-sorbose, 5-keto-D-fructose, D-sorbitol, glycerol
286274
Acetobacter ascendens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
trimer
3 * 40000, SDS-PAGE
Acetobacter ascendens
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Acetobacter ascendens
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
65
about half-maximal activity at 35C and 65C
Acetobacter ascendens
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
42
-
stable for 10 min, oxidation activity
Acetobacter ascendens
53
-
stable for 10 min, reduction activity
Acetobacter ascendens
70
-
stable for 10 min in the presence of either 2-ketogluconate or gluconate
Acetobacter ascendens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of 2-ketogluconate
Acetobacter ascendens
10.5
-
-
Acetobacter ascendens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
8.5
about half-maximal activity at pH 4.5 and 8.5, reduction of ketogluconate
Acetobacter ascendens
10
12
about half-maximal activity at pH 10 and 12, oxidation of gluconate
Acetobacter ascendens
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Acetobacter ascendens
NADPH
-
Acetobacter ascendens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Acetobacter ascendens
NADPH
-
Acetobacter ascendens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Acetobacter ascendens
General Stability (protein specific)
General Stability
Organism
2-ketogluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l
Acetobacter ascendens
5-ketogluconate does not stabilize
Acetobacter ascendens
gluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l
Acetobacter ascendens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition
Acetobacter ascendens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0074
-
NADPH
-
Acetobacter ascendens
1.2
-
NADP+
-
Acetobacter ascendens
4.4
-
gluconate
-
Acetobacter ascendens
5.3
-
2-keto-D-gluconate
-
Acetobacter ascendens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Acetobacter ascendens
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Acetobacter ascendens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
3 * 40000, SDS-PAGE
Acetobacter ascendens
120000
-
gel filtration
Acetobacter ascendens
Purification (Commentary) (protein specific)
Commentary
Organism
affinity chromatography on Blue-Dextran Sepharose B
Acetobacter ascendens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
187
-
-
Acetobacter ascendens
Storage Stability (protein specific)
Storage Stability
Organism
ammonium sulfate solution of crystalline enzyme stable for over 2 years
Acetobacter ascendens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-D-gluconate + NADPH
i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter
286274
Acetobacter ascendens
D-gluconate + NADP+
-
286274
Acetobacter ascendens
r
2-dehydro-D-gluconate + NADPH
equal reaction rate of reduction of 2-ketogluconate and oxidation of gluconate with Gluconobacter enzyme
286274
Acetobacter ascendens
D-gluconate + NADP+
-
286274
Acetobacter ascendens
r
2-keto-L-gulonate + NADPH
-
286274
Acetobacter ascendens
L-idonate + NADP+
-
-
-
r
5-keto-D-gluconate + NADPH
poor substrate
286274
Acetobacter ascendens
D-gluconate + NADP+
-
286274
Acetobacter ascendens
?
D-galactonate + NADP+
-
286274
Acetobacter ascendens
2-dehydro-D-galactonate + NADPH
-
286274
Acetobacter ascendens
r
L-idonate + NADP+
-
286274
Acetobacter ascendens
2-keto-L-idonate + NADPH
-
286274
Acetobacter ascendens
-
additional information
D-xylonate, D-glucose, D-fructose, L-sorbose, 5-keto-D-fructose, D-sorbitol, glycerol
286274
Acetobacter ascendens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 40000, SDS-PAGE
Acetobacter ascendens
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Acetobacter ascendens
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
65
about half-maximal activity at 35C and 65C
Acetobacter ascendens
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
42
-
stable for 10 min, oxidation activity
Acetobacter ascendens
53
-
stable for 10 min, reduction activity
Acetobacter ascendens
70
-
stable for 10 min in the presence of either 2-ketogluconate or gluconate
Acetobacter ascendens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of 2-ketogluconate
Acetobacter ascendens
10.5
-
-
Acetobacter ascendens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
8.5
about half-maximal activity at pH 4.5 and 8.5, reduction of ketogluconate
Acetobacter ascendens
10
12
about half-maximal activity at pH 10 and 12, oxidation of gluconate
Acetobacter ascendens
Other publictions for EC 1.1.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721369
Rauch
Characterization of enzymes in ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Appl. Microbiol. Biotechnol.
88
711-718
2010
-
-
1
-
-
-
-
3
-
-
2
4
-
6
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
2
4
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
695779
De Werra
Role of gluconic acid producti ...
Pseudomonas fluorescens, Pseudomonas fluorescens CHA0
Appl. Environ. Microbiol.
75
4162-4174
2009
-
-
1
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700265
Pajaniappan
A temperature-regulated Campyl ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Mol. Microbiol.
68
474-491
2008
1
1
1
-
1
-
-
-
1
-
2
-
-
13
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
1
1
1
1
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
684567
Toyama
Membrane-bound, 2-keto-D-gluco ...
Gluconobacter frateurii, Gluconobacter frateurii IFO 3271
Appl. Environ. Microbiol.
73
6551-6556
2007
-
-
1
-
-
-
-
-
1
1
3
2
-
3
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
3
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685666
Saichana
Preparation of enzymes require ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Biosci. Biotechnol. Biochem.
71
2478-2486
2007
-
-
1
-
-
-
-
-
1
1
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
4
-
3
-
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286279
Yum
The yiaE gene, located at 80.1 ...
Escherichia coli
J. Bacteriol.
180
5984-5988
1998
-
-
1
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5693
Pitt
Enzymes of gluconate metabolis ...
Penicillium chrysogenum, Penicillium chrysogenum Westling
Antonie van Leeuwenhoek
51
353-364
1985
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286275
Ameyama
-
2-keto-D-Gluconate reductase f ...
Acetobacter ascendens, Acetobacter pasteurianus, Gluconacetobacter liquefaciens, Gluconobacter oxydans
Methods Enzymol.
89
203-210
1982
-
-
-
3
-
-
8
-
4
4
5
3
-
4
-
-
3
-
-
-
3
4
31
2
-
-
4
-
8
-
4
12
-
-
-
-
-
-
12
3
-
-
-
8
-
-
4
4
5
3
-
-
-
3
-
-
3
4
31
2
-
-
4
-
8
-
4
-
-
-
-
-
-
-
286274
Adachi
Crystalline 2-ketogluconate re ...
Acetobacter ascendens
Agric. Biol. Chem.
42
2057-2062
1978
-
-
-
1
-
3
1
4
1
1
2
-
-
1
-
-
1
-
-
-
1
1
7
1
1
1
3
-
2
2
-
2
-
-
-
-
-
-
2
1
-
3
-
1
-
4
1
1
2
-
-
-
-
1
-
-
1
1
7
1
1
1
3
-
2
2
-
-
-
-
-
-
-
-
286276
Shinagawa
-
Distribution of gluconate dehy ...
acetic acid bacteria, no activity in a number of oxidative or aerobic bacteria
Agric. Biol. Chem.
42
1055-1057
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286273
Chiyonobu
-
Purification, crystallization ...
Acetobacter pasteurianus
Agric. Biol. Chem.
40
175-184
1976
-
-
-
1
-
2
9
12
1
1
2
1
-
1
-
-
1
-
-
-
1
2
10
1
1
1
2
-
2
2
1
2
-
-
-
-
-
-
2
1
-
2
-
9
-
12
1
1
2
1
-
-
-
1
-
-
1
2
10
1
1
1
2
-
2
2
1
-
-
-
-
-
-
-