BRENDA - Enzyme Database show
show all sequences of 1.1.1.215

Purification, crystallization and properties of 2-ketogluconate reductase from Acetobacter rancens

Chiyonobu, T.; Shinagawa, E.; Adachi, O.; Ameyama, M.; Agric. Biol. Chem. 40, 175-184 (1976)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Acetobacter pasteurianus
General Stability
General Stability
Organism
2-mercaptoethanol stabilizes
Acetobacter pasteurianus
dialysis, stable to
Acetobacter pasteurianus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Acetobacter pasteurianus
Co2+
-
Acetobacter pasteurianus
Hg2+
complete inhibition
Acetobacter pasteurianus
additional information
not inhibited by monoiodoacetate
Acetobacter pasteurianus
oxalate
-
Acetobacter pasteurianus
oxamate
slight inhibition of reverse reaction
Acetobacter pasteurianus
pyruvate
weak
Acetobacter pasteurianus
Tartronate
slight inhibition of reverse reaction
Acetobacter pasteurianus
Zn2+
-
Acetobacter pasteurianus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
+ 5-keto-D-gluconate
Acetobacter pasteurianus
0.062
-
NADPH
+ 2-keto-D-galactonate
Acetobacter pasteurianus
0.065
-
Hydroxypyruvate
-
Acetobacter pasteurianus
0.083
-
NADP+
+ D-gluconate
Acetobacter pasteurianus
0.11
-
NADPH
+ 2-keto-L-gulonate
Acetobacter pasteurianus
0.14
-
NADPH
+ hydroxypyruvate
Acetobacter pasteurianus
0.38
-
glyoxylate
-
Acetobacter pasteurianus
0.71
-
NADPH
cosubstrate glyoxylate
Acetobacter pasteurianus
0.86
-
5-Keto-D-gluconate
-
Acetobacter pasteurianus
2.4
-
D-gluconate
-
Acetobacter pasteurianus
16
-
2-keto-D-galactonate
-
Acetobacter pasteurianus
91
-
2-keto-L-gulonate
-
Acetobacter pasteurianus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Acetobacter pasteurianus
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Acetobacter pasteurianus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15000
-
8 * 15000, SDS-PAGE
Acetobacter pasteurianus
120000
-
gel filtration
Acetobacter pasteurianus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydro-D-gluconate + NADPH
Acetobacter pasteurianus
-
D-gluconate + NADP+
-
Acetobacter pasteurianus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetobacter pasteurianus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Acetobacter pasteurianus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
336
-
-
Acetobacter pasteurianus
Storage Stability
Storage Stability
Organism
-20°C, stable in the presence of sulfhydryl agents and ammonium sulfate
Acetobacter pasteurianus
0°C, stable for at least a month in phosphate buffer
Acetobacter pasteurianus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-D-gluconate + NADPH
-
286273
Acetobacter pasteurianus
D-gluconate + NADP+
-
286273
Acetobacter pasteurianus
r
2-keto-L-gulonate + NADPH
-
286273
Acetobacter pasteurianus
L-idonate + NADP+
-
-
-
r
5-keto-D-gluconate + NADPH
poor substrate
286273
Acetobacter pasteurianus
D-gluconate + NADP+
-
286273
Acetobacter pasteurianus
?
acetaldehyde + NADPH + H+
poor substrate
286273
Acetobacter pasteurianus
ethanol + NADP+
-
-
-
?
D-galactonate + NADP+
-
286273
Acetobacter pasteurianus
2-dehydro-D-galactonate + NADPH
-
286273
Acetobacter pasteurianus
r
glyoxal + NADPH
reduction at 33% the rate of 2-ketogluconate reduction
286273
Acetobacter pasteurianus
?
-
-
-
?
glyoxylate + NADPH
reduction at 350% the rate of 2-ketogluconate reduction
286273
Acetobacter pasteurianus
glycolate + NADP+
-
286273
Acetobacter pasteurianus
?
hydroxypyruvate + NADPH
reduction at 733% the rate of 2-keto-gluconate reduction
286273
Acetobacter pasteurianus
2,3-dihydroxypropanoate + NADP+
-
286273
Acetobacter pasteurianus
?
L-idonate + NADP+
-
286273
Acetobacter pasteurianus
2-keto-L-idonate + NADPH
-
286273
Acetobacter pasteurianus
-
pyruvate + NADPH + H+
reduction at 7% the rate of 2-ketogluconate reduction
286273
Acetobacter pasteurianus
lactate + NADP+
-
286273
Acetobacter pasteurianus
?
Subunits
Subunits
Commentary
Organism
octamer
8 * 15000, SDS-PAGE
Acetobacter pasteurianus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Acetobacter pasteurianus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
55
about half-maximal activity at 40°C and 55°C
Acetobacter pasteurianus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
stable for 10 min
Acetobacter pasteurianus
60
-
rapid inactivation
Acetobacter pasteurianus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
reduction of 2-ketogluconate
Acetobacter pasteurianus
12
-
-
Acetobacter pasteurianus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9.2
about half-maximal activity at pH 5.0 and 9.2, reduction of ketogluconate
Acetobacter pasteurianus
10.8
12.5
about half-maximal activity at pH 10.8 and 12.5, oxidation of gluconate
Acetobacter pasteurianus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
stable for a week in the cold, increasing inactivation below and above, stable for a month or more with additional 2-mercaptoethanol
Acetobacter pasteurianus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Acetobacter pasteurianus
NADPH
-
Acetobacter pasteurianus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Acetobacter pasteurianus
NADPH
-
Acetobacter pasteurianus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Acetobacter pasteurianus
General Stability (protein specific)
General Stability
Organism
2-mercaptoethanol stabilizes
Acetobacter pasteurianus
dialysis, stable to
Acetobacter pasteurianus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Acetobacter pasteurianus
Co2+
-
Acetobacter pasteurianus
Hg2+
complete inhibition
Acetobacter pasteurianus
additional information
not inhibited by monoiodoacetate
Acetobacter pasteurianus
oxalate
-
Acetobacter pasteurianus
oxamate
slight inhibition of reverse reaction
Acetobacter pasteurianus
pyruvate
weak
Acetobacter pasteurianus
Tartronate
slight inhibition of reverse reaction
Acetobacter pasteurianus
Zn2+
-
Acetobacter pasteurianus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
+ 5-keto-D-gluconate
Acetobacter pasteurianus
0.062
-
NADPH
+ 2-keto-D-galactonate
Acetobacter pasteurianus
0.065
-
Hydroxypyruvate
-
Acetobacter pasteurianus
0.083
-
NADP+
+ D-gluconate
Acetobacter pasteurianus
0.11
-
NADPH
+ 2-keto-L-gulonate
Acetobacter pasteurianus
0.14
-
NADPH
+ hydroxypyruvate
Acetobacter pasteurianus
0.38
-
glyoxylate
-
Acetobacter pasteurianus
0.71
-
NADPH
cosubstrate glyoxylate
Acetobacter pasteurianus
0.86
-
5-Keto-D-gluconate
-
Acetobacter pasteurianus
2.4
-
D-gluconate
-
Acetobacter pasteurianus
16
-
2-keto-D-galactonate
-
Acetobacter pasteurianus
91
-
2-keto-L-gulonate
-
Acetobacter pasteurianus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Acetobacter pasteurianus
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Acetobacter pasteurianus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15000
-
8 * 15000, SDS-PAGE
Acetobacter pasteurianus
120000
-
gel filtration
Acetobacter pasteurianus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydro-D-gluconate + NADPH
Acetobacter pasteurianus
-
D-gluconate + NADP+
-
Acetobacter pasteurianus
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acetobacter pasteurianus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
336
-
-
Acetobacter pasteurianus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable in the presence of sulfhydryl agents and ammonium sulfate
Acetobacter pasteurianus
0°C, stable for at least a month in phosphate buffer
Acetobacter pasteurianus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-D-gluconate + NADPH
-
286273
Acetobacter pasteurianus
D-gluconate + NADP+
-
286273
Acetobacter pasteurianus
r
2-keto-L-gulonate + NADPH
-
286273
Acetobacter pasteurianus
L-idonate + NADP+
-
-
-
r
5-keto-D-gluconate + NADPH
poor substrate
286273
Acetobacter pasteurianus
D-gluconate + NADP+
-
286273
Acetobacter pasteurianus
?
acetaldehyde + NADPH + H+
poor substrate
286273
Acetobacter pasteurianus
ethanol + NADP+
-
-
-
?
D-galactonate + NADP+
-
286273
Acetobacter pasteurianus
2-dehydro-D-galactonate + NADPH
-
286273
Acetobacter pasteurianus
r
glyoxal + NADPH
reduction at 33% the rate of 2-ketogluconate reduction
286273
Acetobacter pasteurianus
?
-
-
-
?
glyoxylate + NADPH
reduction at 350% the rate of 2-ketogluconate reduction
286273
Acetobacter pasteurianus
glycolate + NADP+
-
286273
Acetobacter pasteurianus
?
hydroxypyruvate + NADPH
reduction at 733% the rate of 2-keto-gluconate reduction
286273
Acetobacter pasteurianus
2,3-dihydroxypropanoate + NADP+
-
286273
Acetobacter pasteurianus
?
L-idonate + NADP+
-
286273
Acetobacter pasteurianus
2-keto-L-idonate + NADPH
-
286273
Acetobacter pasteurianus
-
pyruvate + NADPH + H+
reduction at 7% the rate of 2-ketogluconate reduction
286273
Acetobacter pasteurianus
lactate + NADP+
-
286273
Acetobacter pasteurianus
?
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
8 * 15000, SDS-PAGE
Acetobacter pasteurianus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Acetobacter pasteurianus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
55
about half-maximal activity at 40°C and 55°C
Acetobacter pasteurianus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
stable for 10 min
Acetobacter pasteurianus
60
-
rapid inactivation
Acetobacter pasteurianus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
reduction of 2-ketogluconate
Acetobacter pasteurianus
12
-
-
Acetobacter pasteurianus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9.2
about half-maximal activity at pH 5.0 and 9.2, reduction of ketogluconate
Acetobacter pasteurianus
10.8
12.5
about half-maximal activity at pH 10.8 and 12.5, oxidation of gluconate
Acetobacter pasteurianus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
stable for a week in the cold, increasing inactivation below and above, stable for a month or more with additional 2-mercaptoethanol
Acetobacter pasteurianus
Other publictions for EC 1.1.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721369
Rauch
Characterization of enzymes in ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Appl. Microbiol. Biotechnol.
88
711-718
2010
-
-
1
-
-
-
-
3
-
-
2
4
-
6
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
2
4
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
695779
De Werra
Role of gluconic acid producti ...
Pseudomonas fluorescens, Pseudomonas fluorescens CHA0
Appl. Environ. Microbiol.
75
4162-4174
2009
-
-
1
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700265
Pajaniappan
A temperature-regulated Campyl ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Mol. Microbiol.
68
474-491
2008
1
1
1
-
1
-
-
-
1
-
2
-
-
13
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
1
1
1
1
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
684567
Toyama
Membrane-bound, 2-keto-D-gluco ...
Gluconobacter frateurii, Gluconobacter frateurii IFO 3271
Appl. Environ. Microbiol.
73
6551-6556
2007
-
-
1
-
-
-
-
-
1
1
3
2
-
3
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
3
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685666
Saichana
Preparation of enzymes require ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Biosci. Biotechnol. Biochem.
71
2478-2486
2007
-
-
1
-
-
-
-
-
1
1
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
4
-
3
-
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286279
Yum
The yiaE gene, located at 80.1 ...
Escherichia coli
J. Bacteriol.
180
5984-5988
1998
-
-
1
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5693
Pitt
Enzymes of gluconate metabolis ...
Penicillium chrysogenum, Penicillium chrysogenum Westling
Antonie van Leeuwenhoek
51
353-364
1985
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286275
Ameyama
-
2-keto-D-Gluconate reductase f ...
Acetobacter ascendens, Acetobacter pasteurianus, Gluconacetobacter liquefaciens, Gluconobacter oxydans
Methods Enzymol.
89
203-210
1982
-
-
-
3
-
-
8
-
4
4
5
3
-
4
-
-
3
-
-
-
3
4
31
2
-
-
4
-
8
-
4
12
-
-
-
-
-
-
12
3
-
-
-
8
-
-
4
4
5
3
-
-
-
3
-
-
3
4
31
2
-
-
4
-
8
-
4
-
-
-
-
-
-
-
286274
Adachi
Crystalline 2-ketogluconate re ...
Acetobacter ascendens
Agric. Biol. Chem.
42
2057-2062
1978
-
-
-
1
-
3
1
4
1
1
2
-
-
1
-
-
1
-
-
-
1
1
7
1
1
1
3
-
2
2
-
2
-
-
-
-
-
-
2
1
-
3
-
1
-
4
1
1
2
-
-
-
-
1
-
-
1
1
7
1
1
1
3
-
2
2
-
-
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-
-
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-
286276
Shinagawa
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Distribution of gluconate dehy ...
acetic acid bacteria, no activity in a number of oxidative or aerobic bacteria
Agric. Biol. Chem.
42
1055-1057
1978
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1
-
2
-
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-
-
-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
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2
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286273
Chiyonobu
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Purification, crystallization ...
Acetobacter pasteurianus
Agric. Biol. Chem.
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175-184
1976
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1
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2
9
12
1
1
2
1
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1
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1
-
-
-
1
2
10
1
1
1
2
-
2
2
1
2
-
-
-
-
-
-
2
1
-
2
-
9
-
12
1
1
2
1
-
-
-
1
-
-
1
2
10
1
1
1
2
-
2
2
1
-
-
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