BRENDA - Enzyme Database show
show all sequences of 1.1.1.214

Purification and characterization of oxopantoyl lactone reductase from higher plants: role in pantothenate synthesis

Julliard, J.H.; Bot. Acta 107, 191-200 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-keto-4-hydroxybutyrolactone
strong and uncompetitive inhibitor
Spinacia oleracea
substituted 2,5-dioxopyrrolidines
strong and uncompetitive inhibitors
Spinacia oleracea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Spinacia oleracea
9507
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
36000
-
Spinacia oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ketopantoyl lactone + NADPH
Spinacia oleracea
-
pantoyl lactone + NADP+
the enzyme is involved in pantothenate biosynthesis, pantoyl lactone, the putative product of the reaction, together with beta-alanine and ATP are the substrate of pantothenate synthase
Spinacia oleracea
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
-
-
Purification (Commentary)
Commentary
Organism
using affinity chromatography on Red-Agarose and several subsequent ion exchange steps
Spinacia oleracea
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acenaphthenequinone
-
347731
Spinacia oleracea
?
-
347731
Spinacia oleracea
?
bornanedione + NADPH
-
347731
Spinacia oleracea
?
-
347731
Spinacia oleracea
?
isatin + NADPH
-
347731
Spinacia oleracea
?
-
347731
Spinacia oleracea
?
ketopantoyl lactone + NADPH
-
347731
Spinacia oleracea
pantoyl lactone + NADP+
-
347731
Spinacia oleracea
?
ketopantoyl lactone + NADPH
-
347731
Spinacia oleracea
pantoyl lactone + NADP+
the enzyme is involved in pantothenate biosynthesis, pantoyl lactone, the putative product of the reaction, together with beta-alanine and ATP are the substrate of pantothenate synthase
347731
Spinacia oleracea
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000-36000
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-keto-4-hydroxybutyrolactone
strong and uncompetitive inhibitor
Spinacia oleracea
substituted 2,5-dioxopyrrolidines
strong and uncompetitive inhibitors
Spinacia oleracea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Spinacia oleracea
9507
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
36000
-
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ketopantoyl lactone + NADPH
Spinacia oleracea
-
pantoyl lactone + NADP+
the enzyme is involved in pantothenate biosynthesis, pantoyl lactone, the putative product of the reaction, together with beta-alanine and ATP are the substrate of pantothenate synthase
Spinacia oleracea
?
Purification (Commentary) (protein specific)
Commentary
Organism
using affinity chromatography on Red-Agarose and several subsequent ion exchange steps
Spinacia oleracea
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acenaphthenequinone
-
347731
Spinacia oleracea
?
-
347731
Spinacia oleracea
?
bornanedione + NADPH
-
347731
Spinacia oleracea
?
-
347731
Spinacia oleracea
?
isatin + NADPH
-
347731
Spinacia oleracea
?
-
347731
Spinacia oleracea
?
ketopantoyl lactone + NADPH
-
347731
Spinacia oleracea
pantoyl lactone + NADP+
-
347731
Spinacia oleracea
?
ketopantoyl lactone + NADPH
-
347731
Spinacia oleracea
pantoyl lactone + NADP+
the enzyme is involved in pantothenate biosynthesis, pantoyl lactone, the putative product of the reaction, together with beta-alanine and ATP are the substrate of pantothenate synthase
347731
Spinacia oleracea
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000-36000
Spinacia oleracea
Other publictions for EC 1.1.1.214
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654311
Kataoka
Gene cloning and overexpressio ...
Candida parapsilosis, Candida parapsilosis IFO 0708
Appl. Microbiol. Biotechnol.
64
359-366
2004
-
1
1
-
-
-
1
-
-
-
2
2
-
10
-
-
-
-
-
-
2
-
4
1
1
-
-
-
1
1
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
2
2
-
-
-
-
-
-
2
-
4
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
347731
Julliard
-
Purification and characterizat ...
Spinacia oleracea
Bot. Acta
107
191-200
1994
-
-
-
-
-
-
2
-
1
-
1
1
-
1
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
1
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286105
Wilken
Ketopantoic acid and ketopanto ...
Escherichia coli
J. Biol. Chem.
250
2311-2314
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-