Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(4'nitrophenyl)prop-2-en-1-ol | inactivation dependent on NAD+ concentration, optimal at 0.5-1.0 mM NAD+, 2-mercaptoethanol prvides a concentration-dependent protection | Rattus norvegicus | |
1-(4'nitrophenyl)prop-2-en-1-one | inactivation can be retarded markedly in a concentration-dependent manner by both NADH and NADPH. Competitive inhibitor of NAD+ binding, measured for androsterone oxidation | Rattus norvegicus | |
1-(4'nitrophenyl)prop-2-yn-1-one | competitive inhibitor of NAD+ binding, measured for androsterone oxidation | Rattus norvegicus | |
17beta-bromoacetoxy-5alpha-dihydrotestosterone | inactivation by modification of steroid-binding site | Rattus norvegicus | |
testosterone | competitive inhibitor of androsterone binding | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male | - |
Rattus norvegicus | - |
Sprangue-Dawley | - |
Rattus norvegicus Sprangue-Dawley | - |
Sprangue-Dawley | - |
Purification (Comment) | Organism |
---|---|
CaCl2 and ammonium sulfate fractionation, DEAE-cellulose- and hydroxyapatite chromatography, chromatofocusing, Sephadex G-100 | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | ordered and sequential mechanism, nucleotide binds first and leaves last | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-80°C, 20 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 1 mM DTT, 30% glycerol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4'nitrophenyl)prop-2-en-1-ol + NAD+ | - |
Rattus norvegicus | 1-(4'nitrophenyl)prop-2-en-1-one + NADH | - |
? | |
1-(4'nitrophenyl)prop-2-en-1-ol + NAD+ | - |
Rattus norvegicus Sprangue-Dawley | 1-(4'nitrophenyl)prop-2-en-1-one + NADH | - |
? | |
1-(4'nitrophenyl)prop-2-yn-1-ol + NAD+ | - |
Rattus norvegicus | 1-(4'nitrophenyl)prop-2-yn-1-one + NADH | - |
? | |
1-(4'nitrophenyl)prop-2-yn-1-ol + NAD+ | - |
Rattus norvegicus Sprangue-Dawley | 1-(4'nitrophenyl)prop-2-yn-1-one + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus |