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Literature summary for 1.1.1.213 extracted from

  • Ricigliano, J.W.; Penning, T.M.
    Evidence that enzyme-generated aromatic Michael acceptors covalently modify the nucleotide-binding site of 3alpha-hydroxysteroid dehydrogenase (1990), Biochem. J., 269, 749-755.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1-(4'nitrophenyl)prop-2-en-1-ol inactivation dependent on NAD+ concentration, optimal at 0.5-1.0 mM NAD+, 2-mercaptoethanol prvides a concentration-dependent protection Rattus norvegicus
1-(4'nitrophenyl)prop-2-en-1-one inactivation can be retarded markedly in a concentration-dependent manner by both NADH and NADPH. Competitive inhibitor of NAD+ binding, measured for androsterone oxidation Rattus norvegicus
1-(4'nitrophenyl)prop-2-yn-1-one competitive inhibitor of NAD+ binding, measured for androsterone oxidation Rattus norvegicus
17beta-bromoacetoxy-5alpha-dihydrotestosterone inactivation by modification of steroid-binding site Rattus norvegicus
testosterone competitive inhibitor of androsterone binding Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male
-
Rattus norvegicus
-
Sprangue-Dawley
-
Rattus norvegicus Sprangue-Dawley
-
Sprangue-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
CaCl2 and ammonium sulfate fractionation, DEAE-cellulose- and hydroxyapatite chromatography, chromatofocusing, Sephadex G-100 Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ ordered and sequential mechanism, nucleotide binds first and leaves last Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-80°C, 20 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 1 mM DTT, 30% glycerol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(4'nitrophenyl)prop-2-en-1-ol + NAD+
-
Rattus norvegicus 1-(4'nitrophenyl)prop-2-en-1-one + NADH
-
?
1-(4'nitrophenyl)prop-2-en-1-ol + NAD+
-
Rattus norvegicus Sprangue-Dawley 1-(4'nitrophenyl)prop-2-en-1-one + NADH
-
?
1-(4'nitrophenyl)prop-2-yn-1-ol + NAD+
-
Rattus norvegicus 1-(4'nitrophenyl)prop-2-yn-1-one + NADH
-
?
1-(4'nitrophenyl)prop-2-yn-1-ol + NAD+
-
Rattus norvegicus Sprangue-Dawley 1-(4'nitrophenyl)prop-2-yn-1-one + NADH
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
NADP+
-
Rattus norvegicus