BRENDA - Enzyme Database show
show all sequences of 1.1.1.213

Prostaglandin dehydrogenase activity of purified rat liver 3alpha-hydroxysteroid dehydrogenase

Penning, T.M.; Sharp, R.B; Biochem. Biophys. Res. Commun. 148, 646-652 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
arachidonic acid
-
Rattus norvegicus
indomethacin
-
Rattus norvegicus
non-steroidal anti-inflamatory drug
-
Rattus norvegicus
Prostaglandin
A2alpha
Rattus norvegicus
prostaglandin A2alpha
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
Prostaglandin
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
-
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
CaCl2 and ammonium sulfate fractionation, DEAE-cellulose- and hydroxyapatite chromatography, chromatofocusing, Sephadex G-100
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0005
0.0053
for prostaglandins A1, A2, B1, B2, D2, E1, E2, F1, F2alpha at concentrations of 0.06 mM
Rattus norvegicus
0.0007
0.0012
for 15-keto-prostaglandins E2 and F2alpha at concentrations of 0.06 mM
Rattus norvegicus
2
-
for androsterone
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
oxidation of 3alpha-hydroxysteroids, secondary alcohols and trans-dihydrodiol proximate carcinogens derived from polycyclic aromatic hydrocarbons, reduction of quinones, aromatic aldehydes and ketones, 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase activity of enzyme
347980
Rattus norvegicus
?
-
-
-
-
prostaglandin + NADP+
types A1, A2, B1, B2, D2, E1, E2, F1, F2alpha and 15-keto-prostaglandinE2 and F2alpha
347980
Rattus norvegicus
? + NADPH
-
347980
Rattus norvegicus
-
prostaglandin-F2alpha + NADP+
-
347980
Rattus norvegicus
prostaglandin F2 + prostaglandin B2 + NADPH
-
347980
Rattus norvegicus
?
Subunits
Subunits
Commentary
Organism
monomer
-
Rattus norvegicus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
indomethacin
-
Rattus norvegicus
0.003
-
prostaglandin A2alpha
-
Rattus norvegicus
0.006
-
arachidonic acid
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arachidonic acid
-
Rattus norvegicus
indomethacin
-
Rattus norvegicus
non-steroidal anti-inflamatory drug
-
Rattus norvegicus
Prostaglandin
A2alpha
Rattus norvegicus
prostaglandin A2alpha
-
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
indomethacin
-
Rattus norvegicus
0.003
-
prostaglandin A2alpha
-
Rattus norvegicus
0.006
-
arachidonic acid
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
Prostaglandin
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
-
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
CaCl2 and ammonium sulfate fractionation, DEAE-cellulose- and hydroxyapatite chromatography, chromatofocusing, Sephadex G-100
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0005
0.0053
for prostaglandins A1, A2, B1, B2, D2, E1, E2, F1, F2alpha at concentrations of 0.06 mM
Rattus norvegicus
0.0007
0.0012
for 15-keto-prostaglandins E2 and F2alpha at concentrations of 0.06 mM
Rattus norvegicus
2
-
for androsterone
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
oxidation of 3alpha-hydroxysteroids, secondary alcohols and trans-dihydrodiol proximate carcinogens derived from polycyclic aromatic hydrocarbons, reduction of quinones, aromatic aldehydes and ketones, 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase activity of enzyme
347980
Rattus norvegicus
?
-
-
-
-
prostaglandin + NADP+
types A1, A2, B1, B2, D2, E1, E2, F1, F2alpha and 15-keto-prostaglandinE2 and F2alpha
347980
Rattus norvegicus
? + NADPH
-
347980
Rattus norvegicus
-
prostaglandin-F2alpha + NADP+
-
347980
Rattus norvegicus
prostaglandin F2 + prostaglandin B2 + NADPH
-
347980
Rattus norvegicus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Rattus norvegicus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
Other publictions for EC 1.1.1.213
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724719
Matsunaga
Pathophysiological roles of al ...
Homo sapiens
Chem. Biol. Interact.
202
234-242
2013
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1
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1
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1
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1
1
1
1
1
1
724915
Heinrich
Synthesis and structure-activi ...
Homo sapiens
Eur. J. Med. Chem.
62
738-744
2013
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3
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724916
Gazvoda
2,3-diarylpropenoic acids as s ...
Homo sapiens
Eur. J. Med. Chem.
62
89-97
2013
-
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1
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4
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723866
Jackson
Structure of AKR1C3 with 3-phe ...
Homo sapiens
Acta Crystallogr. Sect. F
68
409-413
2012
-
-
1
1
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1
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1
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724533
Chen
Crystal structures of AKR1C3 c ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
3492-3497
2012
-
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1
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2
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724538
Sinreih
N-Benzoyl anthranilic acid der ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
5948-5951
2012
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4
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725153
Miller
Aldo-keto reductase family 1 m ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
5
278-289
2012
-
1
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8
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1
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8
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725671
Brozic
Selective inhibitors of aldo-k ...
Homo sapiens
J. Med. Chem.
55
7417-7424
2012
-
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1
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2
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2
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1
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725825
Byrns
Overexpression of aldo-keto re ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
130
7-15
2012
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1
1
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724517
Adeniji
Discovery of substituted 3-(ph ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
1464-1468
2011
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3
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725152
Zakharov
Suppressed expression of type ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
3
608-617
2010
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1
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1
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1
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-
697265
Skarydova
AKR1C3 as a potential target f ...
Homo sapiens
Chem. Biol. Interact.
178
138-144
2009
-
1
1
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15
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2
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1
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7
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1
1
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7
15
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1
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685529
Uwai
Electronic effects of para-sub ...
Rattus norvegicus
Bioorg. Med. Chem.
16
1084-1089
2008
-
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1
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2
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2
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8
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1
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1
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1
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1
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2
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8
-
1
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1
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-
688137
Azzarello
Tissue distribution of human A ...
Homo sapiens, Rattus norvegicus
J. Histochem. Cytochem.
56
853-861
2008
-
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1
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6
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2
2
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7
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14
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2
1
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1
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6
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2
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14
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2
1
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689169
Meyer
The biological activity of 3al ...
Rattus norvegicus
Neurobiol. Dis.
30
30-41
2008
-
1
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-
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-
-
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1
1
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3
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2
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1
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2
1
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697310
Shultz
Fjord-region benzo[g]chrysene- ...
Rattus norvegicus
Chem. Res. Toxicol.
21
668-677
2008
-
-
1
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8
-
2
2
-
-
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3
-
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1
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2
1
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2
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1
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1
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1
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1
1
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8
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2
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2
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1
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2
1
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2
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1
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1
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-
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-
-
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-
684676
Sanai
Rat NAD+-dependent 3alpha-hydr ...
Rattus norvegicus
Arch. Biochem. Biophys.
464
122-129
2007
-
-
1
-
3
-
3
9
3
-
2
-
-
2
-
-
1
1
-
3
2
-
31
1
1
-
-
9
1
1
-
4
3
-
-
-
-
1
4
-
3
-
-
3
3
9
3
-
2
-
-
-
-
1
-
3
2
-
31
1
1
-
-
9
1
1
-
-
-
-
-
-
-
-
687635
Cooper
Elucidation of a complete kine ...
Rattus norvegicus
J. Biol. Chem.
282
33484-33493
2007
-
-
-
-
-
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3
-
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1
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2
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1
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2
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1
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1
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2
1
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2
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2
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3
-
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1
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2
-
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1
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1
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2
1
-
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-
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687639
McCarthy
3-ketosteroid reductase activi ...
Rattus norvegicus
J. Biol. Chem.
282
34003-34012
2007
1
-
1
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2
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2
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2
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3
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2
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1
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1
2
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687870
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Understanding oligomerization ...
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Multiplicity of mammalian redu ...
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672088
Jin
Multiple steps determine the o ...
Homo sapiens
Biochemistry
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673349
Papari-Zareei
Arginine 276 controls the dire ...
Rattus norvegicus
Endocrinology
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1591-1597
2006
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673967
Rani
Discrete analysis of bile acid ...
Comamonas testosteroni
Indian J. Biochem. Biophys.
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Apo- and holo-structures of 3a ...
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Okochi
Enhanced activity of 3alpha-hy ...
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Tibolone metabolism in human l ...
Homo sapiens
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Wiebe
Dutasteride affects progestero ...
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2006
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Isolation and characterization ...
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Bauman
Identification of the major ox ...
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2006
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Yee
Fluorogenic metabolic probes f ...
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Hwang
Mechanistic roles of Ser-114, ...
Comamonas testosteroni
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673101
Uwai
Purification and characterizat ...
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1
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677001
Couture
Comparison of crystal structur ...
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2005
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Heredia
Dissection of the physiologica ...
Rattus norvegicus
Biochemistry
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654756
Heredia
Alanine scanning mutagenesis o ...
Rattus norvegicus
Biochemistry
43
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2004
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Ma
Mutation of nicotinamide pocke ...
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Penning
Structure and function of 3alp ...
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3alpha-Hydroxysteroid oxidored ...
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Characterization of the substr ...
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Expression in Escherichia coli ...
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The kinetic mechanism catalyse ...
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Cloning and sequencing of the ...
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Demonstration of 3alpha(17beta ...
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Evidence that enzyme-generated ...
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Purification and properties of ...
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4
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5
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4
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