BRENDA - Enzyme Database show
show all sequences of 1.1.1.212

The characteristics of some components of the fatty acid synthetase system in the plastids from the mesocarp of avocado (Persea americana) fruit

Caughey, I.; Kekwick, R.G.O.; Eur. J. Biochem. 123, 553-561 (1982)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00273
-
NADH
-
Persea americana
0.00965
-
acetoacetyl-[acyl-carrier protein]
-
Persea americana
0.155
-
acetoacetyl-CoA
-
Persea americana
6.2
-
acetoacetyl N-acetyl-cysteamide
-
Persea americana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
168000
-
sucrose density gradient centrifugation
Persea americana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetoacetyl-[acyl-carrier protein] + NADH
Persea americana
component of fatty acid synthetase system
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
Persea americana
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Persea americana
-
avocado
-
Purification (Commentary)
Commentary
Organism
-
Persea americana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Persea americana
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0098
-
12000 x g precipitate of homogenate
Persea americana
Storage Stability
Storage Stability
Organism
-20░C, 50 mM phosphate buffer, pH 7.0, 10% glycerol, stable for two weeks
Persea americana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoacetyl-CoA + NADH
-
285678
Persea americana
3-hydroxybutanoyl-CoA + NAD+
-
-
-
?
acetoacetyl-N-acetylcysteamine + NADH
very poor substrate
285678
Persea americana
3-hydroxybutanoyl-N-acetylcysteamine + NAD+
-
-
-
?
acetoacetyl-[acyl-carrier protein] + NADH
-
285678
Persea americana
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
285678
Persea americana
?
acetoacetyl-[acyl-carrier protein] + NADH
component of fatty acid synthetase system
285678
Persea americana
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
285678
Persea americana
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Persea americana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
-
Persea americana
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Persea americana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Persea americana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00273
-
NADH
-
Persea americana
0.00965
-
acetoacetyl-[acyl-carrier protein]
-
Persea americana
0.155
-
acetoacetyl-CoA
-
Persea americana
6.2
-
acetoacetyl N-acetyl-cysteamide
-
Persea americana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
168000
-
sucrose density gradient centrifugation
Persea americana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetoacetyl-[acyl-carrier protein] + NADH
Persea americana
component of fatty acid synthetase system
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
Persea americana
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Persea americana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Persea americana
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0098
-
12000 x g precipitate of homogenate
Persea americana
Storage Stability (protein specific)
Storage Stability
Organism
-20░C, 50 mM phosphate buffer, pH 7.0, 10% glycerol, stable for two weeks
Persea americana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoacetyl-CoA + NADH
-
285678
Persea americana
3-hydroxybutanoyl-CoA + NAD+
-
-
-
?
acetoacetyl-N-acetylcysteamine + NADH
very poor substrate
285678
Persea americana
3-hydroxybutanoyl-N-acetylcysteamine + NAD+
-
-
-
?
acetoacetyl-[acyl-carrier protein] + NADH
-
285678
Persea americana
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
285678
Persea americana
?
acetoacetyl-[acyl-carrier protein] + NADH
component of fatty acid synthetase system
285678
Persea americana
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
285678
Persea americana
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Persea americana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
-
Persea americana
Other publictions for EC 1.1.1.212
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285678
Caughey
The characteristics of some co ...
Persea americana
Eur. J. Biochem.
123
553-561
1982
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4
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1
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1
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1
4
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1
1
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1
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1
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4
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1
1
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1
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1
1
1
4
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1
1
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