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Literature summary for 1.1.1.210 extracted from
- Isolation of 3beta,20alpha-hydroxysteroid oxidoreductase from sheep fetal blood (1987), Steroids, 49, 477-496.
General Stability
| General Stability | Organism |
|---|---|
| stable to bromoacetic acid | Ovis aries |
| stable to ethyl bromoacetate | Ovis aries |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 16alpha-(Bromoacetoxy)progesterone | competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation, 2-mercaptoethanol protects | Ovis aries |  |
| 17beta-hydroxy-5alpha-androstan-3-one | inhibition of 20alpha-reductase activity | Ovis aries | |
| progesterone | inhibition of 3beta-reductase activity | Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
progesterone | - |
Ovis aries | |
| 0.074 | - |
17beta-hydroxy-5alpha-androstan-3-one | - |
Ovis aries | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Ovis aries | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Ovis aries |
| 36000 | - |
1 * 36000, SDS-PAGE | Ovis aries |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-hydroxy-5alpha-androstan-3-one + NADPH | Ovis aries | - |
5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? | |
| progesterone + NADPH | Ovis aries | - |
20alpha-hydroxy-4-pregnen-3-one + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+ | 3beta and 20alpha substrates share the same active site | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | fetal | Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0071 | - |
20alpha-reductase activity | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-hydoxy-5alpha-androstan-3-one + NADPH | - |
Ovis aries | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? | |
| 17beta-hydroxy-5alpha-androstan-3-one + NADPH | - |
Ovis aries | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? | |
| progesterone + NADPH | - |
Ovis aries | 20alpha-hydroxy-4-pregnen-3-one + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Ovis aries | |
| NADPH | - |
Ovis aries | |
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Release 2023.1 (January 2023)
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