BRENDA - Enzyme Database show
show all sequences of 1.1.1.210

Isolation of 3beta,20alpha-hydroxysteroid oxidoreductase from sheep fetal blood

Chen, Q.; Nancarrow, C.D.; Sweet, F.; Steroids 49, 477-496 (1987)

Data extracted from this reference:

General Stability
General Stability
Organism
stable to bromoacetic acid
Ovis aries
stable to ethyl bromoacetate
Ovis aries
Inhibitors
Inhibitors
Commentary
Organism
Structure
16alpha-(Bromoacetoxy)progesterone
competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation, 2-mercaptoethanol protects
Ovis aries
17beta-hydroxy-5alpha-androstan-3-one
inhibition of 20alpha-reductase activity
Ovis aries
progesterone
inhibition of 3beta-reductase activity
Ovis aries
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
progesterone
-
Ovis aries
0.074
-
17beta-hydroxy-5alpha-androstan-3-one
-
Ovis aries
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Ovis aries
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Ovis aries
36000
-
1 * 36000, SDS-PAGE
Ovis aries
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
17beta-hydroxy-5alpha-androstan-3-one + NADPH
Ovis aries
-
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
?
progesterone + NADPH
Ovis aries
-
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries
-
-
-
Purification (Commentary)
Commentary
Organism
-
Ovis aries
Reaction
Reaction
Commentary
Organism
5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+
3beta and 20alpha substrates share the same active site
Ovis aries
Source Tissue
Source Tissue
Commentary
Organism
Textmining
erythrocyte
fetal
Ovis aries
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0071
-
20alpha-reductase activity
Ovis aries
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydoxy-5alpha-androstan-3-one + NADPH
-
286266
Ovis aries
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
286266
Ovis aries
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
progesterone + NADPH
-
286266
Ovis aries
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Ovis aries
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Ovis aries
NADPH
-
Ovis aries
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Ovis aries
NADPH
-
Ovis aries
General Stability (protein specific)
General Stability
Organism
stable to bromoacetic acid
Ovis aries
stable to ethyl bromoacetate
Ovis aries
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
16alpha-(Bromoacetoxy)progesterone
competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation, 2-mercaptoethanol protects
Ovis aries
17beta-hydroxy-5alpha-androstan-3-one
inhibition of 20alpha-reductase activity
Ovis aries
progesterone
inhibition of 3beta-reductase activity
Ovis aries
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
progesterone
-
Ovis aries
0.074
-
17beta-hydroxy-5alpha-androstan-3-one
-
Ovis aries
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Ovis aries
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Ovis aries
36000
-
1 * 36000, SDS-PAGE
Ovis aries
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
17beta-hydroxy-5alpha-androstan-3-one + NADPH
Ovis aries
-
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
?
progesterone + NADPH
Ovis aries
-
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ovis aries
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
erythrocyte
fetal
Ovis aries
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0071
-
20alpha-reductase activity
Ovis aries
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydoxy-5alpha-androstan-3-one + NADPH
-
286266
Ovis aries
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
286266
Ovis aries
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
progesterone + NADPH
-
286266
Ovis aries
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Ovis aries
Other publictions for EC 1.1.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
657329
Clementi
Luteal 3beta-hydroxysteroid de ...
Rattus norvegicus
Reprod. Biol. Endocrinol.
2
22
2004
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286268
Chen
-
Identification of histidine re ...
Ovis aries
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan
18
113-117
1991
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286267
Chen
Fetal lamb 3beta,20alpha-hydro ...
Ovis aries
Biochemistry
28
8856-8863
1989
-
-
-
-
-
-
-
1
1
-
2
-
-
1
-
-
1
1
-
1
1
1
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
2
-
-
-
-
1
-
1
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286266
Chen
Isolation of 3beta,20alpha-hyd ...
Ovis aries
Steroids
49
477-496
1987
-
-
-
-
-
2
3
2
1
-
2
2
-
1
-
-
1
1
-
1
1
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
-
3
-
2
1
-
2
2
-
-
-
1
-
1
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286265
Sharaf
Dual activity at an enzyme act ...
Bos taurus
Biochemistry
21
4615-4620
1982
-
-
-
-
-
1
3
2
1
-
1
2
-
1
-
-
1
1
-
1
-
1
3
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
3
-
2
1
-
1
2
-
-
-
1
-
1
-
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-