BRENDA - Enzyme Database show
show all sequences of 1.1.1.210

Dual activity at an enzyme active site: 3beta,20alpha-hydroxysteroid oxidoreductase from fetal blood

Sharaf, M.A.; Sweet, F.; Biochemistry 21, 4615-4620 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
lyophilization, complete loss of activity
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
17beta-hydroxy-5alpha-androstan-3-one
inhibition of 20alpha-reductase activity
Bos taurus
19-Nortestosterone 17-bromoacetate
competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation
Bos taurus
progesterone
inhibition of 3beta-reductase activity
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0025
-
NADPH
20alpha-reductase
Bos taurus
0.0094
-
NADPH
3beta-reductase
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Bos taurus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
SDS-PAGE, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
17beta-hydroxy-5alpha-androstan-3-one + NADPH
Bos taurus
-
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
?
progesterone + NADPH
Bos taurus
-
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Reaction
Reaction
Commentary
Organism
5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+
3beta and 20alpha substrates share the same active site
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
erythrocyte
fetal
Bos taurus
-
Storage Stability
Storage Stability
Organism
-20°C, purified enzyme, indefinitely stable
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydoxy-5alpha-androstan-3-one + NADPH
-
286265
Bos taurus
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
286265
Bos taurus
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
progesterone + NADPH
-
286265
Bos taurus
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 55000, SDS-PAGE
Bos taurus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Bos taurus
NADPH
-
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Bos taurus
NADPH
-
Bos taurus
General Stability (protein specific)
General Stability
Organism
lyophilization, complete loss of activity
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
17beta-hydroxy-5alpha-androstan-3-one
inhibition of 20alpha-reductase activity
Bos taurus
19-Nortestosterone 17-bromoacetate
competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation
Bos taurus
progesterone
inhibition of 3beta-reductase activity
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0025
-
NADPH
20alpha-reductase
Bos taurus
0.0094
-
NADPH
3beta-reductase
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Bos taurus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
SDS-PAGE, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
17beta-hydroxy-5alpha-androstan-3-one + NADPH
Bos taurus
-
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
?
progesterone + NADPH
Bos taurus
-
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
erythrocyte
fetal
Bos taurus
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, purified enzyme, indefinitely stable
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydoxy-5alpha-androstan-3-one + NADPH
-
286265
Bos taurus
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
286265
Bos taurus
5alpha-androstan-3beta,17beta-diol + NADP+
-
-
-
?
progesterone + NADPH
-
286265
Bos taurus
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 55000, SDS-PAGE
Bos taurus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Bos taurus
Other publictions for EC 1.1.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
657329
Clementi
Luteal 3beta-hydroxysteroid de ...
Rattus norvegicus
Reprod. Biol. Endocrinol.
2
22
2004
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286268
Chen
-
Identification of histidine re ...
Ovis aries
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan
18
113-117
1991
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286267
Chen
Fetal lamb 3beta,20alpha-hydro ...
Ovis aries
Biochemistry
28
8856-8863
1989
-
-
-
-
-
-
-
1
1
-
2
-
-
1
-
-
1
1
-
1
1
1
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
2
-
-
-
-
1
-
1
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286266
Chen
Isolation of 3beta,20alpha-hyd ...
Ovis aries
Steroids
49
477-496
1987
-
-
-
-
-
2
3
2
1
-
2
2
-
1
-
-
1
1
-
1
1
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
-
3
-
2
1
-
2
2
-
-
-
1
-
1
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286265
Sharaf
Dual activity at an enzyme act ...
Bos taurus
Biochemistry
21
4615-4620
1982
-
-
-
-
-
1
3
2
1
-
1
2
-
1
-
-
1
1
-
1
-
1
3
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
3
-
2
1
-
1
2
-
-
-
1
-
1
-
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-