General Stability | Organism |
---|---|
lyophilization, complete loss of activity | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one | inhibition of 20alpha-reductase activity | Bos taurus | |
19-Nortestosterone 17-bromoacetate | competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation | Bos taurus | |
progesterone | inhibition of 3beta-reductase activity | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
NADPH | 20alpha-reductase | Bos taurus | |
0.0094 | - |
NADPH | 3beta-reductase | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE, gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one + NADPH | Bos taurus | - |
5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? | |
progesterone + NADPH | Bos taurus | - |
20alpha-hydroxy-4-pregnen-3-one + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+ | 3beta and 20alpha substrates share the same active site | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | fetal | Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, indefinitely stable | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydoxy-5alpha-androstan-3-one + NADPH | - |
Bos taurus | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? | |
17beta-hydroxy-5alpha-androstan-3-one + NADPH | - |
Bos taurus | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? | |
progesterone + NADPH | - |
Bos taurus | 20alpha-hydroxy-4-pregnen-3-one + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bos taurus | |
NADPH | - |
Bos taurus |