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Literature summary for 1.1.1.21 extracted from

  • Rath, J.; Gowri, V.S.; Chauhan, S.C.; Padmanabhan, P.K.; Srinivasan, N.; Madhubala, R.
    A glutathione-specific aldose reductase of Leishmania donovani and its potential implications for methylglyoxal detoxification pathway (2009), Gene, 429, 1-9.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glutathione ALR shows a high level of specificity towards glutathione conjugates and very little specificity towards trypanothione conjugates Leishmania donovani

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli cells Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.112
-
methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM glutathione Leishmania donovani
0.4
-
methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM trypanothione Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31700
-
subunit, calculated from amino acid sequence Leishmania donovani
74000
-
recombinant enzyme with a maltose binding tag, SDS-PAGE Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
isolate 2001-S
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania donovani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetol + NADPH
-
Leishmania donovani propane-1,2 diol + NADP+
-
?
methylglyoxal + NADPH + H+
-
Leishmania donovani acetol + NADP+
-
?

Synonyms

Synonyms Comment Organism
aldose reductase
-
Leishmania donovani
ALR
-
Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44.4
-
methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM trypanothione Leishmania donovani
78.4
-
methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM glutathione Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Leishmania donovani

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania donovani calculated from amino acid sequence
-
5.85