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show all sequences of 1.1.1.209

Studies on a Tyr residue critical for the binding of coenzyme and substrate in mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21): structure of the Y224D mutant enzyme

Dhagat, U.; Endo, S.; Mamiya, H.; Hara, A.; El-Kabbani, O.; Acta Crystallogr. Sect. D 66, 198-204 (2010)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
Y224D mutant at 2.3 A resolution. Mutation results in a change in the conformation of the flexible loop B, including the V-shaped groove
Mus musculus
Engineering
Amino acid exchange
Commentary
Organism
Q222N
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type
Mus musculus
Y224D
mutation reduces the Km value for NADP(H) by up to 80fold and completely reverses the 17alpha stereospecificity of the enzyme
Mus musculus
Y224F
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
lithocholic acid
wild-type, pH 7.0, 25°C
Mus musculus
0.0002
-
4-androstene-3,17-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.0002
-
5beta-pregnane-20alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.0002
-
lithocholic acid
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0004
-
5alpha-Androstan-17alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.0004
-
5beta-pregnane-3,20-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0005
-
5beta-pregnane-3,20-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0006
-
5alpha-Androstan-3alpha-ol-17-one
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0006
-
5beta-pregnane-3,20-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
5beta-pregnane-3alpha,20alpha-diol
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
Epitestosterone
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
lithocholic acid
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0007
-
5beta-pregnane-20alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0015
-
4-androstene-3,17-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0018
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0032
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0034
-
5alpha-Androstan-3alpha-ol-17-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0038
-
5beta-pregnane-3alpha,20alpha-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0047
-
5alpha-androstan-17beta-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0054
-
5alpha-Androstan-17alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0059
-
4-androstene-3,17-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0059
-
5beta-pregnane-3,20-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0067
-
lithocholic acid
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0078
-
4-androstene-3,17-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.013
-
5alpha-androstan-3beta,17beta-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.014
-
5alpha-Androstan-17alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.014
-
Epitestosterone
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.015
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.015
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.018
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.02
-
Epitestosterone
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.035
-
5alpha-androstan-3beta,17beta-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.08
-
5alpha-androstan-17beta-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.096
-
5alpha-androstan-17beta-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.1
-
5alpha-androstan-3beta,17beta-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q91WR5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androstene-3,17-dione + NADPH + H+
-
710716
Mus musculus
? + NADP+
-
-
-
r
5-androstene-3beta,17beta-diol + NADP+
no substrate for wild-type, but substrate for mutants Y224F, Y224D, Q222D
710716
Mus musculus
? + NADPH
-
-
-
?
5alpha-androstan-17alpha-ol-3-one + NADP+
-
710716
Mus musculus
5alpha-androstan-3,17-dione + NADPH + H+
-
-
-
?
5alpha-androstan-17beta-ol-3-one + NADP+
-
710716
Mus musculus
5alpha-androstan-3,17-dione + NADPH + H+
no substrate for wild-type, but substrate for mutants Y224F, Y224D, Q222D
-
-
?
5alpha-androstan-3alpha-ol-17-one + NADPH + H+
-
710716
Mus musculus
5alpha-androstan-3alpha-ol-17beta-ol + NADP+
-
-
-
?
5alpha-androstan-3beta,17beta-diol + NADP+
-
710716
Mus musculus
? + NADPH
-
-
-
?
5alpha-androstan-3beta,17beta-diol + NADP+
-
710716
Mus musculus
? + NADPH + H+
-
-
-
?
5beta-pregnane-20alpha-ol-3-one + NADP+
-
710716
Mus musculus
5beta-pregnane-3alpha,20alpha-diol + NADPH + H+
-
-
-
r
5beta-pregnane-3,20-dione + NADPH + H+
-
710716
Mus musculus
5beta-pregnane-3alpha-ol-20-one + NADP+
-
-
-
r
5beta-pregnane-3alpha,20alpha-diol + NADPH + H+
-
710716
Mus musculus
5beta-pregnane-20alpha-ol-3-one + NADP+
-
-
-
r
epitestosterone + NADP+
-
710716
Mus musculus
testosterone + NADPH + H+
-
-
-
?
lithocholic acid + NADP+
-
710716
Mus musculus
? + NADPH + H+
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00167
-
5alpha-androstan-3beta,17beta-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.00217
-
5alpha-androstan-3beta,17beta-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0025
-
5alpha-androstan-17beta-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0027
-
5alpha-androstan-17beta-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.00367
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0073
-
lithocholic acid
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0217
-
4-androstene-3,17-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0217
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0367
-
5alpha-androstan-17beta-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.047
-
lithocholic acid
wild-type, pH 7.0, 25°C
Mus musculus
0.065
-
5alpha-androstan-3beta,17beta-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.073
-
5alpha-Androstan-3alpha-ol-17-one
wild-type, pH 7.0, 25°C
Mus musculus
0.081
-
lithocholic acid
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.105
-
lithocholic acid
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.127
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.167
-
4-androstene-3,17-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-Androstan-17alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-androstan-17beta-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-androstan-3beta,17beta-diol
wild-type, pH 7.0, 25°C
Mus musculus
0.217
-
5beta-pregnane-3alpha,20alpha-diol
wild-type, pH 7.0, 25°C
Mus musculus
0.23
-
Epitestosterone
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.233
-
5alpha-Androstan-3alpha-ol-17-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.25
-
5beta-pregnane-3alpha,20alpha-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.283
-
5alpha-Androstan-17alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.3
-
Epitestosterone
wild-type, pH 7.0, 25°C
Mus musculus
0.317
-
4-androstene-3,17-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.35
-
5beta-pregnane-3,20-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.383
-
5beta-pregnane-20alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.4
-
5beta-pregnane-3,20-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.4
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.57
-
Epitestosterone
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.617
-
4-androstene-3,17-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.867
-
5alpha-Androstan-17alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
1.25
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
2.83
-
5beta-pregnane-20alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
4
-
5beta-pregnane-3,20-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
4.83
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
4.83
-
5beta-pregnane-3,20-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
Y224D mutant at 2.3 A resolution. Mutation results in a change in the conformation of the flexible loop B, including the V-shaped groove
Mus musculus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Q222N
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type
Mus musculus
Y224D
mutation reduces the Km value for NADP(H) by up to 80fold and completely reverses the 17alpha stereospecificity of the enzyme
Mus musculus
Y224F
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
lithocholic acid
wild-type, pH 7.0, 25°C
Mus musculus
0.0002
-
4-androstene-3,17-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.0002
-
5beta-pregnane-20alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.0002
-
lithocholic acid
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0004
-
5alpha-Androstan-17alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.0004
-
5beta-pregnane-3,20-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0005
-
5beta-pregnane-3,20-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0006
-
5alpha-Androstan-3alpha-ol-17-one
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0006
-
5beta-pregnane-3,20-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
5beta-pregnane-3alpha,20alpha-diol
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
Epitestosterone
wild-type, pH 7.0, 25°C
Mus musculus
0.0006
-
lithocholic acid
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0007
-
5beta-pregnane-20alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0015
-
4-androstene-3,17-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0018
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0032
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0034
-
5alpha-Androstan-3alpha-ol-17-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0038
-
5beta-pregnane-3alpha,20alpha-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0047
-
5alpha-androstan-17beta-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0054
-
5alpha-Androstan-17alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0059
-
4-androstene-3,17-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.0059
-
5beta-pregnane-3,20-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0067
-
lithocholic acid
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0078
-
4-androstene-3,17-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.013
-
5alpha-androstan-3beta,17beta-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.014
-
5alpha-Androstan-17alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.014
-
Epitestosterone
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.015
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.015
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.018
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.02
-
Epitestosterone
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.035
-
5alpha-androstan-3beta,17beta-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.08
-
5alpha-androstan-17beta-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.096
-
5alpha-androstan-17beta-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.1
-
5alpha-androstan-3beta,17beta-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androstene-3,17-dione + NADPH + H+
-
710716
Mus musculus
? + NADP+
-
-
-
r
5-androstene-3beta,17beta-diol + NADP+
no substrate for wild-type, but substrate for mutants Y224F, Y224D, Q222D
710716
Mus musculus
? + NADPH
-
-
-
?
5alpha-androstan-17alpha-ol-3-one + NADP+
-
710716
Mus musculus
5alpha-androstan-3,17-dione + NADPH + H+
-
-
-
?
5alpha-androstan-17beta-ol-3-one + NADP+
-
710716
Mus musculus
5alpha-androstan-3,17-dione + NADPH + H+
no substrate for wild-type, but substrate for mutants Y224F, Y224D, Q222D
-
-
?
5alpha-androstan-3alpha-ol-17-one + NADPH + H+
-
710716
Mus musculus
5alpha-androstan-3alpha-ol-17beta-ol + NADP+
-
-
-
?
5alpha-androstan-3beta,17beta-diol + NADP+
-
710716
Mus musculus
? + NADPH
-
-
-
?
5alpha-androstan-3beta,17beta-diol + NADP+
-
710716
Mus musculus
? + NADPH + H+
-
-
-
?
5beta-pregnane-20alpha-ol-3-one + NADP+
-
710716
Mus musculus
5beta-pregnane-3alpha,20alpha-diol + NADPH + H+
-
-
-
r
5beta-pregnane-3,20-dione + NADPH + H+
-
710716
Mus musculus
5beta-pregnane-3alpha-ol-20-one + NADP+
-
-
-
r
5beta-pregnane-3alpha,20alpha-diol + NADPH + H+
-
710716
Mus musculus
5beta-pregnane-20alpha-ol-3-one + NADP+
-
-
-
r
epitestosterone + NADP+
-
710716
Mus musculus
testosterone + NADPH + H+
-
-
-
?
lithocholic acid + NADP+
-
710716
Mus musculus
? + NADPH + H+
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00167
-
5alpha-androstan-3beta,17beta-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.00217
-
5alpha-androstan-3beta,17beta-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.0025
-
5alpha-androstan-17beta-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0027
-
5alpha-androstan-17beta-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.00367
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0073
-
lithocholic acid
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0217
-
4-androstene-3,17-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0217
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.0367
-
5alpha-androstan-17beta-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.047
-
lithocholic acid
wild-type, pH 7.0, 25°C
Mus musculus
0.065
-
5alpha-androstan-3beta,17beta-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.073
-
5alpha-Androstan-3alpha-ol-17-one
wild-type, pH 7.0, 25°C
Mus musculus
0.081
-
lithocholic acid
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.105
-
lithocholic acid
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.127
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.167
-
4-androstene-3,17-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-Androstan-17alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-androstan-17beta-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-androstan-3beta,17beta-diol
wild-type, pH 7.0, 25°C
Mus musculus
0.217
-
5beta-pregnane-3alpha,20alpha-diol
wild-type, pH 7.0, 25°C
Mus musculus
0.23
-
Epitestosterone
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.233
-
5alpha-Androstan-3alpha-ol-17-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.25
-
5beta-pregnane-3alpha,20alpha-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.283
-
5alpha-Androstan-17alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.3
-
Epitestosterone
wild-type, pH 7.0, 25°C
Mus musculus
0.317
-
4-androstene-3,17-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.35
-
5beta-pregnane-3,20-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.383
-
5beta-pregnane-20alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
0.4
-
5beta-pregnane-3,20-dione
wild-type, pH 7.0, 25°C
Mus musculus
0.4
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.57
-
Epitestosterone
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.617
-
4-androstene-3,17-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
0.867
-
5alpha-Androstan-17alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
1.25
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
2.83
-
5beta-pregnane-20alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
4
-
5beta-pregnane-3,20-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
4.83
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
4.83
-
5beta-pregnane-3,20-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.03
-
5alpha-androstan-17beta-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.05
-
5alpha-androstan-3beta,17beta-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-androstan-3beta,17beta-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.5
-
5alpha-androstan-17beta-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.65
-
5alpha-androstan-3beta,17beta-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
1
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
1
-
lithocholic acid
mutant Y224D, pH 7.0, 25°C
Mus musculus
1.5
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
3.3
-
4-androstene-3,17-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
4.5
-
5alpha-androstan-17beta-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
11.67
-
Epitestosterone
mutant Q222N, pH 7.0, 25°C
Mus musculus
20
-
5alpha-Androstan-17alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
40
-
Epitestosterone
mutant Y224F, pH 7.0, 25°C
Mus musculus
60
-
5beta-pregnane-3,20-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
65
-
5beta-pregnane-3alpha,20alpha-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
68.3
-
5alpha-Androstan-3alpha-ol-17-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
70
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
83
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
105
-
4-androstene-3,17-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
122
-
5alpha-Androstan-3alpha-ol-17-one
wild-type, pH 7.0, 25°C
Mus musculus
125
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
160
-
5alpha-Androstan-17alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
183
-
lithocholic acid
mutant Q222N, pH 7.0, 25°C
Mus musculus
217
-
4-androstene-3,17-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
366
-
5beta-pregnane-3alpha,20alpha-diol
wild-type, pH 7.0, 25°C
Mus musculus
400
-
5beta-pregnane-20alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
400
-
lithocholic acid
mutant Y224F, pH 7.0, 25°C
Mus musculus
417
-
5alpha-Androstan-17alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
467
-
lithocholic acid
wild-type, pH 7.0, 25°C
Mus musculus
500
-
Epitestosterone
wild-type, pH 7.0, 25°C
Mus musculus
667
-
5beta-pregnane-3,20-dione
wild-type, pH 7.0, 25°C
Mus musculus
833
-
4-androstene-3,17-dione
wild-type, pH 7.0, 25°C
Mus musculus
1200
-
5beta-pregnane-3,20-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
2000
-
5beta-pregnane-20alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
8000
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
8000
-
5beta-pregnane-3,20-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.03
-
5alpha-androstan-17beta-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.05
-
5alpha-androstan-3beta,17beta-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
0.167
-
5alpha-androstan-3beta,17beta-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.5
-
5alpha-androstan-17beta-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
0.65
-
5alpha-androstan-3beta,17beta-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
1
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224D, pH 7.0, 25°C
Mus musculus
1
-
lithocholic acid
mutant Y224D, pH 7.0, 25°C
Mus musculus
1.5
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
3.3
-
4-androstene-3,17-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
4.5
-
5alpha-androstan-17beta-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
11.67
-
Epitestosterone
mutant Q222N, pH 7.0, 25°C
Mus musculus
20
-
5alpha-Androstan-17alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
40
-
Epitestosterone
mutant Y224F, pH 7.0, 25°C
Mus musculus
60
-
5beta-pregnane-3,20-dione
mutant Y224D, pH 7.0, 25°C
Mus musculus
65
-
5beta-pregnane-3alpha,20alpha-diol
mutant Q222N, pH 7.0, 25°C
Mus musculus
68.3
-
5alpha-Androstan-3alpha-ol-17-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
70
-
5alpha-Androstan-3alpha-ol-17-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
83
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224D, pH 7.0, 25°C
Mus musculus
105
-
4-androstene-3,17-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
122
-
5alpha-Androstan-3alpha-ol-17-one
wild-type, pH 7.0, 25°C
Mus musculus
125
-
5beta-pregnane-3alpha,20alpha-diol
mutant Y224F, pH 7.0, 25°C
Mus musculus
160
-
5alpha-Androstan-17alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
183
-
lithocholic acid
mutant Q222N, pH 7.0, 25°C
Mus musculus
217
-
4-androstene-3,17-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
366
-
5beta-pregnane-3alpha,20alpha-diol
wild-type, pH 7.0, 25°C
Mus musculus
400
-
5beta-pregnane-20alpha-ol-3-one
mutant Q222N, pH 7.0, 25°C
Mus musculus
400
-
lithocholic acid
mutant Y224F, pH 7.0, 25°C
Mus musculus
417
-
5alpha-Androstan-17alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
467
-
lithocholic acid
wild-type, pH 7.0, 25°C
Mus musculus
500
-
Epitestosterone
wild-type, pH 7.0, 25°C
Mus musculus
667
-
5beta-pregnane-3,20-dione
wild-type, pH 7.0, 25°C
Mus musculus
833
-
4-androstene-3,17-dione
wild-type, pH 7.0, 25°C
Mus musculus
1200
-
5beta-pregnane-3,20-dione
mutant Q222N, pH 7.0, 25°C
Mus musculus
2000
-
5beta-pregnane-20alpha-ol-3-one
wild-type, pH 7.0, 25°C
Mus musculus
8000
-
5beta-pregnane-20alpha-ol-3-one
mutant Y224F, pH 7.0, 25°C
Mus musculus
8000
-
5beta-pregnane-3,20-dione
mutant Y224F, pH 7.0, 25°C
Mus musculus
Other publictions for EC 1.1.1.209
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710716
Dhagat
Studies on a Tyr residue criti ...
Mus musculus
Acta Crystallogr. Sect. D
66
198-204
2010
-
-
-
1
3
-
-
36
-
-
-
-
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
38
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
36
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
38
-
-
-
-
-
-
-
-
36
36
695325
Dhagat
Structure of the G225P/G226P m ...
Mus musculus
Acta Crystallogr. Sect. D
65
257-265
2009
-
-
1
1
1
-
1
16
-
-
-
-
-
2
-
-
1
-
-
-
-
-
10
-
-
-
-
16
-
-
-
1
-
-
2
-
-
1
1
1
1
-
2
1
-
16
-
-
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
685538
Dhagat
Inhibition of 3(17)alpha-hydro ...
Mus musculus
Bioorg. Med. Chem.
16
3245-3254
2008
-
-
-
-
-
-
10
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
9
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684185
Dhagat
Structure of 3(17)alpha-hydrox ...
Mus musculus
Acta Crystallogr. Sect. F
63
825-830
2007
-
-
-
1
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667072
El-Kabbani
Crystallization and preliminar ...
Mus musculus
Acta crystallogr. Sect. F
61
688-690
2005
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389547
Lin
Expression and characterizatio ...
Homo sapiens
Mol. Endocrinol.
11
1971-1984
1997
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
5
3
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
5
3
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
286234
Nakagawa
Identification of two dihydrod ...
Mus musculus
J. Biochem.
106
633-638
1989
-
-
-
-
-
-
10
13
-
-
2
-
-
3
-
-
1
-
-
1
1
-
27
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
13
-
-
2
-
-
-
-
1
-
1
1
-
27
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286232
Lau
A 3(17) alpha-hydroxysteroid d ...
Oryctolagus cuniculus
J. Biol. Chem.
257
9444-9449
1982
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
1
5
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
-
1
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286233
Lau
Comparison of the multiple for ...
Oryctolagus cuniculus
J. Biol. Chem.
257
9450-9456
1982
-
-
-
-
-
-
1
6
1
-
-
-
-
1
-
-
-
-
-
2
-
-
6
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
6
1
-
-
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-