Literature summary for 1.1.1.209 extracted from

Lin, H.K.; Jez, J.M.; Schlegel, B.P.; Peehl, D.M.; Pachter, J.A.; Penning, T.M.
Expression and characterization of recombinant type 2: 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha/17beta-HSD activity and cellular distribution (1997), Mol. Endocrinol., 11, 1971-1984.

Search for more literature for 1.1.1.209

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	testosterone	-	Homo sapiens
0.019	-	(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide	-	Homo sapiens
0.026	-	5alpha-dihydrotestosterone	-	Homo sapiens
0.048	-	androsterone	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-
liver	-	Homo sapiens	-
placenta	-	Homo sapiens	-
prostate gland	-	Homo sapiens	-
spleen	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0035	-	reduction of 5alpha-dihydrotestosteerone, cofactor NADPH, pH 6.0	Homo sapiens
0.0063	-	oxidation of 3alpha-androstanediol, cofactor NADP+, pH 9.0	Homo sapiens
0.0253	-	reduction of 5alpha-androstanedione, cofactor NADPH, pH 6.0	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide + NADPH + H+	i.e., 5alpha-dihydrotestosterone	Homo sapiens	3alpha-androstanediol + NADP+	-	?
3alpha-androstanediol + NAD(P)+	-	Homo sapiens	androsterone + NAD(P)H	-	r
5alpha-dihydrotestosterone + NAD(P)H	-	Homo sapiens	androstanedione + NAD(P)+	-	r
testosterone + NAD(P)+	-	Homo sapiens	androstenedione + NAD(P)H + H+	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000367	-	androsterone	-	Homo sapiens
0.0009	-	testosterone	-	Homo sapiens
0.003	-	5alpha-dihydrotestosterone	-	Homo sapiens
0.00617	-	(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)