BRENDA - Enzyme Database show
show all sequences of 1.1.1.209

Expression and characterization of recombinant type 2: 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha/17beta-HSD activity and cellular distribution

Lin, H.K.; Jez, J.M.; Schlegel, B.P.; Peehl, D.M.; Pachter, J.A.; Penning, T.M.; Mol. Endocrinol. 11, 1971-1984 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
testosterone
-
Homo sapiens
0.019
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
Homo sapiens
0.026
-
5alpha-dihydrotestosterone
-
Homo sapiens
0.048
-
androsterone
-
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
liver
-
Homo sapiens
-
placenta
-
Homo sapiens
-
prostate gland
-
Homo sapiens
-
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0035
-
reduction of 5alpha-dihydrotestosteerone, cofactor NADPH, pH 6.0
Homo sapiens
0.0063
-
oxidation of 3alpha-androstanediol, cofactor NADP+, pH 9.0
Homo sapiens
0.0253
-
reduction of 5alpha-androstanedione, cofactor NADPH, pH 6.0
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha-androstanediol + NAD(P)+
-
389547
Homo sapiens
androsterone + NAD(P)H
-
389547
Homo sapiens
r
5alpha-dihydrotestosterone + NAD(P)H
-
389547
Homo sapiens
androstanedione + NAD(P)+
-
389547
Homo sapiens
r
testosterone + NAD(P)+
-
389547
Homo sapiens
androstenedione + NAD(P)H + H+
-
389547
Homo sapiens
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000367
-
androsterone
-
Homo sapiens
0.0009
-
testosterone
-
Homo sapiens
0.003
-
5alpha-dihydrotestosterone
-
Homo sapiens
0.00617
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
testosterone
-
Homo sapiens
0.019
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
Homo sapiens
0.026
-
5alpha-dihydrotestosterone
-
Homo sapiens
0.048
-
androsterone
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
liver
-
Homo sapiens
-
placenta
-
Homo sapiens
-
prostate gland
-
Homo sapiens
-
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0035
-
reduction of 5alpha-dihydrotestosteerone, cofactor NADPH, pH 6.0
Homo sapiens
0.0063
-
oxidation of 3alpha-androstanediol, cofactor NADP+, pH 9.0
Homo sapiens
0.0253
-
reduction of 5alpha-androstanedione, cofactor NADPH, pH 6.0
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha-androstanediol + NAD(P)+
-
389547
Homo sapiens
androsterone + NAD(P)H
-
389547
Homo sapiens
r
5alpha-dihydrotestosterone + NAD(P)H
-
389547
Homo sapiens
androstanedione + NAD(P)+
-
389547
Homo sapiens
r
testosterone + NAD(P)+
-
389547
Homo sapiens
androstenedione + NAD(P)H + H+
-
389547
Homo sapiens
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000367
-
androsterone
-
Homo sapiens
0.0009
-
testosterone
-
Homo sapiens
0.003
-
5alpha-dihydrotestosterone
-
Homo sapiens
0.00617
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
Homo sapiens
Other publictions for EC 1.1.1.209
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710716
Dhagat
Studies on a Tyr residue criti ...
Mus musculus
Acta Crystallogr. Sect. D
66
198-204
2010
-
-
-
1
3
-
-
36
-
-
-
-
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
38
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
36
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
38
-
-
-
-
-
-
-
-
36
36
695325
Dhagat
Structure of the G225P/G226P m ...
Mus musculus
Acta Crystallogr. Sect. D
65
257-265
2009
-
-
1
1
1
-
1
16
-
-
-
-
-
2
-
-
1
-
-
-
-
-
10
-
-
-
-
16
-
-
-
1
-
-
2
-
-
1
1
1
1
-
2
1
-
16
-
-
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
685538
Dhagat
Inhibition of 3(17)alpha-hydro ...
Mus musculus
Bioorg. Med. Chem.
16
3245-3254
2008
-
-
-
-
-
-
10
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
9
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684185
Dhagat
Structure of 3(17)alpha-hydrox ...
Mus musculus
Acta Crystallogr. Sect. F
63
825-830
2007
-
-
-
1
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667072
El-Kabbani
Crystallization and preliminar ...
Mus musculus
Acta crystallogr. Sect. F
61
688-690
2005
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389547
Lin
Expression and characterizatio ...
Homo sapiens
Mol. Endocrinol.
11
1971-1984
1997
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
5
3
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
5
3
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
286234
Nakagawa
Identification of two dihydrod ...
Mus musculus
J. Biochem.
106
633-638
1989
-
-
-
-
-
-
10
13
-
-
2
-
-
3
-
-
1
-
-
1
1
-
27
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
13
-
-
2
-
-
-
-
1
-
1
1
-
27
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286232
Lau
A 3(17) alpha-hydroxysteroid d ...
Oryctolagus cuniculus
J. Biol. Chem.
257
9444-9449
1982
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
1
5
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
-
1
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286233
Lau
Comparison of the multiple for ...
Oryctolagus cuniculus
J. Biol. Chem.
257
9450-9456
1982
-
-
-
-
-
-
1
6
1
-
-
-
-
1
-
-
-
-
-
2
-
-
6
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
6
1
-
-
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-