BRENDA - Enzyme Database show
show all sequences of 1.1.1.209

Comparison of the multiple forms of the soluble 3(17)alpha-hydroxysteroid dehydrogenases of female rabbit kidney and liver

Lau, P.C.K.; Layne, D.S.; Williamson, D.G.; J. Biol. Chem. 257, 9450-9456 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
androsterone
competitive vs. epitestosterone
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00053
-
Epitestosterone
liver isozyme IB
Oryctolagus cuniculus
0.00061
-
Epitestosterone
kidney isozyme II
Oryctolagus cuniculus
0.0012
-
androsterone
liver isozyme IC
Oryctolagus cuniculus
0.0021
0.0024
Epitestosterone
liver isozyme IB, androsterone, kidney, isozyme IB
Oryctolagus cuniculus
0.00291
-
Epitestosterone
kidney isozyme IB
Oryctolagus cuniculus
0.004
0.0047
androsterone
kidney isozyme II, liver isozyme IB
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
emale
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone + NAD(P)H
-
286233
Oryctolagus cuniculus
r
17alpha-estradiol 3-glucuronide + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone 3-glucuronide + NAD(P)H
-
286233
Oryctolagus cuniculus
r
3alpha-hydroxy-5alpha-androstan-17-one + NAD(P)+
-
286233
Oryctolagus cuniculus
5alpha-androstan-3,17-dione + NAD(P)H
-
286233
Oryctolagus cuniculus
?
5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
286233
Oryctolagus cuniculus
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H
i.e. 5alpha-dihydrotestosterone
286233
Oryctolagus cuniculus
r
epitestosterone + NAD(P)+
-
286233
Oryctolagus cuniculus
androst-4-en-3,17-dione + NAD(P)H + H+
-
286233
Oryctolagus cuniculus
r
estrone 3-sulfate + NAD(P)H
-
286233
Oryctolagus cuniculus
17alpha-estradiol 3-sulfate + NAD(P)+
-
286233
Oryctolagus cuniculus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
-
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Oryctolagus cuniculus
NADH
low activity
Oryctolagus cuniculus
NADP+
preferred
Oryctolagus cuniculus
NADPH
preferred
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Oryctolagus cuniculus
NADH
low activity
Oryctolagus cuniculus
NADP+
preferred
Oryctolagus cuniculus
NADPH
preferred
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
androsterone
competitive vs. epitestosterone
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00053
-
Epitestosterone
liver isozyme IB
Oryctolagus cuniculus
0.00061
-
Epitestosterone
kidney isozyme II
Oryctolagus cuniculus
0.0012
-
androsterone
liver isozyme IC
Oryctolagus cuniculus
0.0021
0.0024
Epitestosterone
liver isozyme IB, androsterone, kidney, isozyme IB
Oryctolagus cuniculus
0.00291
-
Epitestosterone
kidney isozyme IB
Oryctolagus cuniculus
0.004
0.0047
androsterone
kidney isozyme II, liver isozyme IB
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone + NAD(P)H
-
286233
Oryctolagus cuniculus
r
17alpha-estradiol 3-glucuronide + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone 3-glucuronide + NAD(P)H
-
286233
Oryctolagus cuniculus
r
3alpha-hydroxy-5alpha-androstan-17-one + NAD(P)+
-
286233
Oryctolagus cuniculus
5alpha-androstan-3,17-dione + NAD(P)H
-
286233
Oryctolagus cuniculus
?
5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
286233
Oryctolagus cuniculus
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H
i.e. 5alpha-dihydrotestosterone
286233
Oryctolagus cuniculus
r
epitestosterone + NAD(P)+
-
286233
Oryctolagus cuniculus
androst-4-en-3,17-dione + NAD(P)H + H+
-
286233
Oryctolagus cuniculus
r
estrone 3-sulfate + NAD(P)H
-
286233
Oryctolagus cuniculus
17alpha-estradiol 3-sulfate + NAD(P)+
-
286233
Oryctolagus cuniculus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
-
Oryctolagus cuniculus
Other publictions for EC 1.1.1.209
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710716
Dhagat
Studies on a Tyr residue criti ...
Mus musculus
Acta Crystallogr. Sect. D
66
198-204
2010
-
-
-
1
3
-
-
36
-
-
-
-
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
38
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
36
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
38
-
-
-
-
-
-
-
-
36
36
695325
Dhagat
Structure of the G225P/G226P m ...
Mus musculus
Acta Crystallogr. Sect. D
65
257-265
2009
-
-
1
1
1
-
1
16
-
-
-
-
-
2
-
-
1
-
-
-
-
-
10
-
-
-
-
16
-
-
-
1
-
-
2
-
-
1
1
1
1
-
2
1
-
16
-
-
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
685538
Dhagat
Inhibition of 3(17)alpha-hydro ...
Mus musculus
Bioorg. Med. Chem.
16
3245-3254
2008
-
-
-
-
-
-
10
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
9
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684185
Dhagat
Structure of 3(17)alpha-hydrox ...
Mus musculus
Acta Crystallogr. Sect. F
63
825-830
2007
-
-
-
1
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667072
El-Kabbani
Crystallization and preliminar ...
Mus musculus
Acta crystallogr. Sect. F
61
688-690
2005
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389547
Lin
Expression and characterizatio ...
Homo sapiens
Mol. Endocrinol.
11
1971-1984
1997
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
5
3
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
5
3
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
286234
Nakagawa
Identification of two dihydrod ...
Mus musculus
J. Biochem.
106
633-638
1989
-
-
-
-
-
-
10
13
-
-
2
-
-
3
-
-
1
-
-
1
1
-
27
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
13
-
-
2
-
-
-
-
1
-
1
1
-
27
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286232
Lau
A 3(17) alpha-hydroxysteroid d ...
Oryctolagus cuniculus
J. Biol. Chem.
257
9444-9449
1982
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
1
5
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
-
1
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286233
Lau
Comparison of the multiple for ...
Oryctolagus cuniculus
J. Biol. Chem.
257
9450-9456
1982
-
-
-
-
-
-
1
6
1
-
-
-
-
1
-
-
-
-
-
2
-
-
6
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
6
1
-
-
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-