BRENDA - Enzyme Database show
show all sequences of 1.1.1.208

Molecular cloning and characterization of a broad substrate terpenoid oxidoreductase from Artemisia annua

Ryden, A.; Ruyter-Spira, C.; Litjens, R.; Takahashi, S.; Quax, W.; Osada, H.; Bouwmeester, H.; Kayser, O.; Plant Cell Physiol. 51, 1219-1228 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Artemisia annua
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0071
-
(-)-menthone
pH 7.0, 30°C
Artemisia annua
0.305
-
(+)-neomenthol
pH 7.0, 30°C
Artemisia annua
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia annua
D1MI43
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-neomenthol + NADP+
reaction proceeds via (-)-menthone
726141
Artemisia annua
(-)-menthone + NADPH + H+
-
-
-
r
(-)-menthone + NADPH + H+
-
726141
Artemisia annua
(+)-neomenthol + NADP+
main product, plus some (-)-menthol
-
-
r
additional information
no substrates for reduction: carvone and isopiperitenone. No substrates in dehydrogenase reaction: (+)-borneol, (-)-borneol, (+)-menthol and (-)-neomenthol
726141
Artemisia annua
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.6
-
(-)-menthone
pH 7.0, 30°C
Artemisia annua
0.91
-
(+)-neomenthol
pH 7.0, 30°C
Artemisia annua
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Artemisia annua
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Artemisia annua
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Artemisia annua
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0071
-
(-)-menthone
pH 7.0, 30°C
Artemisia annua
0.305
-
(+)-neomenthol
pH 7.0, 30°C
Artemisia annua
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-neomenthol + NADP+
reaction proceeds via (-)-menthone
726141
Artemisia annua
(-)-menthone + NADPH + H+
-
-
-
r
(-)-menthone + NADPH + H+
-
726141
Artemisia annua
(+)-neomenthol + NADP+
main product, plus some (-)-menthol
-
-
r
additional information
no substrates for reduction: carvone and isopiperitenone. No substrates in dehydrogenase reaction: (+)-borneol, (-)-borneol, (+)-menthol and (-)-neomenthol
726141
Artemisia annua
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.6
-
(-)-menthone
pH 7.0, 30°C
Artemisia annua
0.91
-
(+)-neomenthol
pH 7.0, 30°C
Artemisia annua
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.99
-
(+)-neomenthol
pH 7.0, 30°C
Artemisia annua
83.6
-
(-)-menthone
pH 7.0, 30°C
Artemisia annua
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.99
-
(+)-neomenthol
pH 7.0, 30°C
Artemisia annua
83.6
-
(-)-menthone
pH 7.0, 30°C
Artemisia annua
Other publictions for EC 1.1.1.208
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726141
Ryden
Molecular cloning and characte ...
Artemisia annua
Plant Cell Physiol.
51
1219-1228
2010
-
-
1
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2
-
-
-
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-
1
-
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-
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3
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2
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1
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1
1
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2
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3
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2
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2
2
689629
Choi
A role for a menthone reductas ...
Arabidopsis thaliana, Capsicum annuum
Plant Physiol.
148
383-401
2008
1
-
2
-
2
-
-
-
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2
4
-
2
-
-
2
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4
-
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5
1
-
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-
3
-
-
2
-
1
-
1
-
2
2
-
2
-
-
-
-
-
-
-
2
4
-
-
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2
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4
-
-
5
1
-
-
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-
3
-
-
1
-
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-
-
-
670590
Davis
Monoterpene metabolism. Clonin ...
Mentha x piperita
Plant Physiol.
137
873-881
2005
-
-
1
-
-
-
-
4
-
-
1
3
-
3
-
-
1
-
-
2
-
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5
1
1
-
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2
1
1
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2
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1
2
-
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-
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4
-
-
1
3
-
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-
1
-
2
-
-
5
1
1
-
-
2
1
1
-
-
-
-
-
-
-
-
347974
Kjonaas
Metabolism of monoterpenes: Co ...
Mentha x piperita
Plant Physiol.
69
1013-1017
1982
-
-
-
-
-
-
4
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
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4
-
1
-
-
1
1
-
-
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1
-
-
-
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3
-
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1
-
-
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347975
Croteau
Demonstration of the intercell ...
Mentha x piperita
Plant Physiol.
69
975-977
1982
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-
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-
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1
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1
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1
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2
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1
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1
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2
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