BRENDA - Enzyme Database show
show all sequences of 1.1.1.208

A role for a menthone reductase in resistance against microbial pathogens in plants

Choi, H.W.; Lee, B.G.; Kim, N.H.; Park, Y.; Lim, C.W.; Song, H.K.; Hwang, B.K.; Plant Physiol. 148, 383-401 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
CaMNR1 is induced in leaves by bacterial infection and abiotic elicitor treatments. Under drought conditions, the CaMNR1 transcript is rapidly induced within 1 h and thereafter gradually increased to 25 h, while H2O2, cold and mechanical wounding treatment do not induce CaMNR1 expression in pepper leaves
Capsicum annuum
Cloned(Commentary)
Commentary
Organism
gene AtSDR1, overexpression in Escherichia coli
Arabidopsis thaliana
gene CaMNR1, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression in Escherichia coli and in transgenic Arabidopsis thaliana plants
Capsicum annuum
Engineering
Amino acid exchange
Commentary
Organism
additional information
AtSDR1 virus-induced gene silencing and ectopic overexpression in Arabidopsis thaliana plants leads to significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and lower expression levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, phenotype, overview
Arabidopsis thaliana
additional information
CaMNR1 tobacco rattle virus-mediated virus-induced gene silencing and ectopic overexpression in pepper and Arabidopsis thaliana plants, transgenic CaMNR1-silenced pepper plants are significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and express lower levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, while CaMNR1-overexpression transgenic Arabidopsis thaliana plants exhibit enhanced resistance to the hemi-biotrophic pathogen Pseudomonas syringae pv. tomato DC3000 and the biotrophic pathogen Hyaloperonospora parasitica isolate Noco2, accompanied by induction of AtPR1 and AtPDF1.2, phenotypes, overview
Capsicum annuum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Capsicum annuum
34700
-
1 * 34700, about, sequence calculation
Capsicum annuum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-menthone + NADPH
Arabidopsis thaliana
-
(+)-neomenthol + NADP+
-
-
r
(-)-menthone + NADPH
Capsicum annuum
in vitro antimicrobial activities of menthone, neomenthol and menthol, overview
(3S)-neomenthol + NADP+
-
-
r
additional information
Capsicum annuum
CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
?
-
-
-
additional information
Arabidopsis thaliana
the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9M2E2
pathogen responsive gene AtSDR1
-
Capsicum annuum
B2X050
pathogen responsive gene CaMNR1
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli to homogeneity
Arabidopsis thaliana
recombinant enzyme from Escherichia coli to homogeneity
Capsicum annuum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Capsicum annuum
-
fruit
in red, not in green, fruits
Capsicum annuum
-
leaf
the enzyme is induced in pepper leaves during compatible and incompatible interactions with Xcv, and by bacterial infection and abiotic elicitor treatments
Capsicum annuum
-
additional information
CaMNR1 is expressed in an organ-specific manner, no activity in uninduced leaves, stems, roots, or green fruits
Capsicum annuum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-menthone + NADPH
-
689629
Arabidopsis thaliana
(+)-neomenthol + NADP+
-
-
-
r
(-)-menthone + NADPH
in vitro antimicrobial activities of menthone, neomenthol and menthol, overview
689629
Capsicum annuum
(3S)-neomenthol + NADP+
-
-
-
r
(-)-menthone + NADPH
CaMNR1 converts (-)-menthone to 93% (3S)-neomenthol and 7% (3R)-menthol at pH 7.5, and to 72% (3S)-neomenthol and 28% (3R)-menthol at pH 9.0 with NADPH as a cofactor
689629
Capsicum annuum
(3S)-neomenthol + NADP+
-
-
-
r
additional information
CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
689629
Capsicum annuum
?
-
-
-
-
additional information
the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
689629
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 34700, about, sequence calculation
Capsicum annuum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
the pH alters the stereospecificity of the enzyme
Capsicum annuum
7.5
-
reduction reaction
Capsicum annuum
9
-
oxidation reaction
Capsicum annuum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Capsicum annuum
NADPH
the enzyme contains the coenzyme binding domain motif I: GxxxGxG
Capsicum annuum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Capsicum annuum
sequence calculation
-
5.39
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
CaMNR1 is induced in leaves by bacterial infection and abiotic elicitor treatments. Under drought conditions, the CaMNR1 transcript is rapidly induced within 1 h and thereafter gradually increased to 25 h, while H2O2, cold and mechanical wounding treatment do not induce CaMNR1 expression in pepper leaves
Capsicum annuum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene AtSDR1, overexpression in Escherichia coli
Arabidopsis thaliana
gene CaMNR1, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression in Escherichia coli and in transgenic Arabidopsis thaliana plants
Capsicum annuum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Capsicum annuum
NADPH
the enzyme contains the coenzyme binding domain motif I: GxxxGxG
Capsicum annuum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
AtSDR1 virus-induced gene silencing and ectopic overexpression in Arabidopsis thaliana plants leads to significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and lower expression levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, phenotype, overview
Arabidopsis thaliana
additional information
CaMNR1 tobacco rattle virus-mediated virus-induced gene silencing and ectopic overexpression in pepper and Arabidopsis thaliana plants, transgenic CaMNR1-silenced pepper plants are significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and express lower levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, while CaMNR1-overexpression transgenic Arabidopsis thaliana plants exhibit enhanced resistance to the hemi-biotrophic pathogen Pseudomonas syringae pv. tomato DC3000 and the biotrophic pathogen Hyaloperonospora parasitica isolate Noco2, accompanied by induction of AtPR1 and AtPDF1.2, phenotypes, overview
Capsicum annuum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Capsicum annuum
34700
-
1 * 34700, about, sequence calculation
Capsicum annuum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-menthone + NADPH
Arabidopsis thaliana
-
(+)-neomenthol + NADP+
-
-
r
(-)-menthone + NADPH
Capsicum annuum
in vitro antimicrobial activities of menthone, neomenthol and menthol, overview
(3S)-neomenthol + NADP+
-
-
r
additional information
Capsicum annuum
CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
?
-
-
-
additional information
Arabidopsis thaliana
the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli to homogeneity
Arabidopsis thaliana
recombinant enzyme from Escherichia coli to homogeneity
Capsicum annuum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Capsicum annuum
-
fruit
in red, not in green, fruits
Capsicum annuum
-
leaf
the enzyme is induced in pepper leaves during compatible and incompatible interactions with Xcv, and by bacterial infection and abiotic elicitor treatments
Capsicum annuum
-
additional information
CaMNR1 is expressed in an organ-specific manner, no activity in uninduced leaves, stems, roots, or green fruits
Capsicum annuum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-menthone + NADPH
-
689629
Arabidopsis thaliana
(+)-neomenthol + NADP+
-
-
-
r
(-)-menthone + NADPH
in vitro antimicrobial activities of menthone, neomenthol and menthol, overview
689629
Capsicum annuum
(3S)-neomenthol + NADP+
-
-
-
r
(-)-menthone + NADPH
CaMNR1 converts (-)-menthone to 93% (3S)-neomenthol and 7% (3R)-menthol at pH 7.5, and to 72% (3S)-neomenthol and 28% (3R)-menthol at pH 9.0 with NADPH as a cofactor
689629
Capsicum annuum
(3S)-neomenthol + NADP+
-
-
-
r
additional information
CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
689629
Capsicum annuum
?
-
-
-
-
additional information
the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview
689629
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 34700, about, sequence calculation
Capsicum annuum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
the pH alters the stereospecificity of the enzyme
Capsicum annuum
7.5
-
reduction reaction
Capsicum annuum
9
-
oxidation reaction
Capsicum annuum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Capsicum annuum
sequence calculation
-
5.39
Other publictions for EC 1.1.1.208
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726141
Ryden
Molecular cloning and characte ...
Artemisia annua
Plant Cell Physiol.
51
1219-1228
2010
-
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1
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2
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1
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2
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1
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1
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3
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2
2
689629
Choi
A role for a menthone reductas ...
Arabidopsis thaliana, Capsicum annuum
Plant Physiol.
148
383-401
2008
1
-
2
-
2
-
-
-
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2
4
-
2
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2
-
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4
-
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5
1
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3
-
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2
-
1
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1
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2
2
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2
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2
4
-
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2
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4
-
-
5
1
-
-
-
-
3
-
-
1
-
-
-
-
-
-
670590
Davis
Monoterpene metabolism. Clonin ...
Mentha x piperita
Plant Physiol.
137
873-881
2005
-
-
1
-
-
-
-
4
-
-
1
3
-
3
-
-
1
-
-
2
-
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5
1
1
-
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2
1
1
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2
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1
2
-
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4
-
-
1
3
-
-
-
1
-
2
-
-
5
1
1
-
-
2
1
1
-
-
-
-
-
-
-
-
347974
Kjonaas
Metabolism of monoterpenes: Co ...
Mentha x piperita
Plant Physiol.
69
1013-1017
1982
-
-
-
-
-
-
4
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
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1
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1
-
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1
-
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4
-
1
-
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1
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
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347975
Croteau
Demonstration of the intercell ...
Mentha x piperita
Plant Physiol.
69
975-977
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
1
-
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2
-
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1
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1
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2
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