BRENDA - Enzyme Database show
show all sequences of 1.1.1.208

Monoterpene metabolism. Cloning, expression, and characterization of menthone reductases from peppermint

Davis, E.M.; Ringer, K.L.; McConkey, M.E.; Croteau, R.; Plant Physiol. 137, 873-881 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
Mentha x piperita
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0054
-
NADP+
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
0.01
-
NADPH
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
0.674
-
(-)-menthone
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
1
-
(+)-isomenthone
above, pH 9.3, 31C, recombinant enzyme
Mentha x piperita
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35722
-
x * 35722, amino acid sequence calculation
Mentha x piperita
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-isomenthone + NADPH
Mentha x piperita
low activity
(+)-isomenthol + NADP+
-
-
?
(-)-menthone + NADPH
Mentha x piperita
-
(3S)-neomenthol + NADP+
-
-
r
additional information
Mentha x piperita
primary monoterpene biosynthesis pathway, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mentha x piperita
-
cv. Black Mitchum
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme partially
Mentha x piperita
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Mentha x piperita
-
oil gland secretory cell
-
Mentha x piperita
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-isomenthone + NADPH
low activity
670590
Mentha x piperita
(+)-isomenthol + NADP+
-
-
-
?
(-)-menthone + NADPH
-
670590
Mentha x piperita
(3S)-neomenthol + NADP+
-
-
-
r
(-)-menthone + NADPH
low activity in the reverse reaction
670590
Mentha x piperita
(3S)-neomenthol + NADP+
(3S)-neomenthol is the main product, product determination by gas chromatography, overview
-
-
r
additional information
primary monoterpene biosynthesis pathway, overview
670590
Mentha x piperita
?
-
-
-
-
additional information
monoterpenol is a poor substrate
670590
Mentha x piperita
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 35722, amino acid sequence calculation
Mentha x piperita
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
31
-
assay at
Mentha x piperita
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
(-)-menthone
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
0.06
-
NADPH
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Mentha x piperita
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
10.5
50% of maximal activity at pH 6.5 and pH 10.5
Mentha x piperita
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Mentha x piperita
NADPH
-
Mentha x piperita
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
Mentha x piperita
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Mentha x piperita
NADPH
-
Mentha x piperita
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0054
-
NADP+
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
0.01
-
NADPH
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
0.674
-
(-)-menthone
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
1
-
(+)-isomenthone
above, pH 9.3, 31C, recombinant enzyme
Mentha x piperita
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35722
-
x * 35722, amino acid sequence calculation
Mentha x piperita
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-isomenthone + NADPH
Mentha x piperita
low activity
(+)-isomenthol + NADP+
-
-
?
(-)-menthone + NADPH
Mentha x piperita
-
(3S)-neomenthol + NADP+
-
-
r
additional information
Mentha x piperita
primary monoterpene biosynthesis pathway, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme partially
Mentha x piperita
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Mentha x piperita
-
oil gland secretory cell
-
Mentha x piperita
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-isomenthone + NADPH
low activity
670590
Mentha x piperita
(+)-isomenthol + NADP+
-
-
-
?
(-)-menthone + NADPH
-
670590
Mentha x piperita
(3S)-neomenthol + NADP+
-
-
-
r
(-)-menthone + NADPH
low activity in the reverse reaction
670590
Mentha x piperita
(3S)-neomenthol + NADP+
(3S)-neomenthol is the main product, product determination by gas chromatography, overview
-
-
r
additional information
primary monoterpene biosynthesis pathway, overview
670590
Mentha x piperita
?
-
-
-
-
additional information
monoterpenol is a poor substrate
670590
Mentha x piperita
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 35722, amino acid sequence calculation
Mentha x piperita
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
31
-
assay at
Mentha x piperita
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
(-)-menthone
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
0.06
-
NADPH
pH 9.3, 31C, recombinant enzyme
Mentha x piperita
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Mentha x piperita
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
10.5
50% of maximal activity at pH 6.5 and pH 10.5
Mentha x piperita
Other publictions for EC 1.1.1.208
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726141
Ryden
Molecular cloning and characte ...
Artemisia annua
Plant Cell Physiol.
51
1219-1228
2010
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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2
-
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-
-
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2
2
689629
Choi
A role for a menthone reductas ...
Arabidopsis thaliana, Capsicum annuum
Plant Physiol.
148
383-401
2008
1
-
2
-
2
-
-
-
-
-
2
4
-
2
-
-
2
-
-
4
-
-
5
1
-
-
-
-
3
-
-
2
-
1
-
1
-
2
2
-
2
-
-
-
-
-
-
-
2
4
-
-
-
2
-
4
-
-
5
1
-
-
-
-
3
-
-
1
-
-
-
-
-
-
670590
Davis
Monoterpene metabolism. Clonin ...
Mentha x piperita
Plant Physiol.
137
873-881
2005
-
-
1
-
-
-
-
4
-
-
1
3
-
3
-
-
1
-
-
2
-
-
5
1
1
-
-
2
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
-
-
1
3
-
-
-
1
-
2
-
-
5
1
1
-
-
2
1
1
-
-
-
-
-
-
-
-
347974
Kjonaas
Metabolism of monoterpenes: Co ...
Mentha x piperita
Plant Physiol.
69
1013-1017
1982
-
-
-
-
-
-
4
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
1
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
347975
Croteau
Demonstration of the intercell ...
Mentha x piperita
Plant Physiol.
69
975-977
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
1
-
-
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-
1
-
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2
-
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