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show all sequences of 1.1.1.206

Molecular cloning and catalytic characterization of a recombinant tropine biosynthetic tropinone reductase from Withania coagulans leaf

Kushwaha, A.K.; Sangwan, N.S.; Tripathi, S.; Sangwan, R.S.; Gene 516, 238-247 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene TRI, isolated from aerial tissue, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3); gene WcTR-I, from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Withania coagulans
Inhibitors
Inhibitors
Commentary
Organism
Structure
K+
slight inhibitory effect at 5 mM
Withania coagulans
Li+
slight inhibitory effect at 5 mM
Withania coagulans
Na+
slight inhibitory effect at 5 mM
Withania coagulans
NH4+
slight inhibitory effect at 5 mM
Withania coagulans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
the in vitro reaction kinetics predominantly favour the reaction direction of tropinone reduction, Michaelis-Menten kinetics
Withania coagulans
0.126
-
NADPH
pH 6.6, 30C, recombinant enzyme
Withania coagulans
0.127
-
NADPH
pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
0.208
-
NADP+
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
1.451
-
tropinone
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
26.31
-
Tropine
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29340
-
x * 29340, sequence calculation
Withania coagulans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Withania coagulans
the enzyme TR-I has a wide substrate specificity but does not cover the substrates of other well-known plant SDR related to menthol metabolism
?
-
-
-
tropinone + NADPH + H+
Withania coagulans
preferred substrate
tropine + NADP+
-
-
r
tropinone + NADPH + H+
Withania coagulans
the reaction direction of tropinone reduction is predominantly operational in planta
tropine + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Withania coagulans
L7QI79
-
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography; recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
Withania coagulans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Withania coagulans
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-methylpyrrolidine + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
1-propyl-4-piperidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
2-pyrrolidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
3-methylcyclohexanone + NADP+
-
725098
Withania coagulans
3-methylcyclohexanol + NADP+
-
-
-
?
3-methylcyclohexanone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
4-chlor-1-methylpiperidine + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
4-ethylcyclohexanone + NADPH + H+
-
725098
Withania coagulans
4-ethylcyclohexanol + NADP+
-
-
-
?
4-methylcyclohexanone + NADPH + H+
-
725098
Withania coagulans
4-methylcyclohexanol + NADP+
-
-
-
?
4-piperidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
exo-6-hydroxytropinone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
additional information
the enzyme TR-I has a wide substrate specificity but does not cover the substrates of other well-known plant SDR related to menthol metabolism
725098
Withania coagulans
?
-
-
-
-
additional information
no activity by TR-I with 1-methyl-2-pyrrolidone
725098
Withania coagulans
?
-
-
-
-
additional information
wide substrate specificity, overview. No activity with 1-methyl-2-pyrrolidone. The reverse reaction of tropine oxidation is highly specific and none of the tested alcohols can be oxidized with either NADP+ or NAD+ as cofactor
725098
Withania coagulans
?
-
-
-
-
N-methyl-4-piperidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
N-methylpiperidine + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
tetrahydro-4H-thiopyran-4-one + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
tropine + NADP+
in vitro reaction kinetics show that the enzyme predominantly favours the reverse reaction
725098
Withania coagulans
tropinone + NADPH + H+
-
-
-
r
tropinone + NADPH + H+
best substrate
725098
Withania coagulans
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
preferred substrate
725098
Withania coagulans
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
the reaction direction of tropinone reduction is predominantly operational in planta
725098
Withania coagulans
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
the in vitro reaction kinetics predominantly favour the reaction direction of tropinone reduction
725098
Withania coagulans
tropine + NADPH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 29340, sequence calculation
Withania coagulans
More
a three dimensional model is prepared by taking Datura stramonium TR-II, PDB ID 1ipf, as template, structure comparison, overview; superimposition of three-dimensional models of tropinone reductases,overview
Withania coagulans
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Withania coagulans
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
80
purified recombinant His-tagged enzyme, pH 6.6, 30 min, over 92% activity remaining at up to 50C, 30% remaining at 60C, 7% at 70C, complete inactivation occurs at 80C
Withania coagulans
50
-
purified recombinant enzyme, 30 min, over 92% of maximal activity remaining
Withania coagulans
60
-
purified recombinant enzyme, 30 min, 30% of maximal activity remaining
Withania coagulans
70
-
purified recombinant enzyme, 30 min, 7% of maximal activity remaining
Withania coagulans
80
-
purified recombinant enzyme, 30 min, inactivation
Withania coagulans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.87
-
Tropine
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
2.94
-
NADP+
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
6.76
-
NADPH
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
16.74
-
tropinone
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
; tropinone reduction
Withania coagulans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
narrow pH range in tropinone reduction
Withania coagulans
6.1
7
narrow pH range
Withania coagulans
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme has absolute specificity for NADPH/NADP+. The affinity of the enzyme for NADPH is much higher than for NADP+ compared to NADPH
Withania coagulans
NADP+
-
Withania coagulans
NADPH
-
Withania coagulans
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Withania coagulans
sequence calculation
-
6.12
Cloned(Commentary) (protein specific)
Commentary
Organism
gene TRI, isolated from aerial tissue, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3); gene WcTR-I, from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Withania coagulans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme has absolute specificity for NADPH/NADP+. The affinity of the enzyme for NADPH is much higher than for NADP+ compared to NADPH
Withania coagulans
NADP+
-
Withania coagulans
NADPH
-
Withania coagulans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
K+
slight inhibitory effect at 5 mM
Withania coagulans
Li+
slight inhibitory effect at 5 mM
Withania coagulans
Na+
slight inhibitory effect at 5 mM
Withania coagulans
NH4+
slight inhibitory effect at 5 mM
Withania coagulans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
the in vitro reaction kinetics predominantly favour the reaction direction of tropinone reduction, Michaelis-Menten kinetics
Withania coagulans
0.126
-
NADPH
pH 6.6, 30C, recombinant enzyme
Withania coagulans
0.127
-
NADPH
pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
0.208
-
NADP+
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
1.451
-
tropinone
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
26.31
-
Tropine
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29340
-
x * 29340, sequence calculation
Withania coagulans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Withania coagulans
the enzyme TR-I has a wide substrate specificity but does not cover the substrates of other well-known plant SDR related to menthol metabolism
?
-
-
-
tropinone + NADPH + H+
Withania coagulans
preferred substrate
tropine + NADP+
-
-
r
tropinone + NADPH + H+
Withania coagulans
the reaction direction of tropinone reduction is predominantly operational in planta
tropine + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography; recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
Withania coagulans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Withania coagulans
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-methylpyrrolidine + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
1-propyl-4-piperidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
2-pyrrolidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
3-methylcyclohexanone + NADP+
-
725098
Withania coagulans
3-methylcyclohexanol + NADP+
-
-
-
?
3-methylcyclohexanone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
4-chlor-1-methylpiperidine + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
4-ethylcyclohexanone + NADPH + H+
-
725098
Withania coagulans
4-ethylcyclohexanol + NADP+
-
-
-
?
4-methylcyclohexanone + NADPH + H+
-
725098
Withania coagulans
4-methylcyclohexanol + NADP+
-
-
-
?
4-piperidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
exo-6-hydroxytropinone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
additional information
the enzyme TR-I has a wide substrate specificity but does not cover the substrates of other well-known plant SDR related to menthol metabolism
725098
Withania coagulans
?
-
-
-
-
additional information
no activity by TR-I with 1-methyl-2-pyrrolidone
725098
Withania coagulans
?
-
-
-
-
additional information
wide substrate specificity, overview. No activity with 1-methyl-2-pyrrolidone. The reverse reaction of tropine oxidation is highly specific and none of the tested alcohols can be oxidized with either NADP+ or NAD+ as cofactor
725098
Withania coagulans
?
-
-
-
-
N-methyl-4-piperidone + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
N-methylpiperidine + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
tetrahydro-4H-thiopyran-4-one + NADPH + H+
-
725098
Withania coagulans
? + NADP+
-
-
-
?
tropine + NADP+
in vitro reaction kinetics show that the enzyme predominantly favours the reverse reaction
725098
Withania coagulans
tropinone + NADPH + H+
-
-
-
r
tropinone + NADPH + H+
best substrate
725098
Withania coagulans
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
preferred substrate
725098
Withania coagulans
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
the reaction direction of tropinone reduction is predominantly operational in planta
725098
Withania coagulans
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
the in vitro reaction kinetics predominantly favour the reaction direction of tropinone reduction
725098
Withania coagulans
tropine + NADPH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 29340, sequence calculation
Withania coagulans
More
a three dimensional model is prepared by taking Datura stramonium TR-II, PDB ID 1ipf, as template, structure comparison, overview; superimposition of three-dimensional models of tropinone reductases,overview
Withania coagulans
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Withania coagulans
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
80
purified recombinant His-tagged enzyme, pH 6.6, 30 min, over 92% activity remaining at up to 50C, 30% remaining at 60C, 7% at 70C, complete inactivation occurs at 80C
Withania coagulans
50
-
purified recombinant enzyme, 30 min, over 92% of maximal activity remaining
Withania coagulans
60
-
purified recombinant enzyme, 30 min, 30% of maximal activity remaining
Withania coagulans
70
-
purified recombinant enzyme, 30 min, 7% of maximal activity remaining
Withania coagulans
80
-
purified recombinant enzyme, 30 min, inactivation
Withania coagulans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.87
-
Tropine
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
2.94
-
NADP+
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
6.76
-
NADPH
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
16.74
-
tropinone
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
; tropinone reduction
Withania coagulans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
narrow pH range in tropinone reduction
Withania coagulans
6.1
7
narrow pH range
Withania coagulans
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Withania coagulans
sequence calculation
-
6.12
General Information
General Information
Commentary
Organism
evolution
tropinone reductases (TRs) are small proteins belonging to the SDR (short chain dehydrogenase/reductase) family of enzymes. The enzyme's sequence contains the signature YXXXK motif of SDRs. Residues His112, Ala160, Val168, Ile223 and Phe226 are conserved in the TRs, and conserved at corresponding positions in WcTR-I, and involved in stereospecificity of the respective TR-Is. Tropane alkaloids might have evolved independently in plants, at least in Solanaceae and Erythroxylaceae; tropinone reductase TR-I belongs to the short chain dehydrogenase/reductase family, SDR, of enzymes and has a YSASK as the signature YXXXK motif of SDRs, catalytic tetrade comprises N, S, Y, and K residues, overview
Withania coagulans
metabolism
enzymes TR-I and TR-II catalyze the conversion of tropinone into tropane alcohols (tropine and pseudotropine, respectively). The steps are intermediary enroute to biosynthesis of tropane esters of medicinal importance, hyoscyamine/scopolamine, and calystegins, respectively. The enzyme has a wide substrate specificity but does not cover the substrates of other plant SDR enzymes related to menthol metabolism. The leaf tissue expression of the enzyme with these catalytic features suggests physiologically much prodigal rate of operation of the tropane alkaloid pathway in the leaf tissue itself; the enzyme catalyzes a step intermediary enroute to biosynthesis of tropane esters of medicinal importance, i.e. hyoscyamine/scopolamine
Withania coagulans
additional information
a three dimensional model is prepared by taking Datura stramonium TR-II, PDB ID 1ipf, as template, structure comparison, overview; superimposition of three-dimensional models of tropinone reductases,overview
Withania coagulans
General Information (protein specific)
General Information
Commentary
Organism
evolution
tropinone reductases (TRs) are small proteins belonging to the SDR (short chain dehydrogenase/reductase) family of enzymes. The enzyme's sequence contains the signature YXXXK motif of SDRs. Residues His112, Ala160, Val168, Ile223 and Phe226 are conserved in the TRs, and conserved at corresponding positions in WcTR-I, and involved in stereospecificity of the respective TR-Is. Tropane alkaloids might have evolved independently in plants, at least in Solanaceae and Erythroxylaceae; tropinone reductase TR-I belongs to the short chain dehydrogenase/reductase family, SDR, of enzymes and has a YSASK as the signature YXXXK motif of SDRs, catalytic tetrade comprises N, S, Y, and K residues, overview
Withania coagulans
metabolism
enzymes TR-I and TR-II catalyze the conversion of tropinone into tropane alcohols (tropine and pseudotropine, respectively). The steps are intermediary enroute to biosynthesis of tropane esters of medicinal importance, hyoscyamine/scopolamine, and calystegins, respectively. The enzyme has a wide substrate specificity but does not cover the substrates of other plant SDR enzymes related to menthol metabolism. The leaf tissue expression of the enzyme with these catalytic features suggests physiologically much prodigal rate of operation of the tropane alkaloid pathway in the leaf tissue itself; the enzyme catalyzes a step intermediary enroute to biosynthesis of tropane esters of medicinal importance, i.e. hyoscyamine/scopolamine
Withania coagulans
additional information
a three dimensional model is prepared by taking Datura stramonium TR-II, PDB ID 1ipf, as template, structure comparison, overview; superimposition of three-dimensional models of tropinone reductases,overview
Withania coagulans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.071
-
Tropine
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
11.53
-
tropinone
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
14
-
NADP+
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
53
-
NADPH
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.071
-
Tropine
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
11.53
-
tropinone
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
14
-
NADP+
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
53
-
NADPH
pH 6.6, 30C, recombinant enzyme; pH 6.6, 30C, recombinant His-tagged enzyme
Withania coagulans
Other publictions for EC 1.1.1.206
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741221
Kuester
Potato plants with genetically ...
Solanum tuberosum
Planta
245
355-365
2017
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1
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1
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2
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2
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3
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1
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3
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7
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7
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7
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7
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27
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4
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2
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2
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2
2
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2
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2
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1
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1
1
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4
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5
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Specificities of the enzymes o ...
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Nakajima
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1
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1
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1
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1
1
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2
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3
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2
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1
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1
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1
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1
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Two tropinone reductases with ...
Atropa acuminata, Atropa belladonna, Datura stramonium, Duboisia hopwoodii, Duboisia leichhardtii, Hyoscyamus albus, Hyoscyamus bohemicus, Hyoscyamus canariensis, Hyoscyamus muticus, Hyoscyamus niger, Hyoscyamus niger Hn, Hyoscyamus pusillus, no activity in Brassica campestris, no activity in Browallia americana, no activity in Calystegia sepium, no activity in Nicotiana tabacum, no activity in Physalis alkekengi, Physalis edulis, Physalis philadelphica, Physochlaina orientalis
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16
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11
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4
15
1
18
1
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4
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1
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4
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2
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1
2
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-
-
-
-
-
2
-
-
-
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1
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7
-
-
1
1
-
-
-
1
-
1
-
4
4
-
1
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4
-
2
-
1
-
-
-
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-