BRENDA - Enzyme Database show
show all sequences of 1.1.1.206

Tropine dehydrogenase: purification, some properties and an evaluation of its role in the bacterial metabolism of tropine

Bartholomew, B.A.; Smith, M.J.; Long, M.T.; Darcy, P.J.; Trudgill P.W.; Hopper, K.J.; Biochem. J. 307, 603-608 (1995)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
Tropine
-
Pseudomonas sp.
0.048
-
NADP+
-
Pseudomonas sp.
0.073
-
nortropine
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
SDS-PAGE
Pseudomonas sp.
58000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropine + NADP+
Pseudomonas sp.
role in the pathway for tropine catabolism
tropinone + NADPH
-
-
r
tropine + NADP+
Pseudomonas sp. AT3
role in the pathway for tropine catabolism
tropinone + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
AT3
-
Pseudomonas sp. AT3
-
AT3
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.316
-
crude extract
Pseudomonas sp.
63.82
-
purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nortropine + NADP+
-
347970
Pseudomonas sp.
nortropinone + NADPH
-
-
-
?
nortropine + NADP+
-
347970
Pseudomonas sp. AT3
nortropinone + NADPH
-
-
-
?
tropine + NADP+
-
347970
Pseudomonas sp.
tropinone + NADPH
-
347970
Pseudomonas sp.
r
tropine + NADP+
role in the pathway for tropine catabolism
347970
Pseudomonas sp.
tropinone + NADPH
-
-
-
r
tropine + NADP+
-
347970
Pseudomonas sp. AT3
tropinone + NADPH
-
347970
Pseudomonas sp. AT3
r
tropine + NADP+
role in the pathway for tropine catabolism
347970
Pseudomonas sp. AT3
tropinone + NADPH
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 identical subunits, gel filtration
Pseudomonas sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
loss of 65% activity after 60 hours in crude extracts; loss of 90% activity of the purified enzyme after 10 hours, presence of 10% ethanol during the purification procedure
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+
Pseudomonas sp.
NADP+
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+
Pseudomonas sp.
NADP+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
Tropine
-
Pseudomonas sp.
0.048
-
NADP+
-
Pseudomonas sp.
0.073
-
nortropine
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
SDS-PAGE
Pseudomonas sp.
58000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropine + NADP+
Pseudomonas sp.
role in the pathway for tropine catabolism
tropinone + NADPH
-
-
r
tropine + NADP+
Pseudomonas sp. AT3
role in the pathway for tropine catabolism
tropinone + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.316
-
crude extract
Pseudomonas sp.
63.82
-
purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nortropine + NADP+
-
347970
Pseudomonas sp.
nortropinone + NADPH
-
-
-
?
nortropine + NADP+
-
347970
Pseudomonas sp. AT3
nortropinone + NADPH
-
-
-
?
tropine + NADP+
-
347970
Pseudomonas sp.
tropinone + NADPH
-
347970
Pseudomonas sp.
r
tropine + NADP+
role in the pathway for tropine catabolism
347970
Pseudomonas sp.
tropinone + NADPH
-
-
-
r
tropine + NADP+
-
347970
Pseudomonas sp. AT3
tropinone + NADPH
-
347970
Pseudomonas sp. AT3
r
tropine + NADP+
role in the pathway for tropine catabolism
347970
Pseudomonas sp. AT3
tropinone + NADPH
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 identical subunits, gel filtration
Pseudomonas sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
loss of 65% activity after 60 hours in crude extracts; loss of 90% activity of the purified enzyme after 10 hours, presence of 10% ethanol during the purification procedure
Pseudomonas sp.
Other publictions for EC 1.1.1.206
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725098
Kushwaha
Molecular cloning and catalyti ...
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516
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1
-
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4
4
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1
2
-
4
-
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1
-
-
2
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17
2
1
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4
4
1
1
-
2
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1
2
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4
-
4
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1
2
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1
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2
-
-
17
2
1
-
4
4
1
1
-
-
-
3
3
-
4
4
726209
Dehghan
An atypical pattern of accumul ...
Hyoscyamus muticus, Hyoscyamus senecionis
Plant Physiol. Biochem.
70C
188-194
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-
-
-
-
-
-
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2
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4
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3
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2
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-
2
2
-
-
-
725946
Kai
Enhancing the production of tr ...
Anisodus acutangulus
Mol. Biosyst.
8
2883-2890
2012
-
1
-
-
-
-
-
-
-
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3
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1
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1
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1
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719169
Kai
Co-expression of AaPMT and AaT ...
Anisodus acutangulus
BMC Biotechnol.
11
43
2011
-
1
-
-
-
-
-
-
-
-
-
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3
-
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1
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1
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1
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-
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-
-
-
-
696874
Kai
Molecular cloning, characteriz ...
Anisodus acutangulus
Biotechnol. Appl. Biochem.
54
:177-186
2009
1
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
689548
Brock
The functional divergence of s ...
Cochlearia officinalis
Plant J.
54
388-401
2008
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
1
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1
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2
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4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689953
Freydank
Protein structure modeling ind ...
Solanum dulcamara
Proteins
72
173-183
2008
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
2
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2
-
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1
2
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1
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1
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670518
Draeger
Tropinone reductases, enzymes ...
Atropa belladonna, Datura stramonium, Duboisia leichhardtii, Duboisia myoporoides, Hyoscyamus muticus, Hyoscyamus niger, Scopolia atropoides
Phytochemistry
67
327-337
2006
-
-
-
-
-
-
-
7
-
-
-
7
-
9
-
-
-
-
-
-
-
-
27
-
-
-
-
1
4
-
-
7
-
-
-
-
-
-
7
-
-
-
-
-
-
7
-
-
-
7
-
-
-
-
-
-
-
-
27
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
656413
Richter
Overexpression of tropinone re ...
Atropa belladonna, Datura stramonium
J. Exp. Bot.
56
645-652
2005
-
-
2
-
2
-
-
-
-
-
-
2
-
2
-
-
1
-
-
1
1
-
4
-
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
1
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
439278
Rocha
-
Functional expression of tropi ...
Hyoscyamus niger
Plant Sci.
162
905-913
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347972
Nakajima
Site-directed mutagenesis of p ...
Datura stramonium
J. Biol. Chem.
274
16563-16568
1999
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347973
Boswell
Specificities of the enzymes o ...
Brugmansia sp., Datura stramonium
Phytochemistry
52
871-878
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
2
-
-
2
-
-
25
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
25
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348028
Nakajima
Crystal structures of two trop ...
Datura stramonium
Proc. Natl. Acad. Sci. USA
95
4876-4881
1998
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347970
Bartholomew
-
Tropine dehydrogenase: purific ...
Pseudomonas sp., Pseudomonas sp. AT3
Biochem. J.
307
603-608
1995
-
-
-
-
-
-
-
3
-
-
2
2
-
4
-
-
1
-
-
-
2
-
6
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
2
2
-
-
-
1
-
-
2
-
6
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
347969
Nakajima
Opposite stereospecificity of ...
Datura stramonium
J. Biol. Chem.
269
11695-11698
1994
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
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-
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-
-
-
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-
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-
1
-
-
1
-
-
-
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-
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-
-
1
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1
-
2
-
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-
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-
-
-
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-
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-
-
-
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-
348024
Hashimoto
Two tropinone reductases with ...
Atropa acuminata, Atropa belladonna, Datura stramonium, Duboisia hopwoodii, Duboisia leichhardtii, Hyoscyamus albus, Hyoscyamus bohemicus, Hyoscyamus canariensis, Hyoscyamus muticus, Hyoscyamus niger, Hyoscyamus niger Hn, Hyoscyamus pusillus, no activity in Brassica campestris, no activity in Browallia americana, no activity in Calystegia sepium, no activity in Nicotiana tabacum, no activity in Physalis alkekengi, Physalis edulis, Physalis philadelphica, Physochlaina orientalis
Plant Physiol.
100
836-845
1992
-
-
-
-
-
-
16
11
-
-
2
-
-
20
-
-
1
-
-
4
15
1
18
1
-
-
-
-
2
-
-
3
-
-
-
-
-
-
3
-
-
-
-
16
-
11
-
-
2
-
-
-
-
1
-
4
15
1
18
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
347967
Koelen
Partial purification and prope ...
Datura stramonium
J. Med. Plant Res.
44
227-230
1982
-
-
-
-
-
-
1
7
-
-
1
1
-
2
-
-
1
-
-
1
-
4
4
-
1
-
4
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
1
-
7
-
-
1
1
-
-
-
1
-
1
-
4
4
-
1
-
4
-
2
-
1
-
-
-
-
-
-
-