Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cs+ | 36% of the activity with K+ | Bacillus anthracis | |
dimethyl sulfoxide | up to 15-20% increase IMP dehydrogenase activity | Bacillus anthracis | |
K+ | optimal activation at 100-150 mM K+ | Bacillus anthracis | |
Li+ | less than 1% of the activity with K+ | Bacillus anthracis | |
additional information | strict requirement for a monovalent cation | Bacillus anthracis | |
Na+ | less than 1% of the activity with K+ | Bacillus anthracis | |
NH4+ | 77% of the activity with K+ | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
in a phosphate ion-bound form and in complex with its substrate, inosine 5'-monophosphate, and product, xanthosine 5'-monophosphate, to 2.38-2.65 A resolution. The enzyme monomer has a typical two-domain structure, the catalytic domain, which is a TIM barrel, and the CBS domain. In all structures, each monomer contains a ligand bound in the active site, i.e.phosphate anion in the apo structure and IMP and XMP in the substrate and product-bound structures, respectively. In all the structures, the CBS domains are partially disordered | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide | - |
Bacillus anthracis | |
4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide | - |
Campylobacter jejuni | |
Mycophenolic acid | noncompetitive with regard to inosine monophosphate and NAD+ | Bacillus anthracis | |
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | - |
Bacillus anthracis | |
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | - |
Campylobacter jejuni | |
NAD+ | - |
Bacillus anthracis | |
xanthosine 5'-phosphate | competitive with regard to inosine monophosphate | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
Inosine 5'-diphosphate | pH 8.0, 22°C | Bacillus anthracis | |
0.55 | - |
NAD+ | pH 8.0, 22°C | Bacillus anthracis |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl sulfoxide | up to 15-20% increase IMP dehydrogenase activity | Campylobacter jejuni |
dimethyl sulfoxide | up to 15-20% increase IMP dehydrogenase activity | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81W29 | - |
- |
Campylobacter jejuni | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-diphosphate + NAD+ + H2O | - |
Campylobacter jejuni | xanthosine 5'-diphosphate + NADH + H+ | - |
? | |
inosine 5'-diphosphate + NAD+ + H2O | - |
Bacillus anthracis | xanthosine 5'-diphosphate + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
Inosine 5'-diphosphate | pH 8.0, 22°C | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Campylobacter jejuni | |
NAD+ | - |
Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
Mycophenolic acid | substrate NAD+, pH 8.0, 22°C | Bacillus anthracis | |
0.0048 | - |
Mycophenolic acid | substrate inosine monophosphate, pH 8.0, 22°C | Bacillus anthracis | |
0.218 | - |
xanthosine 5'-phosphate | pH 8.0, 22°C | Bacillus anthracis | |
3.9 | - |
NAD+ | pH 8.0, 22°C | Bacillus anthracis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000051 | - |
pH 8.0, 22°C | Campylobacter jejuni | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.000057 | - |
pH 8.0, 22°C | Bacillus anthracis | 4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide | |
0.000057 | - |
pH 8.0, 22°C | Bacillus anthracis | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.00012 | - |
pH 8.0, 22°C | Campylobacter jejuni | 4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide |