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Literature summary for 1.1.1.205 extracted from

  • Pugh, M.E.; Skibo, E.B.
    Inosine monophosphate dehydrogenase from porcine (Sus scrofa domestica) thymus: Purification and properties (1993), Comp. Biochem. Physiol. B, 2, 381-387.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-Chloro-9-beta-D-ribofuranosylpurine 5 '-phosphate acts at the IMP binding site Sus scrofa
AMP
-
Sus scrofa
GMP
-
Sus scrofa
NAD+ 44% inhibition at 1.5 M Sus scrofa
NH4+ 25% inhibition at 2.12 M Sus scrofa
xanthosine 5'-phosphate
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
IMP
-
Sus scrofa
0.016
-
NAD+
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.57
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-20, no significant loss of activity for serveral weeks after ammonium sulfate precipitation, no loss of activity after Sephacryl S400 chromatography Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IMP + NAD+ + H2O
-
Sus scrofa XMP + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: about 50% of activity maximum, pH 9: about 50% of activity maximum Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
GMP
-
Sus scrofa
0.085
-
xanthosine 5'-phosphate
-
Sus scrofa
0.282
-
AMP
-
Sus scrofa