Cloned (Comment) | Organism |
---|---|
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces viridochromogenes |
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas syringae pv. tomato |
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Fulvimarina pelagi |
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oceanicola granulosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Streptomyces viridochromogenes | |
additional information | - |
additional information | Michaelis-Menten kinetics | Pseudomonas syringae pv. tomato | |
additional information | - |
additional information | Michaelis-Menten kinetics | Agrobacterium tumefaciens | |
additional information | - |
additional information | Michaelis-Menten kinetics | Fulvimarina pelagi | |
additional information | - |
additional information | Michaelis-Menten kinetics | Oceanicola granulosus | |
0.02 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
0.03 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
0.03 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
0.04 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
0.1 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
0.1 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
0.1 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
0.14 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
0.2 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
0.2 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
0.2 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
0.2 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
0.2 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
0.3 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
0.3 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
0.3 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
0.4 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
0.5 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
1 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
6.4 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
7.3 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
8.3 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
9 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
9.5 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
13.6 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
19.4 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
22.05 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
55 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
59 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
64 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
64 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
112 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
142 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
465 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
628 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galacturonate + NAD+ | Streptomyces viridochromogenes | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Pseudomonas syringae pv. tomato | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Agrobacterium tumefaciens | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Fulvimarina pelagi | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Oceanicola granulosus | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Oceanicola granulosus DSM 15982 | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Streptomyces viridochromogenes DSM 40736 | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Pseudomonas syringae pv. tomato DC3000 | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Streptomyces viridochromogenes | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Pseudomonas syringae pv. tomato | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Agrobacterium tumefaciens | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Fulvimarina pelagi | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Oceanicola granulosus | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Oceanicola granulosus DSM 15982 | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Streptomyces viridochromogenes DSM 40736 | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Pseudomonas syringae pv. tomato DC3000 | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | Q7CRQ0 | - |
- |
Agrobacterium tumefaciens C58 / ATCC 33970 | Q7CRQ0 | - |
- |
Fulvimarina pelagi | - |
- |
- |
Oceanicola granulosus | - |
- |
- |
Oceanicola granulosus DSM 15982 | - |
- |
- |
Pseudomonas syringae pv. tomato | Q888H1 | - |
- |
Pseudomonas syringae pv. tomato DC3000 | Q888H1 | - |
- |
Streptomyces viridochromogenes | - |
- |
- |
Streptomyces viridochromogenes DSM 40736 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galacturonate + NAD+ | - |
Streptomyces viridochromogenes | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Pseudomonas syringae pv. tomato | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Agrobacterium tumefaciens | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Fulvimarina pelagi | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Oceanicola granulosus | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Oceanicola granulosus DSM 15982 | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Streptomyces viridochromogenes DSM 40736 | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Pseudomonas syringae pv. tomato DC3000 | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Streptomyces viridochromogenes | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Pseudomonas syringae pv. tomato | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Agrobacterium tumefaciens | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Fulvimarina pelagi | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Oceanicola granulosus | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Oceanicola granulosus DSM 15982 | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Streptomyces viridochromogenes DSM 40736 | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Pseudomonas syringae pv. tomato DC3000 | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Streptomyces viridochromogenes | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Pseudomonas syringae pv. tomato | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Agrobacterium tumefaciens | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Fulvimarina pelagi | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Oceanicola granulosus | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Oceanicola granulosus DSM 15982 | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Streptomyces viridochromogenes DSM 40736 | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Pseudomonas syringae pv. tomato DC3000 | ? + NADH + H+ | - |
? | |
D-mannuronate + NAD+ | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | ? + NADH + H+ | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Streptomyces viridochromogenes | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Pseudomonas syringae pv. tomato | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Agrobacterium tumefaciens | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Fulvimarina pelagi | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Oceanicola granulosus | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Oceanicola granulosus DSM 15982 | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Streptomyces viridochromogenes DSM 40736 | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Pseudomonas syringae pv. tomato DC3000 | ? | - |
? | |
additional information | substrate and cofactor specificity of Udhs, overview | Agrobacterium tumefaciens C58 / ATCC 33970 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Atu3143 | - |
Agrobacterium tumefaciens |
PSPTO_1053 | - |
Pseudomonas syringae pv. tomato |
Udh | - |
Streptomyces viridochromogenes |
Udh | - |
Pseudomonas syringae pv. tomato |
Udh | - |
Agrobacterium tumefaciens |
Udh | - |
Fulvimarina pelagi |
Udh | - |
Oceanicola granulosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Oceanicola granulosus |
35 | - |
- |
Pseudomonas syringae pv. tomato |
45 | - |
- |
Streptomyces viridochromogenes |
60 | - |
- |
Agrobacterium tumefaciens |
60 | - |
- |
Fulvimarina pelagi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | activity range, profile overview | Oceanicola granulosus |
15 | 60 | activity is constantly increasing within this range, profile overview | Agrobacterium tumefaciens |
15 | 60 | activity is constantly increasing within this range, profile overview | Fulvimarina pelagi |
15 | 50 | activity range, inactivation at 55°C, profile overview | Pseudomonas syringae pv. tomato |
15 | 55 | activity range, profile overview | Streptomyces viridochromogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified recombinant His-tagged enzyme, 10% activity remaning after 100 min, inactivation within 150 min | Agrobacterium tumefaciens |
37 | - |
purified recombinant His-tagged enzyme, 50% activity remaining after 120 min, 25% activity remaining after 300 min | Fulvimarina pelagi |
37 | - |
purified recombinant His-tagged enzyme, 50% remaining activity after 1000-1500 min, 30% remaning after 2500 min | Oceanicola granulosus |
37 | - |
purified recombinant His-tagged enzyme, inactivation within 50 min | Streptomyces viridochromogenes |
37 | - |
purified recombinant His-tagged enzyme, inactivation within 50 min | Pseudomonas syringae pv. tomato |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
3.4 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
4 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
4 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
6 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
6.4 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
7 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
7 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
7 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
8 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
9 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
10 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
13 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
17 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
21 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
23 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
29 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
53 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
58 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
59 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
62 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
63 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
75 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
76 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
78 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
82 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
85 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
92 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
107 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
108 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
113 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
144 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
171 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
215 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
333 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Oceanicola granulosus |
8 | - |
- |
Pseudomonas syringae pv. tomato |
8 | 9 | - |
Agrobacterium tumefaciens |
9 | - |
- |
Fulvimarina pelagi |
9.5 | 10 | - |
Streptomyces viridochromogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, profile overview | Agrobacterium tumefaciens |
5 | 10 | activity range, profile overview | Fulvimarina pelagi |
5 | 10 | activity is constantly increasing in this range, profile overview | Streptomyces viridochromogenes |
5.5 | 9.5 | activity range, profile overview | Pseudomonas syringae pv. tomato |
5.5 | 9.5 | activity range, profile overview | Oceanicola granulosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptomyces viridochromogenes | |
NAD+ | - |
Pseudomonas syringae pv. tomato | |
NAD+ | - |
Agrobacterium tumefaciens | |
NAD+ | - |
Fulvimarina pelagi | |
NAD+ | - |
Oceanicola granulosus | |
NADH | - |
Streptomyces viridochromogenes | |
NADH | - |
Pseudomonas syringae pv. tomato | |
NADH | - |
Agrobacterium tumefaciens | |
NADH | - |
Fulvimarina pelagi | |
NADH | - |
Oceanicola granulosus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily | Streptomyces viridochromogenes |
evolution | the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily | Pseudomonas syringae pv. tomato |
evolution | the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily | Agrobacterium tumefaciens |
evolution | the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily | Fulvimarina pelagi |
evolution | the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily | Oceanicola granulosus |
metabolism | uronate dehydrogenases catalyse the initial step in an oxidative pathway | Streptomyces viridochromogenes |
metabolism | uronate dehydrogenases catalyse the initial step in an oxidative pathway | Pseudomonas syringae pv. tomato |
metabolism | uronate dehydrogenases catalyse the initial step in an oxidative pathway | Agrobacterium tumefaciens |
metabolism | uronate dehydrogenases catalyse the initial step in an oxidative pathway | Oceanicola granulosus |
metabolism | uronate dehydrogenases catalyses the initial step in an oxidative pathway | Fulvimarina pelagi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
0.03 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
0.04 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
0.07 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
0.1 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Oceanicola granulosus | |
0.11 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Oceanicola granulosus | |
0.3 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
0.7 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
0.7 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
4 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
9 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
11 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
11 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
12 | - |
D-Mannuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
88 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
98 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
200 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
201 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Streptomyces viridochromogenes | |
210 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
257 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
269 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
274 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
342 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
406 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
430 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Fulvimarina pelagi | |
465 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
628 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Pseudomonas syringae pv. tomato | |
638 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Streptomyces viridochromogenes | |
646 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
785 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Fulvimarina pelagi | |
794 | - |
beta-D-galacturonate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato | |
829 | - |
beta-D-glucuronate | recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer | Agrobacterium tumefaciens | |
1083 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Agrobacterium tumefaciens | |
1262 | - |
NAD+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer | Pseudomonas syringae pv. tomato |