Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.203 extracted from

  • Wagschal, K.; Jordan, D.B.; Lee, C.C.; Younger, A.; Braker, J.D.; Chan, V.J.
    Biochemical characterization of uronate dehydrogenases from three pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans (2015), Enzyme Microb. Technol., 69, 62-68.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas fluorescens
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas mendocina
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Chromohalobacter salexigens
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Polaromonas naphthalenivorans
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas syringae pv. tomato

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme structure, PDB ID 3AY3 Chromohalobacter salexigens

Inhibitors

Inhibitors Comment Organism Structure
D-galactaro-1,5-lactone noncompetitve versus NAD+ and beta-D-glucuronate Polaromonas naphthalenivorans
additional information inhibition mechanisms, overview Polaromonas naphthalenivorans
NADH competitve versus NAD+, noncompetitive versus beta-D-glucuronate Polaromonas naphthalenivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics and thermodynamics Pseudomonas fluorescens
additional information
-
additional information Michaelis-Menten kinetics and thermodynamics Pseudomonas mendocina
additional information
-
additional information Michaelis-Menten kinetics and thermodynamics Pseudomonas syringae pv. tomato
additional information
-
additional information steady-state and stopped-flow kinetics, Michaelis-Menten kinetics, and thermodynamics Polaromonas naphthalenivorans
0.053
-
beta-D-galacturonate pH 8.0, 25°C, method A Pseudomonas mendocina
0.06
-
NAD+ pH 8.0, 25°C, method B Polaromonas naphthalenivorans
0.078
-
NAD+ pH 8.0, 25°C, method B Pseudomonas mendocina
0.106
-
beta-D-galacturonate pH 8.0, 25°C Pseudomonas fluorescens
0.107
-
beta-D-galacturonate pH 8.0, 25°C Pseudomonas syringae pv. tomato
0.138
-
beta-D-galacturonate pH 8.0, 25°C, method B Pseudomonas mendocina
0.148
-
beta-D-galacturonate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
0.16
-
beta-D-galacturonate pH 8.0, 25°C Chromohalobacter salexigens
0.24
-
NAD+ pH 8.0, 25°C Chromohalobacter salexigens
0.25
-
NAD+ pH 8.0, 25°C Pseudomonas fluorescens
0.25
-
NAD+ pH 8.0, 25°C, method A Pseudomonas mendocina
0.279
-
beta-D-galacturonate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
0.37
-
NAD+ pH 8.0, 25°C Pseudomonas syringae pv. tomato
0.4
-
NAD+ pH 8.0, 25°C, method A Polaromonas naphthalenivorans
0.48
-
beta-D-glucuronate pH 8.0, 25°C, method A Pseudomonas mendocina
0.69
-
beta-D-glucuronate pH 8.0, 25°C Pseudomonas fluorescens
1.08
-
beta-D-glucuronate pH 8.0, 25°C, method B Pseudomonas mendocina
1.27
-
beta-D-glucuronate pH 8.0, 25°C Chromohalobacter salexigens
1.3
-
beta-D-glucuronate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
1.34
-
beta-D-glucuronate pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.94
-
beta-D-glucuronate pH 8.0, 25°C, method B Polaromonas naphthalenivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-galacturonate + NAD+ Pseudomonas fluorescens
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Pseudomonas mendocina
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Chromohalobacter salexigens
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Polaromonas naphthalenivorans
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Pseudomonas syringae pv. tomato
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Pseudomonas fluorescens Pf0-1
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Pseudomonas fluorescens
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Pseudomonas mendocina
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Chromohalobacter salexigens
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Polaromonas naphthalenivorans
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Pseudomonas syringae pv. tomato
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Pseudomonas fluorescens Pf0-1
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000
-
D-glucaro-1,5-lactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens
-
-
-
Polaromonas naphthalenivorans
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens Pf0-1
-
-
-
Pseudomonas mendocina
-
-
-
Pseudomonas syringae pv. tomato Q888H1
-
-
Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 Q888H1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas fluorescens
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas mendocina
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Chromohalobacter salexigens
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Polaromonas naphthalenivorans
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas syringae pv. tomato

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galacturonate + NAD+
-
Pseudomonas fluorescens D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Pseudomonas mendocina D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Chromohalobacter salexigens D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Polaromonas naphthalenivorans D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Pseudomonas syringae pv. tomato D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Pseudomonas fluorescens Pf0-1 D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Pseudomonas fluorescens D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Pseudomonas mendocina D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Chromohalobacter salexigens D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Polaromonas naphthalenivorans D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Pseudomonas syringae pv. tomato D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Pseudomonas fluorescens Pf0-1 D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 D-glucaro-1,5-lactone + NADH + H+
-
?

Subunits

Subunits Comment Organism
More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Pseudomonas fluorescens
More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Pseudomonas mendocina
More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Chromohalobacter salexigens
More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Polaromonas naphthalenivorans
More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Pseudomonas syringae pv. tomato

Synonyms

Synonyms Comment Organism
CsUDH
-
Chromohalobacter salexigens
PfUDH
-
Pseudomonas fluorescens
PmUDH
-
Pseudomonas mendocina
PnUDH
-
Polaromonas naphthalenivorans
PSPTO_1053
-
Pseudomonas syringae pv. tomato
PsUDH
-
Pseudomonas syringae pv. tomato
Udh
-
Pseudomonas fluorescens
Udh
-
Pseudomonas mendocina
Udh
-
Chromohalobacter salexigens
Udh
-
Polaromonas naphthalenivorans
Udh
-
Pseudomonas syringae pv. tomato

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas fluorescens
25
-
assay at Pseudomonas mendocina
25
-
assay at Chromohalobacter salexigens
25
-
assay at Polaromonas naphthalenivorans
25
-
assay at Pseudomonas syringae pv. tomato

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.3
-
beta-D-galacturonate pH 8.0, 25°C Chromohalobacter salexigens
17.1
-
beta-D-galacturonate pH 8.0, 25°C Pseudomonas fluorescens
17.4
-
beta-D-galacturonate pH 8.0, 25°C, method A Pseudomonas mendocina
17.9
-
beta-D-galacturonate pH 8.0, 25°C, method B Pseudomonas mendocina
27.8
-
beta-D-galacturonate pH 8.0, 25°C Pseudomonas syringae pv. tomato
33.8
-
beta-D-galacturonate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
51.6
-
beta-D-galacturonate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
101
-
beta-D-glucuronate pH 8.0, 25°C, method B Pseudomonas mendocina
119
-
beta-D-glucuronate pH 8.0, 25°C Chromohalobacter salexigens
152
-
beta-D-glucuronate pH 8.0, 25°C Pseudomonas fluorescens
159
-
beta-D-glucuronate pH 8.0, 25°C, method A Pseudomonas mendocina
212
-
beta-D-glucuronate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
217
-
beta-D-glucuronate pH 8.0, 25°C Pseudomonas syringae pv. tomato
274
-
beta-D-glucuronate pH 8.0, 25°C, method A Polaromonas naphthalenivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas fluorescens
8
-
assay at Pseudomonas mendocina
8
-
assay at Chromohalobacter salexigens
8
-
assay at Polaromonas naphthalenivorans
8
-
assay at Pseudomonas syringae pv. tomato

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas fluorescens
NAD+
-
Pseudomonas mendocina
NAD+
-
Chromohalobacter salexigens
NAD+
-
Pseudomonas syringae pv. tomato
NAD+ binding kinetics, overview Polaromonas naphthalenivorans
NADH
-
Pseudomonas fluorescens
NADH
-
Pseudomonas mendocina
NADH
-
Chromohalobacter salexigens
NADH
-
Polaromonas naphthalenivorans
NADH
-
Pseudomonas syringae pv. tomato

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics, overview Polaromonas naphthalenivorans

General Information

General Information Comment Organism
evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Pseudomonas fluorescens
evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Pseudomonas mendocina
evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Chromohalobacter salexigens
evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Polaromonas naphthalenivorans
evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Pseudomonas syringae pv. tomato

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics and thermodynamics Chromohalobacter salexigens
93.5
-
beta-D-glucuronate pH 8.0, 25°C, method B Pseudomonas mendocina
93.7
-
beta-D-glucuronate pH 8.0, 25°C Chromohalobacter salexigens
96
-
beta-D-galacturonate pH 8.0, 25°C Chromohalobacter salexigens
109
-
beta-D-glucuronate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
130
-
beta-D-galacturonate pH 8.0, 25°C, method B Pseudomonas mendocina
161
-
beta-D-galacturonate pH 8.0, 25°C Pseudomonas fluorescens
162
-
beta-D-glucuronate pH 8.0, 25°C Pseudomonas syringae pv. tomato
185
-
beta-D-galacturonate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
211
-
beta-D-glucuronate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
220
-
beta-D-glucuronate pH 8.0, 25°C Pseudomonas fluorescens
228
-
beta-D-galacturonate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
260
-
beta-D-galacturonate pH 8.0, 25°C Pseudomonas syringae pv. tomato
328
-
beta-D-galacturonate pH 8.0, 25°C, method A Pseudomonas mendocina
331
-
beta-D-glucuronate pH 8.0, 25°C, method A Pseudomonas mendocina