Cloned (Comment) | Organism |
---|---|
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas fluorescens |
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas mendocina |
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Chromohalobacter salexigens |
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Polaromonas naphthalenivorans |
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas syringae pv. tomato |
Crystallization (Comment) | Organism |
---|---|
enzyme structure, PDB ID 3AY3 | Chromohalobacter salexigens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactaro-1,5-lactone | noncompetitve versus NAD+ and beta-D-glucuronate | Polaromonas naphthalenivorans | |
additional information | inhibition mechanisms, overview | Polaromonas naphthalenivorans | |
NADH | competitve versus NAD+, noncompetitive versus beta-D-glucuronate | Polaromonas naphthalenivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics and thermodynamics | Pseudomonas fluorescens | |
additional information | - |
additional information | Michaelis-Menten kinetics and thermodynamics | Pseudomonas mendocina | |
additional information | - |
additional information | Michaelis-Menten kinetics and thermodynamics | Pseudomonas syringae pv. tomato | |
additional information | - |
additional information | steady-state and stopped-flow kinetics, Michaelis-Menten kinetics, and thermodynamics | Polaromonas naphthalenivorans | |
0.053 | - |
beta-D-galacturonate | pH 8.0, 25°C, method A | Pseudomonas mendocina | |
0.06 | - |
NAD+ | pH 8.0, 25°C, method B | Polaromonas naphthalenivorans | |
0.078 | - |
NAD+ | pH 8.0, 25°C, method B | Pseudomonas mendocina | |
0.106 | - |
beta-D-galacturonate | pH 8.0, 25°C | Pseudomonas fluorescens | |
0.107 | - |
beta-D-galacturonate | pH 8.0, 25°C | Pseudomonas syringae pv. tomato | |
0.138 | - |
beta-D-galacturonate | pH 8.0, 25°C, method B | Pseudomonas mendocina | |
0.148 | - |
beta-D-galacturonate | pH 8.0, 25°C, method A | Polaromonas naphthalenivorans | |
0.16 | - |
beta-D-galacturonate | pH 8.0, 25°C | Chromohalobacter salexigens | |
0.24 | - |
NAD+ | pH 8.0, 25°C | Chromohalobacter salexigens | |
0.25 | - |
NAD+ | pH 8.0, 25°C | Pseudomonas fluorescens | |
0.25 | - |
NAD+ | pH 8.0, 25°C, method A | Pseudomonas mendocina | |
0.279 | - |
beta-D-galacturonate | pH 8.0, 25°C, method B | Polaromonas naphthalenivorans | |
0.37 | - |
NAD+ | pH 8.0, 25°C | Pseudomonas syringae pv. tomato | |
0.4 | - |
NAD+ | pH 8.0, 25°C, method A | Polaromonas naphthalenivorans | |
0.48 | - |
beta-D-glucuronate | pH 8.0, 25°C, method A | Pseudomonas mendocina | |
0.69 | - |
beta-D-glucuronate | pH 8.0, 25°C | Pseudomonas fluorescens | |
1.08 | - |
beta-D-glucuronate | pH 8.0, 25°C, method B | Pseudomonas mendocina | |
1.27 | - |
beta-D-glucuronate | pH 8.0, 25°C | Chromohalobacter salexigens | |
1.3 | - |
beta-D-glucuronate | pH 8.0, 25°C, method A | Polaromonas naphthalenivorans | |
1.34 | - |
beta-D-glucuronate | pH 8.0, 25°C | Pseudomonas syringae pv. tomato | |
1.94 | - |
beta-D-glucuronate | pH 8.0, 25°C, method B | Polaromonas naphthalenivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galacturonate + NAD+ | Pseudomonas fluorescens | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Pseudomonas mendocina | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Chromohalobacter salexigens | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Polaromonas naphthalenivorans | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Pseudomonas syringae pv. tomato | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Pseudomonas fluorescens Pf0-1 | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Pseudomonas fluorescens | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Pseudomonas mendocina | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Chromohalobacter salexigens | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Polaromonas naphthalenivorans | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Pseudomonas syringae pv. tomato | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Pseudomonas fluorescens Pf0-1 | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromohalobacter salexigens | - |
- |
- |
Polaromonas naphthalenivorans | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens Pf0-1 | - |
- |
- |
Pseudomonas mendocina | - |
- |
- |
Pseudomonas syringae pv. tomato | Q888H1 | - |
- |
Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 | Q888H1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Pseudomonas fluorescens |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Pseudomonas mendocina |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Chromohalobacter salexigens |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Polaromonas naphthalenivorans |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Pseudomonas syringae pv. tomato |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galacturonate + NAD+ | - |
Pseudomonas fluorescens | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Pseudomonas mendocina | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Chromohalobacter salexigens | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Polaromonas naphthalenivorans | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Pseudomonas syringae pv. tomato | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Pseudomonas fluorescens Pf0-1 | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-galacturonate + NAD+ | - |
Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Pseudomonas fluorescens | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Pseudomonas mendocina | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Chromohalobacter salexigens | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Polaromonas naphthalenivorans | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Pseudomonas syringae pv. tomato | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Pseudomonas fluorescens Pf0-1 | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 | D-glucaro-1,5-lactone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview | Pseudomonas fluorescens |
More | amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview | Pseudomonas mendocina |
More | amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview | Chromohalobacter salexigens |
More | amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview | Polaromonas naphthalenivorans |
More | amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview | Pseudomonas syringae pv. tomato |
Synonyms | Comment | Organism |
---|---|---|
CsUDH | - |
Chromohalobacter salexigens |
PfUDH | - |
Pseudomonas fluorescens |
PmUDH | - |
Pseudomonas mendocina |
PnUDH | - |
Polaromonas naphthalenivorans |
PSPTO_1053 | - |
Pseudomonas syringae pv. tomato |
PsUDH | - |
Pseudomonas syringae pv. tomato |
Udh | - |
Pseudomonas fluorescens |
Udh | - |
Pseudomonas mendocina |
Udh | - |
Chromohalobacter salexigens |
Udh | - |
Polaromonas naphthalenivorans |
Udh | - |
Pseudomonas syringae pv. tomato |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
25 | - |
assay at | Pseudomonas mendocina |
25 | - |
assay at | Chromohalobacter salexigens |
25 | - |
assay at | Polaromonas naphthalenivorans |
25 | - |
assay at | Pseudomonas syringae pv. tomato |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.3 | - |
beta-D-galacturonate | pH 8.0, 25°C | Chromohalobacter salexigens | |
17.1 | - |
beta-D-galacturonate | pH 8.0, 25°C | Pseudomonas fluorescens | |
17.4 | - |
beta-D-galacturonate | pH 8.0, 25°C, method A | Pseudomonas mendocina | |
17.9 | - |
beta-D-galacturonate | pH 8.0, 25°C, method B | Pseudomonas mendocina | |
27.8 | - |
beta-D-galacturonate | pH 8.0, 25°C | Pseudomonas syringae pv. tomato | |
33.8 | - |
beta-D-galacturonate | pH 8.0, 25°C, method A | Polaromonas naphthalenivorans | |
51.6 | - |
beta-D-galacturonate | pH 8.0, 25°C, method B | Polaromonas naphthalenivorans | |
101 | - |
beta-D-glucuronate | pH 8.0, 25°C, method B | Pseudomonas mendocina | |
119 | - |
beta-D-glucuronate | pH 8.0, 25°C | Chromohalobacter salexigens | |
152 | - |
beta-D-glucuronate | pH 8.0, 25°C | Pseudomonas fluorescens | |
159 | - |
beta-D-glucuronate | pH 8.0, 25°C, method A | Pseudomonas mendocina | |
212 | - |
beta-D-glucuronate | pH 8.0, 25°C, method B | Polaromonas naphthalenivorans | |
217 | - |
beta-D-glucuronate | pH 8.0, 25°C | Pseudomonas syringae pv. tomato | |
274 | - |
beta-D-glucuronate | pH 8.0, 25°C, method A | Polaromonas naphthalenivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas fluorescens |
8 | - |
assay at | Pseudomonas mendocina |
8 | - |
assay at | Chromohalobacter salexigens |
8 | - |
assay at | Polaromonas naphthalenivorans |
8 | - |
assay at | Pseudomonas syringae pv. tomato |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas fluorescens | |
NAD+ | - |
Pseudomonas mendocina | |
NAD+ | - |
Chromohalobacter salexigens | |
NAD+ | - |
Pseudomonas syringae pv. tomato | |
NAD+ | binding kinetics, overview | Polaromonas naphthalenivorans | |
NADH | - |
Pseudomonas fluorescens | |
NADH | - |
Pseudomonas mendocina | |
NADH | - |
Chromohalobacter salexigens | |
NADH | - |
Polaromonas naphthalenivorans | |
NADH | - |
Pseudomonas syringae pv. tomato |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Polaromonas naphthalenivorans |
General Information | Comment | Organism |
---|---|---|
evolution | UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site | Pseudomonas fluorescens |
evolution | UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site | Pseudomonas mendocina |
evolution | UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site | Chromohalobacter salexigens |
evolution | UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site | Polaromonas naphthalenivorans |
evolution | UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site | Pseudomonas syringae pv. tomato |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics and thermodynamics | Chromohalobacter salexigens | |
93.5 | - |
beta-D-glucuronate | pH 8.0, 25°C, method B | Pseudomonas mendocina | |
93.7 | - |
beta-D-glucuronate | pH 8.0, 25°C | Chromohalobacter salexigens | |
96 | - |
beta-D-galacturonate | pH 8.0, 25°C | Chromohalobacter salexigens | |
109 | - |
beta-D-glucuronate | pH 8.0, 25°C, method B | Polaromonas naphthalenivorans | |
130 | - |
beta-D-galacturonate | pH 8.0, 25°C, method B | Pseudomonas mendocina | |
161 | - |
beta-D-galacturonate | pH 8.0, 25°C | Pseudomonas fluorescens | |
162 | - |
beta-D-glucuronate | pH 8.0, 25°C | Pseudomonas syringae pv. tomato | |
185 | - |
beta-D-galacturonate | pH 8.0, 25°C, method B | Polaromonas naphthalenivorans | |
211 | - |
beta-D-glucuronate | pH 8.0, 25°C, method A | Polaromonas naphthalenivorans | |
220 | - |
beta-D-glucuronate | pH 8.0, 25°C | Pseudomonas fluorescens | |
228 | - |
beta-D-galacturonate | pH 8.0, 25°C, method A | Polaromonas naphthalenivorans | |
260 | - |
beta-D-galacturonate | pH 8.0, 25°C | Pseudomonas syringae pv. tomato | |
328 | - |
beta-D-galacturonate | pH 8.0, 25°C, method A | Pseudomonas mendocina | |
331 | - |
beta-D-glucuronate | pH 8.0, 25°C, method A | Pseudomonas mendocina |