BRENDA - Enzyme Database show
show all sequences of 1.1.1.203

Cloning and characterization of uronate dehydrogenases from two pseudomonads and Agrobacterium tumefaciens strain C58

Yoon, S.H.; Moon, T.S.; Iranpour, P.; Lanza, A.M.; Prather, K.J.; J. Bacteriol. 191, 1565-1573 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC
Agrobacterium tumefaciens
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC
Pseudomonas putida KT2440
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC
Pseudomonas syringae pv. tomato
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
D-galacturonate
-
Pseudomonas syringae pv. tomato
0.1
-
D-galacturonate
-
Pseudomonas putida KT2440
0.16
-
D-galacturonate
-
Agrobacterium tumefaciens
0.17
-
NAD+
-
Pseudomonas syringae pv. tomato
0.18
-
NAD+
-
Agrobacterium tumefaciens
0.21
-
NAD+
-
Pseudomonas putida KT2440
0.25
-
D-glucuronate
-
Pseudomonas putida KT2440
0.28
-
D-glucuronate
-
Pseudomonas syringae pv. tomato
0.37
-
D-glucuronate
-
Agrobacterium tumefaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Pseudomonas putida KT2440
30000
-
x * 30000, SDS-PAGE
Pseudomonas syringae pv. tomato
32000
-
x * 32000, SDS-PAGE
Agrobacterium tumefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
Q7CRQ0
strain C58
-
Agrobacterium tumefaciens C58
Q7CRQ0
strain C58
-
Pseudomonas putida KT2440
Q88NN6
-
-
Pseudomonas syringae pv. tomato
Q888H1
-
-
Purification (Commentary)
Commentary
Organism
gel filtration
Agrobacterium tumefaciens
gel filtration
Pseudomonas putida KT2440
gel filtration
Pseudomonas syringae pv. tomato
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NAD+ + H2O
-
698607
Pseudomonas syringae pv. tomato
D-galactarate + NADH + H+
-
-
-
?
D-galacturonate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens
D-galactarate + NADH + H+
-
-
-
?
D-galacturonate + NAD+ + H2O
-
698607
Pseudomonas putida KT2440
D-galactarate + NADH + H+
-
-
-
?
D-galacturonate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens C58
D-galactarate + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Pseudomonas syringae pv. tomato
D-glucaric acid + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens
D-glucaric acid + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Pseudomonas putida KT2440
D-glucaric acid + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens C58
D-glucaric acid + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Agrobacterium tumefaciens
?
x * 30000, SDS-PAGE
Pseudomonas putida KT2440
?
x * 30000, SDS-PAGE
Pseudomonas syringae pv. tomato
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.24
-
D-galacturonate
-
Pseudomonas syringae pv. tomato
0.3
-
D-galacturonate
-
Pseudomonas putida KT2440
0.55
-
D-glucuronate
-
Pseudomonas putida KT2440
0.74
-
D-glucuronate
-
Pseudomonas syringae pv. tomato
0.92
-
D-galacturonate
-
Agrobacterium tumefaciens
1.9
-
D-glucuronate
-
Agrobacterium tumefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas putida KT2440
8
-
-
Agrobacterium tumefaciens
8
-
-
Pseudomonas syringae pv. tomato
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida KT2440
NAD+
-
Pseudomonas syringae pv. tomato
NAD+
-
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC
Agrobacterium tumefaciens
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC
Pseudomonas putida KT2440
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC
Pseudomonas syringae pv. tomato
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida KT2440
NAD+
-
Pseudomonas syringae pv. tomato
NAD+
-
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
D-galacturonate
-
Pseudomonas syringae pv. tomato
0.1
-
D-galacturonate
-
Pseudomonas putida KT2440
0.16
-
D-galacturonate
-
Agrobacterium tumefaciens
0.17
-
NAD+
-
Pseudomonas syringae pv. tomato
0.18
-
NAD+
-
Agrobacterium tumefaciens
0.21
-
NAD+
-
Pseudomonas putida KT2440
0.25
-
D-glucuronate
-
Pseudomonas putida KT2440
0.28
-
D-glucuronate
-
Pseudomonas syringae pv. tomato
0.37
-
D-glucuronate
-
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Pseudomonas putida KT2440
30000
-
x * 30000, SDS-PAGE
Pseudomonas syringae pv. tomato
32000
-
x * 32000, SDS-PAGE
Agrobacterium tumefaciens
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration
Agrobacterium tumefaciens
gel filtration
Pseudomonas putida KT2440
gel filtration
Pseudomonas syringae pv. tomato
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NAD+ + H2O
-
698607
Pseudomonas syringae pv. tomato
D-galactarate + NADH + H+
-
-
-
?
D-galacturonate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens
D-galactarate + NADH + H+
-
-
-
?
D-galacturonate + NAD+ + H2O
-
698607
Pseudomonas putida KT2440
D-galactarate + NADH + H+
-
-
-
?
D-galacturonate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens C58
D-galactarate + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Pseudomonas syringae pv. tomato
D-glucaric acid + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens
D-glucaric acid + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Pseudomonas putida KT2440
D-glucaric acid + NADH + H+
-
-
-
?
D-glucuronate + NAD+ + H2O
-
698607
Agrobacterium tumefaciens C58
D-glucaric acid + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Agrobacterium tumefaciens
?
x * 30000, SDS-PAGE
Pseudomonas putida KT2440
?
x * 30000, SDS-PAGE
Pseudomonas syringae pv. tomato
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.24
-
D-galacturonate
-
Pseudomonas syringae pv. tomato
0.3
-
D-galacturonate
-
Pseudomonas putida KT2440
0.55
-
D-glucuronate
-
Pseudomonas putida KT2440
0.74
-
D-glucuronate
-
Pseudomonas syringae pv. tomato
0.92
-
D-galacturonate
-
Agrobacterium tumefaciens
1.9
-
D-glucuronate
-
Agrobacterium tumefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas putida KT2440
8
-
-
Agrobacterium tumefaciens
8
-
-
Pseudomonas syringae pv. tomato
Other publictions for EC 1.1.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725435
Parkkinen
Crystal structure of uronate d ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
J. Biol. Chem.
286
27294-27300
2011
-
-
1
1
1
-
-
-
-
-
-
4
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695552
Moon
Enzymatic assay of D-glucurona ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
Anal. Biochem.
392
183-185
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
20
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695783
Moon
Production of glucaric acid fr ...
Pseudomonas syringae pv. tomato
Appl. Environ. Microbiol.
75
589-595
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
698607
Yoon
Cloning and characterization o ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58, Pseudomonas putida KT2440, Pseudomonas syringae pv. tomato
J. Bacteriol.
191
1565-1573
2009
-
-
3
-
-
-
-
9
-
-
3
-
-
13
-
-
3
-
-
-
-
-
8
3
-
-
-
6
3
-
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
9
-
-
3
-
-
-
-
3
-
-
-
-
8
3
-
-
-
6
3
-
-
-
-
-
-
-
-
-
286231
Wagner
Uronic acid dehydrogenase from ...
Pseudomonas syringae
Eur. J. Biochem.
61
589-596
1976
-
-
-
-
-
1
4
3
-
-
2
2
-
2
-
-
1
-
1
1
1
2
3
1
1
-
3
2
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
3
-
-
2
2
-
-
-
1
1
1
1
2
3
1
1
-
3
2
-
-
-
-
-
-
-
-
-
-
286230
Bateman
Purification and properties of ...
Pseudomonas syringae
Arch. Biochem. Biophys.
136
97-105
1970
-
-
-
-
-
1
1
3
-
-
-
2
-
2
-
-
1
-
-
1
1
1
2
-
-
-
2
-
1
-
2
1
-
-
-
-
-
-
1
-
-
1
-
1
-
3
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-