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Literature summary for 1.1.1.203 extracted from

  • Wagner, G.; Hollmann, S.
    Uronic acid dehydrogenase from Pseudomonas syringae. Purification and properties (1976), Eur. J. Biochem., 61, 589-596.
    View publication on PubMed

General Stability

General Stability Organism
DTT and dithioerythritol have protective function against thermal destruction Pseudomonas syringae

Inhibitors

Inhibitors Comment Organism Structure
KCN 15% inhibition at 1 mM Pseudomonas syringae
N-ethylmaleimide inhibition at 1 mM, reversal by an excess of L-cysteine Pseudomonas syringae
NADH competitive inhibition, Ki: 0.06 mM Pseudomonas syringae
p-chloromercuribenzoic acid 60% inhibition at 1 mM, reversal by an excess of L-cysteine Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
D-galacturonate
-
Pseudomonas syringae
0.08
-
NAD+
-
Pseudomonas syringae
0.37
-
D-glucuronate
-
Pseudomonas syringae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
2 * 30500, SDS-PAGE, subunits alone inactive Pseudomonas syringae
59000
-
gel filtration, ultracentrifugation Pseudomonas syringae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucuronate + NAD+ Pseudomonas syringae weak reversible reaction, 3% of the activity compared to the forward reaction D-glucaric acid 3,6-lactone + NADH + H+
-
r
D-glucuronate + NAD+ + H2O Pseudomonas syringae
-
D-glucaric acid + NADH + H+
-
ir

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Pseudomonas syringae

Renatured (Commentary)

Renatured (Comment) Organism
treatment with 8 M urea and 24 h dialysis: 10% of initial activity Pseudomonas syringae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas syringae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81
-
-
Pseudomonas syringae

Storage Stability

Storage Stability Organism
-20°C, 50 mM sodium diphosphate-HCl, pH 8.0, 0.1% bovine serum albumin, no loss of activity Pseudomonas syringae
4°C, 50 mM sodium diphosphate-HCl, pH 8.0, 30% glycerol, 5% loss of activity within 1 month Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NAD+ + H2O
-
Pseudomonas syringae D-galactarate + NADH + H+
-
?
D-glucuronate + NAD+ weak reversible reaction, 3% of the activity compared to the forward reaction Pseudomonas syringae D-glucaric acid 3,6-lactone + NADH + H+
-
r
D-glucuronate + NAD+ + H2O
-
Pseudomonas syringae D-glucaric acid + NADH + H+
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 30500, SDS-PAGE, subunits alone inactive Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Pseudomonas syringae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
15 stable for 24 h Pseudomonas syringae
25
-
15% loss of activity within 2 h Pseudomonas syringae
37
-
90% loss of activity within 60 min Pseudomonas syringae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.7
-
D-galacturonate
-
Pseudomonas syringae
80
-
D-glucuronate
-
Pseudomonas syringae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas syringae