BRENDA - Enzyme Database show
show all sequences of 1.1.1.203

Uronic acid dehydrogenase from Pseudomonas syringae. Purification and properties

Wagner, G.; Hollmann, S.; Eur. J. Biochem. 61, 589-596 (1976)

Data extracted from this reference:

General Stability
General Stability
Organism
DTT and dithioerythritol have protective function against thermal destruction
Pseudomonas syringae
Inhibitors
Inhibitors
Commentary
Organism
Structure
KCN
15% inhibition at 1 mM
Pseudomonas syringae
N-ethylmaleimide
inhibition at 1 mM, reversal by an excess of L-cysteine
Pseudomonas syringae
NADH
competitive inhibition, Ki: 0.06 mM
Pseudomonas syringae
p-chloromercuribenzoic acid
60% inhibition at 1 mM, reversal by an excess of L-cysteine
Pseudomonas syringae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.054
-
D-galacturonate
-
Pseudomonas syringae
0.08
-
NAD+
-
Pseudomonas syringae
0.37
-
D-glucuronate
-
Pseudomonas syringae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30500
-
2 * 30500, SDS-PAGE, subunits alone inactive
Pseudomonas syringae
59000
-
gel filtration, ultracentrifugation
Pseudomonas syringae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucuronate + NAD+
Pseudomonas syringae
weak reversible reaction, 3% of the activity compared to the forward reaction
D-glucaric acid 3,6-lactone + NADH + H+
-
Pseudomonas syringae
r
D-glucuronate + NAD+ + H2O
Pseudomonas syringae
-
D-glucaric acid + NADH + H+
-
Pseudomonas syringae
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas syringae
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas syringae
Renatured (Commentary)
Commentary
Organism
treatment with 8 M urea and 24 h dialysis: 10% of initial activity
Pseudomonas syringae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas syringae
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
81
-
-
Pseudomonas syringae
Storage Stability
Storage Stability
Organism
-20°C, 50 mM sodium diphosphate-HCl, pH 8.0, 0.1% bovine serum albumin, no loss of activity
Pseudomonas syringae
4°C, 50 mM sodium diphosphate-HCl, pH 8.0, 30% glycerol, 5% loss of activity within 1 month
Pseudomonas syringae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NAD+ + H2O
-
286231
Pseudomonas syringae
D-galactarate + NADH + H+
-
-
-
-
D-glucuronate + NAD+
weak reversible reaction, 3% of the activity compared to the forward reaction
286231
Pseudomonas syringae
D-glucaric acid 3,6-lactone + NADH + H+
-
286231
Pseudomonas syringae
r
D-glucuronate + NAD+ + H2O
-
286231
Pseudomonas syringae
D-glucaric acid + NADH + H+
-
286231
Pseudomonas syringae
ir
Subunits
Subunits
Commentary
Organism
dimer
2 * 30500, SDS-PAGE, subunits alone inactive
Pseudomonas syringae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Pseudomonas syringae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
15
stable for 24 h
Pseudomonas syringae
25
-
15% loss of activity within 2 h
Pseudomonas syringae
37
-
90% loss of activity within 60 min
Pseudomonas syringae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
26.7
-
D-galacturonate
-
Pseudomonas syringae
80
-
D-glucuronate
-
Pseudomonas syringae
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas syringae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas syringae
General Stability (protein specific)
General Stability
Organism
DTT and dithioerythritol have protective function against thermal destruction
Pseudomonas syringae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
KCN
15% inhibition at 1 mM
Pseudomonas syringae
N-ethylmaleimide
inhibition at 1 mM, reversal by an excess of L-cysteine
Pseudomonas syringae
NADH
competitive inhibition, Ki: 0.06 mM
Pseudomonas syringae
p-chloromercuribenzoic acid
60% inhibition at 1 mM, reversal by an excess of L-cysteine
Pseudomonas syringae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.054
-
D-galacturonate
-
Pseudomonas syringae
0.08
-
NAD+
-
Pseudomonas syringae
0.37
-
D-glucuronate
-
Pseudomonas syringae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30500
-
2 * 30500, SDS-PAGE, subunits alone inactive
Pseudomonas syringae
59000
-
gel filtration, ultracentrifugation
Pseudomonas syringae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucuronate + NAD+
Pseudomonas syringae
weak reversible reaction, 3% of the activity compared to the forward reaction
D-glucaric acid 3,6-lactone + NADH + H+
-
Pseudomonas syringae
r
D-glucuronate + NAD+ + H2O
Pseudomonas syringae
-
D-glucaric acid + NADH + H+
-
Pseudomonas syringae
ir
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas syringae
Renatured (Commentary) (protein specific)
Commentary
Organism
treatment with 8 M urea and 24 h dialysis: 10% of initial activity
Pseudomonas syringae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas syringae
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
81
-
-
Pseudomonas syringae
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 50 mM sodium diphosphate-HCl, pH 8.0, 0.1% bovine serum albumin, no loss of activity
Pseudomonas syringae
4°C, 50 mM sodium diphosphate-HCl, pH 8.0, 30% glycerol, 5% loss of activity within 1 month
Pseudomonas syringae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NAD+ + H2O
-
286231
Pseudomonas syringae
D-galactarate + NADH + H+
-
-
-
-
D-glucuronate + NAD+
weak reversible reaction, 3% of the activity compared to the forward reaction
286231
Pseudomonas syringae
D-glucaric acid 3,6-lactone + NADH + H+
-
286231
Pseudomonas syringae
r
D-glucuronate + NAD+ + H2O
-
286231
Pseudomonas syringae
D-glucaric acid + NADH + H+
-
286231
Pseudomonas syringae
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 30500, SDS-PAGE, subunits alone inactive
Pseudomonas syringae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Pseudomonas syringae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
15
stable for 24 h
Pseudomonas syringae
25
-
15% loss of activity within 2 h
Pseudomonas syringae
37
-
90% loss of activity within 60 min
Pseudomonas syringae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
26.7
-
D-galacturonate
-
Pseudomonas syringae
80
-
D-glucuronate
-
Pseudomonas syringae
Other publictions for EC 1.1.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725435
Parkkinen
Crystal structure of uronate d ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
J. Biol. Chem.
286
27294-27300
2011
-
-
1
1
1
-
-
-
-
-
-
4
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695552
Moon
Enzymatic assay of D-glucurona ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
Anal. Biochem.
392
183-185
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
20
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695783
Moon
Production of glucaric acid fr ...
Pseudomonas syringae pv. tomato
Appl. Environ. Microbiol.
75
589-595
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
698607
Yoon
Cloning and characterization o ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58, Pseudomonas putida KT2440, Pseudomonas syringae pv. tomato
J. Bacteriol.
191
1565-1573
2009
-
-
3
-
-
-
-
9
-
-
3
-
-
13
-
-
3
-
-
-
-
-
8
3
-
-
-
6
3
-
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
9
-
-
3
-
-
-
-
3
-
-
-
-
8
3
-
-
-
6
3
-
-
-
-
-
-
-
-
-
286231
Wagner
Uronic acid dehydrogenase from ...
Pseudomonas syringae
Eur. J. Biochem.
61
589-596
1976
-
-
-
-
-
1
4
3
-
-
2
2
-
2
-
-
1
-
1
1
1
2
3
1
1
-
3
2
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
3
-
-
2
2
-
-
-
1
1
1
1
2
3
1
1
-
3
2
-
-
-
-
-
-
-
-
-
-
286230
Bateman
Purification and properties of ...
Pseudomonas syringae
Arch. Biochem. Biophys.
136
97-105
1970
-
-
-
-
-
1
1
3
-
-
-
2
-
2
-
-
1
-
-
1
1
1
2
-
-
-
2
-
1
-
2
1
-
-
-
-
-
-
1
-
-
1
-
1
-
3
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-