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show all sequences of 1.1.1.203

Purification and properties of uronate dehydrogenase from Pseudomonas syringae

Bateman, D.F.; Kosuge, T.; Kilgore, W.W.; Arch. Biochem. Biophys. 136, 97-105 (1970)

Data extracted from this reference:

General Stability
General Stability
Organism
NAD+ increases stability
Pseudomonas syringae
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition at 1 mM
Pseudomonas syringae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
D-galacturonate
-
Pseudomonas syringae
0.15
-
D-glucuronate
-
Pseudomonas syringae
5.3
-
NAD+
with 0.67 mM D-galacturonate
Pseudomonas syringae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NAD+ + H2O
Pseudomonas syringae
50% of the activity compared to D-glucoronate
D-galactarate + NADH + H+
-
Pseudomonas syringae
ir
D-glucuronate + NAD+ + H2O
Pseudomonas syringae
strong substrate specificity, best substrate
D-glucaric acid + NADH + H+
-
Pseudomonas syringae
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas syringae
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas syringae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas syringae
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.2
-
-
Pseudomonas syringae
Storage Stability
Storage Stability
Organism
-10C, 50 mM Tris-HCl, pH 7.2, 2.9 mM NAD+, less then 10% loss of activity up to 6 months
Pseudomonas syringae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NAD+ + H2O
50% of the activity compared to D-glucoronate
286230
Pseudomonas syringae
D-galactarate + NADH + H+
-
286230
Pseudomonas syringae
ir
D-glucuronate + NAD+ + H2O
strong substrate specificity, best substrate
286230
Pseudomonas syringae
D-glucaric acid + NADH + H+
-
286230
Pseudomonas syringae
ir
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
stable
Pseudomonas syringae
30
35
loss of activity after 30 min
Pseudomonas syringae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8.4
-
Pseudomonas syringae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
50% remaining activity
Pseudomonas syringae
9.4
-
50% remaining activity
Pseudomonas syringae
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Pseudomonas syringae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Pseudomonas syringae
General Stability (protein specific)
General Stability
Organism
NAD+ increases stability
Pseudomonas syringae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition at 1 mM
Pseudomonas syringae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
D-galacturonate
-
Pseudomonas syringae
0.15
-
D-glucuronate
-
Pseudomonas syringae
5.3
-
NAD+
with 0.67 mM D-galacturonate
Pseudomonas syringae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NAD+ + H2O
Pseudomonas syringae
50% of the activity compared to D-glucoronate
D-galactarate + NADH + H+
-
Pseudomonas syringae
ir
D-glucuronate + NAD+ + H2O
Pseudomonas syringae
strong substrate specificity, best substrate
D-glucaric acid + NADH + H+
-
Pseudomonas syringae
ir
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas syringae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas syringae
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.2
-
-
Pseudomonas syringae
Storage Stability (protein specific)
Storage Stability
Organism
-10C, 50 mM Tris-HCl, pH 7.2, 2.9 mM NAD+, less then 10% loss of activity up to 6 months
Pseudomonas syringae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NAD+ + H2O
50% of the activity compared to D-glucoronate
286230
Pseudomonas syringae
D-galactarate + NADH + H+
-
286230
Pseudomonas syringae
ir
D-glucuronate + NAD+ + H2O
strong substrate specificity, best substrate
286230
Pseudomonas syringae
D-glucaric acid + NADH + H+
-
286230
Pseudomonas syringae
ir
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
stable
Pseudomonas syringae
30
35
loss of activity after 30 min
Pseudomonas syringae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8.4
-
Pseudomonas syringae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
50% remaining activity
Pseudomonas syringae
9.4
-
50% remaining activity
Pseudomonas syringae
Other publictions for EC 1.1.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725435
Parkkinen
Crystal structure of uronate d ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
J. Biol. Chem.
286
27294-27300
2011
-
-
1
1
1
-
-
-
-
-
-
4
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695552
Moon
Enzymatic assay of D-glucurona ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
Anal. Biochem.
392
183-185
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
20
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695783
Moon
Production of glucaric acid fr ...
Pseudomonas syringae pv. tomato
Appl. Environ. Microbiol.
75
589-595
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
698607
Yoon
Cloning and characterization o ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58, Pseudomonas putida KT2440, Pseudomonas syringae pv. tomato
J. Bacteriol.
191
1565-1573
2009
-
-
3
-
-
-
-
9
-
-
3
-
-
13
-
-
3
-
-
-
-
-
8
3
-
-
-
6
3
-
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
9
-
-
3
-
-
-
-
3
-
-
-
-
8
3
-
-
-
6
3
-
-
-
-
-
-
-
-
-
286231
Wagner
Uronic acid dehydrogenase from ...
Pseudomonas syringae
Eur. J. Biochem.
61
589-596
1976
-
-
-
-
-
1
4
3
-
-
2
2
-
2
-
-
1
-
1
1
1
2
3
1
1
-
3
2
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
3
-
-
2
2
-
-
-
1
1
1
1
2
3
1
1
-
3
2
-
-
-
-
-
-
-
-
-
-
286230
Bateman
Purification and properties of ...
Pseudomonas syringae
Arch. Biochem. Biophys.
136
97-105
1970
-
-
-
-
-
1
1
3
-
-
-
2
-
2
-
-
1
-
-
1
1
1
2
-
-
-
2
-
1
-
2
1
-
-
-
-
-
-
1
-
-
1
-
1
-
3
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-