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show all sequences of 1.1.1.202

Structural and biochemical characterisation of a NAD+-dependent alcohol dehydrogenase from Oenococcus oeni as a new model molecule for industrial biotechnology applications

Elleuche, S.; Fodor, K.; Klippel, B.; von der Heyde, A.; Wilmanns, M.; Antranikian, G.; Appl. Microbiol. Biotechnol. 97, 8963-8975 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Oenococcus oeni
Crystallization (Commentary)
Crystallization
Organism
to 3.2 A resolution. The electron density map around the active site shows no sign of a bound co-factor, but the active site metal could be located and identified as Ni2+
Oenococcus oeni
Inhibitors
Inhibitors
Commentary
Organism
Structure
imidazole
activity is reduced down to 32% in the presence of 1 mM imidazole and addition of 250 mM results in complete inactivity
Oenococcus oeni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NAD+
pH 9.0, 60C
Oenococcus oeni
0.2
-
NADH
pH 8.0, 60C
Oenococcus oeni
11.5
-
1,2-propanediol
pH 9.0, 60C
Oenococcus oeni
26.7
-
1,3-Propanediol
pH 9.0, 60C
Oenococcus oeni
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ni2+
located near active site, crystallization data. The activity with 1,3-propanediol is highly dependent on the presence of Ni2+
Oenococcus oeni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42200
-
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein
Oenococcus oeni
43000
-
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein
Oenococcus oeni
74000
-
gel filtration
Oenococcus oeni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oenococcus oeni
A0NIJ1
-
-
Oenococcus oeni ATCC BAA-1163
A0NIJ1
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1
-
pH 9.0, 60C
Oenococcus oeni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-propanediol + NAD+
-
721395
Oenococcus oeni
2-hydroxypropanal + NADH
-
-
-
?
1,2-propanediol + NAD+
-
721395
Oenococcus oeni ATCC BAA-1163
2-hydroxypropanal + NADH
-
-
-
?
1,3-propanediol + NAD+
activity with 1,3-propanediol is highly dependent on the presence of Ni2+
721395
Oenococcus oeni
3-hydroxypropanal + NADH
-
-
-
?
1,3-propanediol + NAD+
activity with 1,3-propanediol is highly dependent on the presence of Ni2+
721395
Oenococcus oeni ATCC BAA-1163
3-hydroxypropanal + NADH
-
-
-
?
additional information
enzyme additionally accepts ethanol, 1-propanol, 2-mercaptoethanol and their reduces their corresponding aldehydes. No substrates: methanol, 1-butanol, glycerol or 2-propanol
721395
Oenococcus oeni
?
-
-
-
-
additional information
enzyme additionally accepts ethanol, 1-propanol, 2-mercaptoethanol and their reduces their corresponding aldehydes. No substrates: methanol, 1-butanol, glycerol or 2-propanol
721395
Oenococcus oeni ATCC BAA-1163
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein
Oenococcus oeni
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
60
-
Oenococcus oeni
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
60 min, inactivation
Oenococcus oeni
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
reduction of aldehydes
Oenococcus oeni
9
-
oxidation of alcohols
Oenococcus oeni
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
7
most stable within this range
Oenococcus oeni
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADPH
Oenococcus oeni
NAD+
-
Oenococcus oeni
NADH
-
Oenococcus oeni
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Oenococcus oeni
calculated
-
5.2
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Oenococcus oeni
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADPH
Oenococcus oeni
NAD+
-
Oenococcus oeni
NADH
-
Oenococcus oeni
Crystallization (Commentary) (protein specific)
Crystallization
Organism
to 3.2 A resolution. The electron density map around the active site shows no sign of a bound co-factor, but the active site metal could be located and identified as Ni2+
Oenococcus oeni
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
imidazole
activity is reduced down to 32% in the presence of 1 mM imidazole and addition of 250 mM results in complete inactivity
Oenococcus oeni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NAD+
pH 9.0, 60C
Oenococcus oeni
0.2
-
NADH
pH 8.0, 60C
Oenococcus oeni
11.5
-
1,2-propanediol
pH 9.0, 60C
Oenococcus oeni
26.7
-
1,3-Propanediol
pH 9.0, 60C
Oenococcus oeni
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ni2+
located near active site, crystallization data. The activity with 1,3-propanediol is highly dependent on the presence of Ni2+
Oenococcus oeni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42200
-
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein
Oenococcus oeni
43000
-
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein
Oenococcus oeni
74000
-
gel filtration
Oenococcus oeni
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1
-
pH 9.0, 60C
Oenococcus oeni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-propanediol + NAD+
-
721395
Oenococcus oeni
2-hydroxypropanal + NADH
-
-
-
?
1,2-propanediol + NAD+
-
721395
Oenococcus oeni ATCC BAA-1163
2-hydroxypropanal + NADH
-
-
-
?
1,3-propanediol + NAD+
activity with 1,3-propanediol is highly dependent on the presence of Ni2+
721395
Oenococcus oeni
3-hydroxypropanal + NADH
-
-
-
?
1,3-propanediol + NAD+
activity with 1,3-propanediol is highly dependent on the presence of Ni2+
721395
Oenococcus oeni ATCC BAA-1163
3-hydroxypropanal + NADH
-
-
-
?
additional information
enzyme additionally accepts ethanol, 1-propanol, 2-mercaptoethanol and their reduces their corresponding aldehydes. No substrates: methanol, 1-butanol, glycerol or 2-propanol
721395
Oenococcus oeni
?
-
-
-
-
additional information
enzyme additionally accepts ethanol, 1-propanol, 2-mercaptoethanol and their reduces their corresponding aldehydes. No substrates: methanol, 1-butanol, glycerol or 2-propanol
721395
Oenococcus oeni ATCC BAA-1163
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein
Oenococcus oeni
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
60
-
Oenococcus oeni
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
60 min, inactivation
Oenococcus oeni
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
reduction of aldehydes
Oenococcus oeni
9
-
oxidation of alcohols
Oenococcus oeni
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
7
most stable within this range
Oenococcus oeni
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Oenococcus oeni
calculated
-
5.2
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.01
-
1,2-propanediol
pH 9.0, 60C
Oenococcus oeni
0.01
-
1,3-Propanediol
pH 9.0, 60C
Oenococcus oeni
4.9
-
NAD+
pH 9.0, 60C
Oenococcus oeni
12.9
-
NADH
pH 8.0, 60C
Oenococcus oeni
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.01
-
1,2-propanediol
pH 9.0, 60C
Oenococcus oeni
0.01
-
1,3-Propanediol
pH 9.0, 60C
Oenococcus oeni
4.9
-
NAD+
pH 9.0, 60C
Oenococcus oeni
12.9
-
NADH
pH 8.0, 60C
Oenococcus oeni
Other publictions for EC 1.1.1.202
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721395
Elleuche
Structural and biochemical cha ...
Oenococcus oeni, Oenococcus oeni ATCC BAA-1163
Appl. Microbiol. Biotechnol.
97
8963-8975
2013
-
-
1
1
-
-
1
4
-
1
3
-
-
5
-
-
-
-
-
-
1
-
6
1
1
-
1
-
2
-
1
3
-
1
-
-
-
1
3
1
-
-
-
1
-
4
-
1
3
-
-
-
-
-
-
-
1
-
6
1
1
-
1
-
2
-
1
1
-
-
-
-
4
4
724566
Oh
Efficient production of 1,3-pr ...
Klebsiella pneumoniae
Bioprocess Biosyst. Eng.
36
757-763
2013
-
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
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1
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2
-
-
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-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725951
Qi
Molecular cloning, co-expressi ...
Citrobacter freundii
Mol. Biotechnol.
54
469-474
2013
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
-
-
-
-
2
-
2
1
2
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2
2
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2
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-
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
2
-
2
1
2
-
-
-
2
2
-
-
-
-
-
-
-
-
725880
Stevens
1,3-Propanediol dehydrogenases ...
Lactobacillus reuteri
Microb. Cell Fact.
10
61
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
-
-
-
-
-
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-
1
1
-
-
-
695716
Ma
Construction of a Novel Expres ...
Klebsiella pneumoniae
Appl. Biochem. Biotechnol.
162
399-407
2010
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710747
Jeyakanthan
Expression, purification and X ...
Aquifex aeolicus
Acta Crystallogr. Sect. F
66
184-186
2010
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710972
Zhuge
Expression of 1,3-propanediol ...
Escherichia coli, Klebsiella pneumoniae
Appl. Microbiol. Biotechnol.
87
2177-2184
2010
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
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-
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712531
Ma
Relaxing the coenzyme specific ...
Klebsiella pneumoniae
J. Biotechnol.
146
173-178
2010
-
-
-
1
2
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
6
-
-
-
2
-
-
-
-
-
-
2
1
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
695764
Tang
Microbial conversion of glycer ...
Escherichia coli, Escherichia coli SYU
Appl. Environ. Microbiol.
75
1628-1634
2009
1
1
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
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2
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1
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1
1
1
1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695842
Seo
Elimination of by-product form ...
Klebsiella pneumoniae, Klebsiella pneumoniae Cu
Appl. Microbiol. Biotechnol.
84
527-534
2009
1
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
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1
-
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1
-
1
1
-
1
-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695844
Seo
Identification and utilization ...
Escherichia coli, Klebsiella pneumoniae
Appl. Microbiol. Biotechnol.
85
659-666
2009
-
-
1
-
1
-
-
-
-
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4
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-
-
-
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1
-
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1
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1
1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696785
Zhao
Effects of over-expression of ...
Klebsiella pneumoniae
Bioprocess Biosyst. Eng.
32
313-320
2009
-
-
1
-
-
-
-
-
-
-
-
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2
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-
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-
-
-
2
-
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-
-
-
-
-
-
1
-
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-
1
1
-
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-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697666
Chen
-
Regulation of 3-hydroxypropion ...
Klebsiella pneumoniae
Enzyme Microb. Technol.
45
305-309
2009
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
2
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698602
Marcal
1,3-Propanediol dehydrogenase ...
Klebsiella pneumoniae
J. Bacteriol.
191
1143-1151
2009
-
-
1
1
-
-
1
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
1
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699149
Zhao
-
Over-expression of glycerol de ...
Klebsiella pneumoniae
J. Chem. Technol. Biotechnol.
84
626-632
2009
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
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684524
Nemeth
Development of a new bioproces ...
Klebsiella pneumoniae
Appl. Biochem. Biotechnol.
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2008
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Decrease of 3-hydroxypropional ...
Klebsiella pneumoniae, Klebsiella pneumoniae TUAC01
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35
735-741
2008
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Hao
3-Hydroxypropionaldehyde guide ...
Klebsiella pneumoniae
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35
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2008
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675901
Wang
Production of 1,3-propanediol ...
Klebsiella pneumoniae
Mol. Biotechnol.
37
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2007
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684171
Marcal
Crystallization and preliminar ...
Klebsiella pneumoniae
Acta Crystallogr. Sect. F
63
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2007
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667270
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Clostridium butyricum, Clostridium butyricum VPI 3266
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2006
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664422
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Enhancement of 1,3-propanediol ...
Klebsiella pneumoniae, Klebsiella pneumoniae M5a1
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2005
1
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Fenghuan
High-level expression of the 1 ...
Klebsiella pneumoniae
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2005
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655107
Zheng
Cloning and sequence analysis ...
Klebsiella pneumoniae
Biotechnol. Lett.
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657320
Schwarzenbacher
Crystal structure of an iron-c ...
Thermotoga maritima
Proteins
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2003
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657406
Nemeth
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1,3-Propanediol oxidoreductase ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
World J. Microbiol. Biotechnol.
19
659-663
2003
1
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389496
Malaoui
Purification and characterizat ...
Clostridium butyricum, Clostridium butyricum E5
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2000
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Abbad-Andaloussi
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Clostridium butyricum
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1998
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Luers
Glycerol conversion to 1,3-pro ...
Clostridium pasteurianum
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154
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1997
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389498
Veiga-da-Cunha
1,3-Propanediol:NAD+ oxidoredu ...
Lactobacillus brevis, Lactobacillus buchneri
Appl. Environ. Microbiol.
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1992
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389489
Heyndrickx
-
The fermentation of glycerol b ...
Clostridium butyricum, Clostridium butyricum LMG 1212t2, Clostridium pasteurianum, Clostridium pasteurianum LMG 3285
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Talarico
Utilization of glycerol as a h ...
Lactobacillus reuteri
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56
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Sprenger
Anaerobic growth of Escherichi ...
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Klebsiella pneumoniae 1,3-prop ...
Klebsiella pneumoniae
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Abeles
beta-Hydroxypropionaldehyde, a ...
Klebsiella pneumoniae
Biochim. Biophys. Acta
41
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1960
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