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Literature summary for 1.1.1.202 extracted from

  • Fenghuan, W.; Huijin, Q.; He, H.; Tan, T.
    High-level expression of the 1,3-propanediol oxidoreductase from Klebsiella pneumoniae in Escherichia coli (2005), Mol. Biotechnol., 31, 211-219.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene dhaT, cloning from genomic DNA, DNA sequence determination, inducible high-level expression of the mostly soluble enzyme in Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
NAD+ pH 9.5, 30°C, recombinant enzyme Klebsiella pneumoniae
8.5
-
Propane-1,3-diol pH 9.5, 30°C, recombinant enzyme Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, recombinant enzyme, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropionaldehyde + NADH + H+ Klebsiella pneumoniae the enzyme is important in vivo for convertion of glycerol into propane-1,3-diol as second step after dehydration of glycerol by coenzyme B12-dependent glycerol dehydratase propane-1,3-diol + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
gene dhaT
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropionaldehyde + NADH + H+
-
Klebsiella pneumoniae propane-1,3-diol + NAD+
-
r
3-hydroxypropionaldehyde + NADH + H+ the enzyme is important in vivo for convertion of glycerol into propane-1,3-diol as second step after dehydration of glycerol by coenzyme B12-dependent glycerol dehydratase Klebsiella pneumoniae propane-1,3-diol + NAD+
-
r

Subunits

Subunits Comment Organism
? x * 42000, recombinant enzyme, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
1,3-propanediol oxidoreductase
-
Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant enzyme Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
recombinant enzyme Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Klebsiella pneumoniae
NADH
-
Klebsiella pneumoniae