BRENDA - Enzyme Database show
show all sequences of 1.1.1.201

Identification, cloning, heterologous expression, and characterization of a NADPH-dependent 7beta-hydroxysteroid dehydrogenase from Collinsella aerofaciens

Liu, L.; Aigner, A.; Schmid, R.D.; Appl. Microbiol. Biotechnol. 90, 127-135 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Collinsella aerofaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0045
-
NADPH
pH 8.0, 25C
Collinsella aerofaciens
0.0052
-
7-ketolithocholic acid
pH 8.0, 25C
Collinsella aerofaciens
0.0053
-
NADP+
pH 8.0, 25C
Collinsella aerofaciens
0.0062
-
Ursodeoxycholic acid
pH 8.0, 25C
Collinsella aerofaciens
0.0092
-
Dehydrocholic acid
pH 8.0, 25C
Collinsella aerofaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Collinsella aerofaciens
56100
-
gel filtration
Collinsella aerofaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Collinsella aerofaciens
-
-
-
Collinsella aerofaciens DSM 3979
-
-
-
Storage Stability
Storage Stability
Organism
-20C, 50 mM potassium phosphate, pH 8.0, stable for at least 3 months with several times of freezing and thawing
Collinsella aerofaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-ketolithocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens
ursodeoxycholic acid + NADP+
only product, 90% conversion, 71% yield
-
-
r
7-ketolithocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens DSM 3979
ursodeoxycholic acid + NADP+
only product, 90% conversion, 71% yield
-
-
r
dehydrocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens
? + NADP+ + H+
-
-
-
?
dehydrocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens DSM 3979
? + NADP+ + H+
-
-
-
?
ursodeoxycholic acid + NADP+
-
724054
Collinsella aerofaciens
7-ketolithocholic acid + NADPH + H+
-
-
-
r
ursodeoxycholic acid + NADP+
-
724054
Collinsella aerofaciens DSM 3979
7-ketolithocholic acid + NADPH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Collinsella aerofaciens
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
complete inactivation within 400 min
Collinsella aerofaciens
50
-
complete inactivation within 5 min
Collinsella aerofaciens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14.6
-
Dehydrocholic acid
pH 8.0, 25C
Collinsella aerofaciens
15.7
-
NADP+
pH 8.0, 25C
Collinsella aerofaciens
15.8
-
7-ketolithocholic acid
pH 8.0, 25C
Collinsella aerofaciens
17.2
-
NADPH
pH 8.0, 25C
Collinsella aerofaciens
19.6
-
Ursodeoxycholic acid
pH 8.0, 25C
Collinsella aerofaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Collinsella aerofaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0045
-
NADPH
pH 8.0, 25C
Collinsella aerofaciens
0.0052
-
7-ketolithocholic acid
pH 8.0, 25C
Collinsella aerofaciens
0.0053
-
NADP+
pH 8.0, 25C
Collinsella aerofaciens
0.0062
-
Ursodeoxycholic acid
pH 8.0, 25C
Collinsella aerofaciens
0.0092
-
Dehydrocholic acid
pH 8.0, 25C
Collinsella aerofaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Collinsella aerofaciens
56100
-
gel filtration
Collinsella aerofaciens
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 50 mM potassium phosphate, pH 8.0, stable for at least 3 months with several times of freezing and thawing
Collinsella aerofaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-ketolithocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens
ursodeoxycholic acid + NADP+
only product, 90% conversion, 71% yield
-
-
r
7-ketolithocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens DSM 3979
ursodeoxycholic acid + NADP+
only product, 90% conversion, 71% yield
-
-
r
dehydrocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens
? + NADP+ + H+
-
-
-
?
dehydrocholic acid + NADPH + H+
-
724054
Collinsella aerofaciens DSM 3979
? + NADP+ + H+
-
-
-
?
ursodeoxycholic acid + NADP+
-
724054
Collinsella aerofaciens
7-ketolithocholic acid + NADPH + H+
-
-
-
r
ursodeoxycholic acid + NADP+
-
724054
Collinsella aerofaciens DSM 3979
7-ketolithocholic acid + NADPH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Collinsella aerofaciens
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
complete inactivation within 400 min
Collinsella aerofaciens
50
-
complete inactivation within 5 min
Collinsella aerofaciens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14.6
-
Dehydrocholic acid
pH 8.0, 25C
Collinsella aerofaciens
15.7
-
NADP+
pH 8.0, 25C
Collinsella aerofaciens
15.8
-
7-ketolithocholic acid
pH 8.0, 25C
Collinsella aerofaciens
17.2
-
NADPH
pH 8.0, 25C
Collinsella aerofaciens
19.6
-
Ursodeoxycholic acid
pH 8.0, 25C
Collinsella aerofaciens
Other publictions for EC 1.1.1.201
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724054
Liu
Identification, cloning, heter ...
Collinsella aerofaciens, Collinsella aerofaciens DSM 3979
Appl. Microbiol. Biotechnol.
90
127-135
2011
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1
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5
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2
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2
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1
6
1
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2
5
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1
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5
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2
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1
6
1
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2
5
-
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-
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-
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656659
Shiotsuki
Purification and characterizat ...
Oryctolagus cuniculus
J. Steroid Biochem. Mol. Biol.
91
185-190
2004
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-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
2
1
-
5
1
1
-
-
-
2
-
-
1
-
-
-
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-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
2
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
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286228
Edenharder
Characterization of NAD-depend ...
Blautia producta
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
-
7
2
-
1
11
-
1
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
7
2
-
1
11
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286229
Edenharder
NADP-dependent 3beta-, 7alpha- ...
Clostridium sp. 25.11.c, Clostridium sp.
Biochim. Biophys. Acta
1002
37-44
1989
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-
-
-
-
1
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286227
Sutherland
Bile acid induction of 7alpha- ...
Hathewaya limosa
J. Lipid Res.
26
344-350
1985
-
-
-
-
-
-
-
3
-
-
-
3
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286224
Macdonald
Formation of ursodeoxycholic a ...
Collinsella aerofaciens
Appl. Environ. Microbiol.
44
1187-1195
1982
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286226
Hirano
Characterization of NADP-depen ...
Blautia producta, Collinsella aerofaciens
Appl. Environ. Microbiol.
43
1057-1063
1982
-
-
-
-
-
-
2
8
-
-
2
8
-
2
-
-
2
-
-
2
3
-
12
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
8
-
-
2
8
-
-
-
2
-
2
3
-
12
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
286076
MacDonald
Bile induction of 7alpha- and ...
Clostridium sardiniense
Biochim. Biophys. Acta
665
262-269
1981
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
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-
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2
-
-
-
1
-
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-
1
-
-
2
-
-
-
-
-
1
-
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-
-
-
-
-
-
-