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show all sequences of 1.1.1.201

Purification and characterization of 7beta-hydroxysteroid dehydrogenase from rabbit liver microsomes

Shiotsuki, H.; Maeda, Y.; Chijiiwa, K.; J. Steroid Biochem. Mol. Biol. 91, 185-190 (2004)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
x * 34000, SDS-PAGE
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7beta-hydroxycholesterol + NADP+
Oryctolagus cuniculus
-
7-oxocholesterol + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme from liver microsomes 300fold by ion exchange, hydroxylapatite, 2',5'-ADP affinity
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.276
-
purified enzyme
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-cholesten-3beta,7beta-diol + NADP+
i.e. 7beta-hydroxycholesterol
656659
Oryctolagus cuniculus
5-cholesten-3beta-ol-7-one + NADPH
-
-
-
?
7beta-hydroxycholesterol + NADP+
-
656659
Oryctolagus cuniculus
7-oxocholesterol + NADPH
-
-
-
?
corticosterone + NADP+
oxidation at C-11
656659
Oryctolagus cuniculus
21-hydroxy-pregn-4-ene-3,11,20-trione + NADPH
-
-
-
?
cortisone + NADP+
oxidation at C-11
656659
Oryctolagus cuniculus
? + NADPH
-
-
-
?
additional information
enzyme might also show 11beta-hydroxysteroid dehydrogenase activity, no activity with cholesterol, 7alpha-hydroxycholesterol, 25-hydroxycholesterol, pregnenolone, dehydroepiandrosterone, chenodoxycolic acid, and ursodeoxycholic acid
656659
Oryctolagus cuniculus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 34000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
potassium buffer
Oryctolagus cuniculus
11
-
glycine-NaOH buffer
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
beta-form is preferred
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
beta-form is preferred
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
x * 34000, SDS-PAGE
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7beta-hydroxycholesterol + NADP+
Oryctolagus cuniculus
-
7-oxocholesterol + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme from liver microsomes 300fold by ion exchange, hydroxylapatite, 2',5'-ADP affinity
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.276
-
purified enzyme
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-cholesten-3beta,7beta-diol + NADP+
i.e. 7beta-hydroxycholesterol
656659
Oryctolagus cuniculus
5-cholesten-3beta-ol-7-one + NADPH
-
-
-
?
7beta-hydroxycholesterol + NADP+
-
656659
Oryctolagus cuniculus
7-oxocholesterol + NADPH
-
-
-
?
corticosterone + NADP+
oxidation at C-11
656659
Oryctolagus cuniculus
21-hydroxy-pregn-4-ene-3,11,20-trione + NADPH
-
-
-
?
cortisone + NADP+
oxidation at C-11
656659
Oryctolagus cuniculus
? + NADPH
-
-
-
?
additional information
enzyme might also show 11beta-hydroxysteroid dehydrogenase activity, no activity with cholesterol, 7alpha-hydroxycholesterol, 25-hydroxycholesterol, pregnenolone, dehydroepiandrosterone, chenodoxycolic acid, and ursodeoxycholic acid
656659
Oryctolagus cuniculus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
potassium buffer
Oryctolagus cuniculus
11
-
glycine-NaOH buffer
Oryctolagus cuniculus
Other publictions for EC 1.1.1.201
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724054
Liu
Identification, cloning, heter ...
Collinsella aerofaciens, Collinsella aerofaciens DSM 3979
Appl. Microbiol. Biotechnol.
90
127-135
2011
-
-
1
-
-
-
-
5
-
-
2
-
-
2
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
-
656659
Shiotsuki
Purification and characterizat ...
Oryctolagus cuniculus
J. Steroid Biochem. Mol. Biol.
91
185-190
2004
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
2
1
-
5
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
2
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
-
7
2
-
1
11
-
1
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
7
2
-
1
11
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286229
Edenharder
NADP-dependent 3beta-, 7alpha- ...
Clostridium sp. 25.11.c, Clostridium sp.
Biochim. Biophys. Acta
1002
37-44
1989
-
-
-
-
-
1
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286227
Sutherland
Bile acid induction of 7alpha- ...
Hathewaya limosa
J. Lipid Res.
26
344-350
1985
-
-
-
-
-
-
-
3
-
-
-
3
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286224
Macdonald
Formation of ursodeoxycholic a ...
Collinsella aerofaciens
Appl. Environ. Microbiol.
44
1187-1195
1982
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286226
Hirano
Characterization of NADP-depen ...
Blautia producta, Collinsella aerofaciens
Appl. Environ. Microbiol.
43
1057-1063
1982
-
-
-
-
-
-
2
8
-
-
2
8
-
2
-
-
2
-
-
2
3
-
12
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
8
-
-
2
8
-
-
-
2
-
2
3
-
12
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
286076
MacDonald
Bile induction of 7alpha- and ...
Clostridium sardiniense
Biochim. Biophys. Acta
665
262-269
1981
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-