BRENDA - Enzyme Database show
show all sequences of 1.1.1.201

NADP-dependent 3beta-, 7alpha- and 7beta-hydroxysteroid dehydrogenase activities from a lecithinase-lipase-negative Clostridium species 25.11.c

Edenharder, R.; Pfutzner, A.; Hammann, R.; Biochim. Biophys. Acta 1002, 37-44 (1989)

Data extracted from this reference:

General Stability
General Stability
Organism
heat-sensitive
Clostridium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
115000
-
gel filtration
Clostridium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Clostridium sp.
activity only in the reductive reaction
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Clostridium sp. 25.11.c
activity only in the reductive reaction
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium sp.
-
strain 25.11.c
-
Clostridium sp. 25.11.c
-
strain 25.11.c
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Clostridium sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp.
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp. 25.11.c
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp.
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp. 25.11.c
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
-
ir
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
-
Clostridium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Clostridium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Clostridium sp.
General Stability (protein specific)
General Stability
Organism
heat-sensitive
Clostridium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
115000
-
gel filtration
Clostridium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Clostridium sp.
activity only in the reductive reaction
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Clostridium sp. 25.11.c
activity only in the reductive reaction
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Clostridium sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp.
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp. 25.11.c
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp.
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
activity only in the reductive reaction
286229
Clostridium sp. 25.11.c
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
-
-
ir
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
-
Clostridium sp.
Other publictions for EC 1.1.1.201
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724054
Liu
Identification, cloning, heter ...
Collinsella aerofaciens, Collinsella aerofaciens DSM 3979
Appl. Microbiol. Biotechnol.
90
127-135
2011
-
-
1
-
-
-
-
5
-
-
2
-
-
2
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
-
656659
Shiotsuki
Purification and characterizat ...
Oryctolagus cuniculus
J. Steroid Biochem. Mol. Biol.
91
185-190
2004
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
2
1
-
5
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
2
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
-
7
2
-
1
11
-
1
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
7
2
-
1
11
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286229
Edenharder
NADP-dependent 3beta-, 7alpha- ...
Clostridium sp. 25.11.c, Clostridium sp.
Biochim. Biophys. Acta
1002
37-44
1989
-
-
-
-
-
1
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286227
Sutherland
Bile acid induction of 7alpha- ...
Hathewaya limosa
J. Lipid Res.
26
344-350
1985
-
-
-
-
-
-
-
3
-
-
-
3
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286224
Macdonald
Formation of ursodeoxycholic a ...
Collinsella aerofaciens
Appl. Environ. Microbiol.
44
1187-1195
1982
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286226
Hirano
Characterization of NADP-depen ...
Blautia producta, Collinsella aerofaciens
Appl. Environ. Microbiol.
43
1057-1063
1982
-
-
-
-
-
-
2
8
-
-
2
8
-
2
-
-
2
-
-
2
3
-
12
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
8
-
-
2
8
-
-
-
2
-
2
3
-
12
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
286076
MacDonald
Bile induction of 7alpha- and ...
Clostridium sardiniense
Biochim. Biophys. Acta
665
262-269
1981
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-