BRENDA - Enzyme Database show
show all sequences of 1.1.1.201

Bile acid induction of 7alpha- and 7beta-hydroxysteroid dehydrogenases in Clostridium limosum

Sutherland, J.D.; Williams, C.N.; J. Lipid Res. 26, 344-350 (1985)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Hathewaya limosa
0.076
-
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid
-
Hathewaya limosa
0.14
-
NADP+
-
Hathewaya limosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
Hathewaya limosa
ursocholic acid
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Hathewaya limosa
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Hathewaya limosa
active on both free and conjugated bile salts
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Hathewaya limosa
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hathewaya limosa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
bile salt induction of enzymes synthesis
Hathewaya limosa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
ursocholic acid
286227
Hathewaya limosa
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286227
Hathewaya limosa
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
active on both free and conjugated bile salts
286227
Hathewaya limosa
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286227
Hathewaya limosa
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
active on both free and conjugated bile salts
286227
Hathewaya limosa
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.8
10.5
-
Hathewaya limosa
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Hathewaya limosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Hathewaya limosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Hathewaya limosa
0.076
-
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid
-
Hathewaya limosa
0.14
-
NADP+
-
Hathewaya limosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
Hathewaya limosa
ursocholic acid
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Hathewaya limosa
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Hathewaya limosa
active on both free and conjugated bile salts
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Hathewaya limosa
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
bile salt induction of enzymes synthesis
Hathewaya limosa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
ursocholic acid
286227
Hathewaya limosa
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286227
Hathewaya limosa
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
active on both free and conjugated bile salts
286227
Hathewaya limosa
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286227
Hathewaya limosa
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
active on both free and conjugated bile salts
286227
Hathewaya limosa
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286227
Hathewaya limosa
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.8
10.5
-
Hathewaya limosa
Other publictions for EC 1.1.1.201
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724054
Liu
Identification, cloning, heter ...
Collinsella aerofaciens, Collinsella aerofaciens DSM 3979
Appl. Microbiol. Biotechnol.
90
127-135
2011
-
-
1
-
-
-
-
5
-
-
2
-
-
2
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
-
656659
Shiotsuki
Purification and characterizat ...
Oryctolagus cuniculus
J. Steroid Biochem. Mol. Biol.
91
185-190
2004
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
2
1
-
5
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
2
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
-
7
2
-
1
11
-
1
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
7
2
-
1
11
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286229
Edenharder
NADP-dependent 3beta-, 7alpha- ...
Clostridium sp. 25.11.c, Clostridium sp.
Biochim. Biophys. Acta
1002
37-44
1989
-
-
-
-
-
1
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286227
Sutherland
Bile acid induction of 7alpha- ...
Hathewaya limosa
J. Lipid Res.
26
344-350
1985
-
-
-
-
-
-
-
3
-
-
-
3
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286224
Macdonald
Formation of ursodeoxycholic a ...
Collinsella aerofaciens
Appl. Environ. Microbiol.
44
1187-1195
1982
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286226
Hirano
Characterization of NADP-depen ...
Blautia producta, Collinsella aerofaciens
Appl. Environ. Microbiol.
43
1057-1063
1982
-
-
-
-
-
-
2
8
-
-
2
8
-
2
-
-
2
-
-
2
3
-
12
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
8
-
-
2
8
-
-
-
2
-
2
3
-
12
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
286076
MacDonald
Bile induction of 7alpha- and ...
Clostridium sardiniense
Biochim. Biophys. Acta
665
262-269
1981
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-