BRENDA - Enzyme Database show
show all sequences of 1.1.1.201

Characterization of NADP-dependent 7beta-hydroxysteroid dehydrogenases from Peptostreptococcus productus and Eubacterium aerofaciens

Hirano, S.; Masuda, N.; Appl. Environ. Microbiol. 43, 1057-1063 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetate
complete inhibition at 0.05 mM
Blautia producta
p-chloromercuribenzoic acid
complete inhibition at 0.001 mM
Blautia producta
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Blautia producta
0.1
-
NADP+
-
Blautia producta
0.108
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Collinsella aerofaciens
0.238
-
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
Blautia producta
0.286
-
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
Blautia producta
0.4
-
NADP+
-
Collinsella aerofaciens
0.909
-
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
Collinsella aerofaciens
1.639
-
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
Collinsella aerofaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Collinsella aerofaciens
53000
-
gel filtration
Blautia producta
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Blautia producta
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
only active on free and conjugated bile salts
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Blautia producta
only active on free and conjugated bile salts
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
Collinsella aerofaciens
-
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
Blautia producta
-
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
Collinsella aerofaciens
-
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
Blautia producta
-
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
Blautia producta
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Blautia producta
-
-
-
Collinsella aerofaciens
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Blautia producta
to homogeneity, chromatography techniques
Collinsella aerofaciens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Collinsella aerofaciens
-
cell culture
-
Blautia producta
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.1
-
crude cell extract
Collinsella aerofaciens
0.17
-
-
Collinsella aerofaciens
1.06
-
-
Blautia producta
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Blautia producta
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Blautia producta
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Blautia producta
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Blautia producta
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
286226
Collinsella aerofaciens
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
286226
Blautia producta
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
286226
Collinsella aerofaciens
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
286226
Blautia producta
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
286226
Blautia producta
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
50% loss of activity within 30 min
Blautia producta
50
-
90% loss of activity within 3 min
Blautia producta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
11
-
Collinsella aerofaciens
9.5
10.5
-
Blautia producta
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Collinsella aerofaciens
NADP+
no activity with NAD+
Blautia producta
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Collinsella aerofaciens
NADP+
no activity with NAD+
Blautia producta
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetate
complete inhibition at 0.05 mM
Blautia producta
p-chloromercuribenzoic acid
complete inhibition at 0.001 mM
Blautia producta
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Blautia producta
0.1
-
NADP+
-
Blautia producta
0.108
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Collinsella aerofaciens
0.238
-
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
Blautia producta
0.286
-
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
Blautia producta
0.4
-
NADP+
-
Collinsella aerofaciens
0.909
-
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
Collinsella aerofaciens
1.639
-
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
Collinsella aerofaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Collinsella aerofaciens
53000
-
gel filtration
Blautia producta
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Blautia producta
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
only active on free and conjugated bile salts
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Blautia producta
only active on free and conjugated bile salts
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
Collinsella aerofaciens
-
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
Blautia producta
-
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
Collinsella aerofaciens
-
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
Blautia producta
-
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
Blautia producta
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Blautia producta
to homogeneity, chromatography techniques
Collinsella aerofaciens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Collinsella aerofaciens
-
cell culture
-
Blautia producta
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.1
-
crude cell extract
Collinsella aerofaciens
0.17
-
-
Collinsella aerofaciens
1.06
-
-
Blautia producta
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Blautia producta
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Blautia producta
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286226
Blautia producta
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
only active on free and conjugated bile salts
286226
Blautia producta
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
286226
Collinsella aerofaciens
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
286226
Blautia producta
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
286226
Blautia producta
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
286226
Collinsella aerofaciens
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
286226
Collinsella aerofaciens
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
286226
Blautia producta
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
286226
Blautia producta
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
50% loss of activity within 30 min
Blautia producta
50
-
90% loss of activity within 3 min
Blautia producta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
11
-
Collinsella aerofaciens
9.5
10.5
-
Blautia producta
Other publictions for EC 1.1.1.201
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724054
Liu
Identification, cloning, heter ...
Collinsella aerofaciens, Collinsella aerofaciens DSM 3979
Appl. Microbiol. Biotechnol.
90
127-135
2011
-
-
1
-
-
-
-
5
-
-
2
-
-
2
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
-
656659
Shiotsuki
Purification and characterizat ...
Oryctolagus cuniculus
J. Steroid Biochem. Mol. Biol.
91
185-190
2004
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
2
1
-
5
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
2
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
-
7
2
-
1
11
-
1
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
7
2
-
1
11
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286229
Edenharder
NADP-dependent 3beta-, 7alpha- ...
Clostridium sp. 25.11.c, Clostridium sp.
Biochim. Biophys. Acta
1002
37-44
1989
-
-
-
-
-
1
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286227
Sutherland
Bile acid induction of 7alpha- ...
Hathewaya limosa
J. Lipid Res.
26
344-350
1985
-
-
-
-
-
-
-
3
-
-
-
3
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286224
Macdonald
Formation of ursodeoxycholic a ...
Collinsella aerofaciens
Appl. Environ. Microbiol.
44
1187-1195
1982
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286226
Hirano
Characterization of NADP-depen ...
Blautia producta, Collinsella aerofaciens
Appl. Environ. Microbiol.
43
1057-1063
1982
-
-
-
-
-
-
2
8
-
-
2
8
-
2
-
-
2
-
-
2
3
-
12
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
8
-
-
2
8
-
-
-
2
-
2
3
-
12
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
286076
MacDonald
Bile induction of 7alpha- and ...
Clostridium sardiniense
Biochim. Biophys. Acta
665
262-269
1981
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-