BRENDA - Enzyme Database show
show all sequences of 1.1.1.201

Formation of ursodeoxycholic acid from chenodeoxycholic acid by a 7beta-hydroxysteroid dehydrogenase-elaborating Eubacterium aerofaciens strain cocultured with 7alpha-hydroxysteroid dehydrogenase-elaborating organisms

Macdonald, I.A.; Rochon, Y.P.; Hutchinson, D.M.; Holdeman, L.V.; Appl. Environ. Microbiol. 44, 1187-1195 (1982)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
partially reversible, poor substrate
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
ursocholic acid
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
partially reversible
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
Collinsella aerofaciens
partially reversible, poore substrate
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
Collinsella aerofaciens
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Collinsella aerofaciens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Collinsella aerofaciens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
partially reversible, poor substrate
286224
Collinsella aerofaciens
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
ursocholic acid
286224
Collinsella aerofaciens
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
partially reversible
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
partially reversible
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
partially reversible, poore substrate
286224
Collinsella aerofaciens
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
286224
Collinsella aerofaciens
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.8
10.3
-
Collinsella aerofaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Collinsella aerofaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Collinsella aerofaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
partially reversible, poor substrate
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
ursocholic acid
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
ursodeoxycholic acid
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
Collinsella aerofaciens
partially reversible
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
Collinsella aerofaciens
partially reversible, poore substrate
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
Collinsella aerofaciens
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Collinsella aerofaciens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
partially reversible, poor substrate
286224
Collinsella aerofaciens
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
ursocholic acid
286224
Collinsella aerofaciens
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
partially reversible
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
ursodeoxycholic acid
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
partially reversible
286224
Collinsella aerofaciens
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
-
286224
Collinsella aerofaciens
r
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
partially reversible, poore substrate
286224
Collinsella aerofaciens
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
286224
Collinsella aerofaciens
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.8
10.3
-
Collinsella aerofaciens
Other publictions for EC 1.1.1.201
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724054
Liu
Identification, cloning, heter ...
Collinsella aerofaciens, Collinsella aerofaciens DSM 3979
Appl. Microbiol. Biotechnol.
90
127-135
2011
-
-
1
-
-
-
-
5
-
-
2
-
-
2
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
6
1
-
-
2
5
-
-
-
-
-
-
-
-
-
-
656659
Shiotsuki
Purification and characterizat ...
Oryctolagus cuniculus
J. Steroid Biochem. Mol. Biol.
91
185-190
2004
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
2
1
-
5
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
2
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
-
7
2
-
1
11
-
1
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
7
2
-
1
11
-
-
-
-
-
1
-
-
13
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286229
Edenharder
NADP-dependent 3beta-, 7alpha- ...
Clostridium sp. 25.11.c, Clostridium sp.
Biochim. Biophys. Acta
1002
37-44
1989
-
-
-
-
-
1
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286227
Sutherland
Bile acid induction of 7alpha- ...
Hathewaya limosa
J. Lipid Res.
26
344-350
1985
-
-
-
-
-
-
-
3
-
-
-
3
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286224
Macdonald
Formation of ursodeoxycholic a ...
Collinsella aerofaciens
Appl. Environ. Microbiol.
44
1187-1195
1982
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286226
Hirano
Characterization of NADP-depen ...
Blautia producta, Collinsella aerofaciens
Appl. Environ. Microbiol.
43
1057-1063
1982
-
-
-
-
-
-
2
8
-
-
2
8
-
2
-
-
2
-
-
2
3
-
12
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
8
-
-
2
8
-
-
-
2
-
2
3
-
12
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
286076
MacDonald
Bile induction of 7alpha- and ...
Clostridium sardiniense
Biochim. Biophys. Acta
665
262-269
1981
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-