Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.21 | - |
D-sorbitol 6-phosphate | at pH 8.0 and 25°C | Oryza sativa | |
15.9 | - |
D-glucose 6-phosphate | at pH 8.0 and 25°C | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.477 | - |
with D-sorbitol 6-phosphate as substrate, at pH 8.0 and 25°C | Oryza sativa |
0.603 | - |
with D-glucose 6-phosphate as substrate, at pH 8.0 and 25°C | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADPH + H+ | - |
Oryza sativa | D-sorbitol 6-phosphate + NADP+ | - |
r | |
D-sorbitol 6-phosphate + NADP+ | - |
Oryza sativa | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
additional information | no activity against mannose 6-phosphate | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
S6PDH | - |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Oryza sativa | |
NADPH | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
physiological function | sorbitol-6-phosphate dehydrogenase is a key enzyme for sorbitol synthesis and plays an important role in the alleviation of salinity stress in plants | Oryza sativa |