BRENDA - Enzyme Database show
show all sequences of 1.1.1.200

Aldose-6-phosphate reductase from apple leaves: Importance of the quaternary structure for enzyme activity

Figueroa, C.M.; Iglesias, A.A.; Biochimie 92, 81-88 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
amplified gene cloned into the pGEM-T Easy vector, heterologous expression with a His-tag alternatively placed in the N- or C-terminus in Escherichia coli BL21 Star (DE3) cells
Malus domestica
Inhibitors
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
-
Malus domestica
KCl
-
Malus domestica
additional information
no significant differences in activity when ATP, ADP, AMP, ADP-glucose, UTP, UDP-glucose, inorganic phosphate or diphosphate (5 mM each), 10% (v/v) glycerol, 500 mM glucitol or 20% (w/v) sucrose are added to the enzyme assay mixture
Malus domestica
NaCl
200 mM cause about 65% inhibition of the enzyme activity
Malus domestica
NH4Cl
-
Malus domestica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
level of soluble recombinant enzyme is much higher in the N-term tagged protein than in the C-term tagged one. In the C-term tagged one the majority of the protein is in the insoluble form
Malus domestica
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34900
-
sequence analysis, A6PRase
Malus domestica
39000
-
gel filtration, C-term tagged enzyme
Malus domestica
68000
-
gel filtration, N-term tagged enzyme
Malus domestica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Malus domestica
P28475
-
-
Purification (Commentary)
Commentary
Organism
one-step protein purification by immobilized metal affinity chromatography, N-term tagged protein 95% pure, C-term tagged one 75% pure
Malus domestica
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Malus domestica
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.017
-
C-term tagged enzyme, purified enzyme
Malus domestica
0.136
-
N-term tagged enzyme, crude extract
Malus domestica
1.25
-
N-term tagged enzyme, 9.2fold purified
Malus domestica
Storage Stability
Storage Stability
Organism
-80░C, 25 mM Tris-HCl buffer, pH 8.0, 300 mM NaCl, 1 mM beta-mercaptoethanol, 10% (v/v) glycerol, 1 mM EDTA, 0.5 mM DTT, 6 months
Malus domestica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucitol-6-phosphate
-
696497
Malus domestica
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-glucose 6-phosphate + NADPH + H+
-
696497
Malus domestica
D-glucitol-6-phosphate + NADP+
-
-
-
r
additional information
both, the N- and the C-term tagged enzymes exhibit similar affinity toward substrates
696497
Malus domestica
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
gel filtration, N-term tagged enzyme
Malus domestica
monomer
gel filtration, C-term tagged enzyme
Malus domestica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0011
-
D-glucitol-6-phosphate
C-term tagged enzyme
Malus domestica
0.009
-
D-glucose 6-phosphate
C-term tagged enzyme
Malus domestica
0.222
-
D-glucitol-6-phosphate
N-term tagged enzyme
Malus domestica
0.7
-
D-glucose 6-phosphate
N-term tagged enzyme
Malus domestica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
N- and the C-term tagged enzymes. Both recombinant enzymes similarly show optimal activity in a broad pH range in the direction of D-glucose 6-phosphate reduction, and a narrow range (pH 9.0-10.0) in the opposite oxidative direction
Malus domestica
Cloned(Commentary) (protein specific)
Commentary
Organism
amplified gene cloned into the pGEM-T Easy vector, heterologous expression with a His-tag alternatively placed in the N- or C-terminus in Escherichia coli BL21 Star (DE3) cells
Malus domestica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
-
Malus domestica
KCl
-
Malus domestica
additional information
no significant differences in activity when ATP, ADP, AMP, ADP-glucose, UTP, UDP-glucose, inorganic phosphate or diphosphate (5 mM each), 10% (v/v) glycerol, 500 mM glucitol or 20% (w/v) sucrose are added to the enzyme assay mixture
Malus domestica
NaCl
200 mM cause about 65% inhibition of the enzyme activity
Malus domestica
NH4Cl
-
Malus domestica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
level of soluble recombinant enzyme is much higher in the N-term tagged protein than in the C-term tagged one. In the C-term tagged one the majority of the protein is in the insoluble form
Malus domestica
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34900
-
sequence analysis, A6PRase
Malus domestica
39000
-
gel filtration, C-term tagged enzyme
Malus domestica
68000
-
gel filtration, N-term tagged enzyme
Malus domestica
Purification (Commentary) (protein specific)
Commentary
Organism
one-step protein purification by immobilized metal affinity chromatography, N-term tagged protein 95% pure, C-term tagged one 75% pure
Malus domestica
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Malus domestica
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.017
-
C-term tagged enzyme, purified enzyme
Malus domestica
0.136
-
N-term tagged enzyme, crude extract
Malus domestica
1.25
-
N-term tagged enzyme, 9.2fold purified
Malus domestica
Storage Stability (protein specific)
Storage Stability
Organism
-80░C, 25 mM Tris-HCl buffer, pH 8.0, 300 mM NaCl, 1 mM beta-mercaptoethanol, 10% (v/v) glycerol, 1 mM EDTA, 0.5 mM DTT, 6 months
Malus domestica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucitol-6-phosphate
-
696497
Malus domestica
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-glucose 6-phosphate + NADPH + H+
-
696497
Malus domestica
D-glucitol-6-phosphate + NADP+
-
-
-
r
additional information
both, the N- and the C-term tagged enzymes exhibit similar affinity toward substrates
696497
Malus domestica
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
gel filtration, N-term tagged enzyme
Malus domestica
monomer
gel filtration, C-term tagged enzyme
Malus domestica
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0011
-
D-glucitol-6-phosphate
C-term tagged enzyme
Malus domestica
0.009
-
D-glucose 6-phosphate
C-term tagged enzyme
Malus domestica
0.222
-
D-glucitol-6-phosphate
N-term tagged enzyme
Malus domestica
0.7
-
D-glucose 6-phosphate
N-term tagged enzyme
Malus domestica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
N- and the C-term tagged enzymes. Both recombinant enzymes similarly show optimal activity in a broad pH range in the direction of D-glucose 6-phosphate reduction, and a narrow range (pH 9.0-10.0) in the opposite oxidative direction
Malus domestica
Other publictions for EC 1.1.1.200
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696497
Figueroa
Aldose-6-phosphate reductase f ...
Malus domestica
Biochimie
92
81-88
2010
-
-
1
-
-
-
5
-
1
-
3
-
-
3
-
-
1
-
-
1
3
1
3
2
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
3
-
-
-
-
1
-
1
3
1
3
2
-
-
-
4
-
1
-
-
-
-
-
-
-
-
686514
Li
-
Enzymatic regulation of sorbit ...
Malus domestica
Eur. J. Hort. Sci.
72
12-19
2007
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690167
Li
Photosynthesis in response to ...
Prunus persica
Tree Physiol.
27
1307-1318
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682461
Cheng
Antisense inhibition of sorbit ...
Malus domestica
Planta
220
767-776
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655664
Zhou
Regulation of apple leaf aldos ...
Malus domestica
Funct. Plant Biol.
30
1037-1043
2003
-
-
-
-
-
-
5
4
-
2
2
-
-
1
-
-
1
-
-
1
1
-
2
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
2
-
-
-
-
5
1
4
-
2
2
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347838
Loescher
Sorbitol metabolism and sink-s ...
Malus domestica
Plant Physiol.
70
335-339
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347839
Negm
Characterization and partial p ...
Malus domestica, Prunus armeniaca, Prunus persica, Pyrus communis
Plant Physiol.
67
139-142
1981
-
-
-
-
-
-
6
2
-
-
-
4
-
8
-
-
1
-
-
4
5
1
12
-
2
1
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
6
-
2
-
-
-
4
-
-
-
1
-
4
5
1
12
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-