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show all sequences of 1.1.1.200

Antisense inhibition of sorbitol synthesis leads to up-regulation of starch synthesis without altering CO2 assimilation in apple leaves

Cheng, L.; Zhou, R.; Reidel, E.J.; Sharkey, T.D.; Dandekar, A.M.; Planta 220, 767-776 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
‘Greensleeves’ apple is transformed with a cDNA encoding aldose 6-phosphate reductase in the antisense orientation. Antisense expression of A6PR decreases A6PR activity in mature leaves to approximately 15-30% of the untransformed control. The antisense plants have lower concentrations of sorbitol but higher concentrations of sucrose and starch in mature leaves at both dusk and predawn
Malus domestica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Malus domestica
when sorbitol synthesis is decreased by antisense inhibition of A6PR expression, cytosolic FBPase activity in vivo is down-regulated to reduce the carbon flux to cytosol and starch synthesis in the chloroplast is consequently upregulated
?
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Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Malus domestica
-
Greensleeves
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
when sorbitol synthesis is decreased by antisense inhibition of A6PR expression, cytosolic FBPase activity in vivo is down-regulated to reduce the carbon flux to cytosol and starch synthesis in the chloroplast is consequently upregulated
682461
Malus domestica
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
‘Greensleeves’ apple is transformed with a cDNA encoding aldose 6-phosphate reductase in the antisense orientation. Antisense expression of A6PR decreases A6PR activity in mature leaves to approximately 15-30% of the untransformed control. The antisense plants have lower concentrations of sorbitol but higher concentrations of sucrose and starch in mature leaves at both dusk and predawn
Malus domestica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Malus domestica
when sorbitol synthesis is decreased by antisense inhibition of A6PR expression, cytosolic FBPase activity in vivo is down-regulated to reduce the carbon flux to cytosol and starch synthesis in the chloroplast is consequently upregulated
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
when sorbitol synthesis is decreased by antisense inhibition of A6PR expression, cytosolic FBPase activity in vivo is down-regulated to reduce the carbon flux to cytosol and starch synthesis in the chloroplast is consequently upregulated
682461
Malus domestica
?
-
-
-
-
Other publictions for EC 1.1.1.200
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696497
Figueroa
Aldose-6-phosphate reductase f ...
Malus domestica
Biochimie
92
81-88
2010
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686514
Li
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Enzymatic regulation of sorbit ...
Malus domestica
Eur. J. Hort. Sci.
72
12-19
2007
1
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690167
Li
Photosynthesis in response to ...
Prunus persica
Tree Physiol.
27
1307-1318
2007
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682461
Cheng
Antisense inhibition of sorbit ...
Malus domestica
Planta
220
767-776
2005
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1
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655664
Zhou
Regulation of apple leaf aldos ...
Malus domestica
Funct. Plant Biol.
30
1037-1043
2003
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5
4
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2
2
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347838
Loescher
Sorbitol metabolism and sink-s ...
Malus domestica
Plant Physiol.
70
335-339
1982
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347839
Negm
Characterization and partial p ...
Malus domestica, Prunus armeniaca, Prunus persica, Pyrus communis
Plant Physiol.
67
139-142
1981
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2
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8
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12
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6
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12
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