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show all sequences of 1.1.1.200

Characterization and partial purification of aldose-6-phosphate reductase (alditol-6-phosphate:NADP 1-oxidoreductase) from apple leaves

Negm, F.B.; Loescher, W.H.; Plant Physiol. 67, 139-142 (1981)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
AgNO3
complete inhibition at 0.1 mM
Malus domestica
cysteine
47% inhibition of oxidation and 11% inhibition of reduction at 10 mM
Malus domestica
iodoacetate
42% inhibition of oxidation and 19% inhibition of reduction at 10 mM
Malus domestica
Na2EDTA
35% inhibition of oxidation and 11% inhibition of reduction at 10 mM
Malus domestica
p-chloromercuribenzoate
complete inhibition at 1.0 mM
Malus domestica
ZnSO4
complete inhibition of oxidation and 80% inhibition of reduction at 1.25 mM
Malus domestica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
D-glucose 6-phosphate
-
Malus domestica
3.9
-
D-sorbitol 6-phosphate
-
Malus domestica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Prunus armeniaca
important role in sorbitol synthesis
?
-
-
-
additional information
Prunus persica
important role in sorbitol synthesis
?
-
-
-
additional information
Malus domestica
important role in sorbitol synthesis
?
-
-
-
additional information
Pyrus communis
important role in sorbitol synthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Malus domestica
-
apple, cv. Starkrimson; cv. Antonovka; cv. Golden Delicious
-
Prunus armeniaca
-
apricot, cv. Perfection
-
Prunus persica
-
peach, cv. Redhaven
-
Pyrus communis
-
pear, cv. Conference, cv. Bartlett
-
Purification (Commentary)
Commentary
Organism
-
Malus domestica
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
photosynthetically active tissue
Malus domestica
-
leaf
photosynthetically active tissue
Prunus armeniaca
-
leaf
photosynthetically active tissue
Prunus persica
-
leaf
photosynthetically active tissue
Pyrus communis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
distribution in Rosaceae species; measurement during development
Malus domestica
additional information
-
distribution in Rosaceae species
Prunus armeniaca
additional information
-
distribution in Rosaceae species
Prunus persica
additional information
-
distribution in Rosaceae species
Pyrus communis
628
-
-
Malus domestica
Storage Stability
Storage Stability
Organism
1°C, 1 week, partially purified enzyme
Malus domestica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose 6-phosphate + NADPH
reduced at low rate
347839
Malus domestica
?
-
-
-
?
D-galactose 6-phosphate + NADPH
reduced at a higher rate than D-glucose 6-phosphate
347839
Malus domestica
?
-
-
-
r
D-mannose 6-phosphate + NADPH
reduced at low rate
347839
Malus domestica
?
-
-
-
?
D-sorbitol 6-phosphate + NADP+
-
347839
Prunus armeniaca
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-sorbitol 6-phosphate + NADP+
-
347839
Prunus persica
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-sorbitol 6-phosphate + NADP+
-
347839
Malus domestica
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-sorbitol 6-phosphate + NADP+
-
347839
Pyrus communis
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
additional information
not: D-glucose 1-phosphate, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose, sorbitol
347839
Malus domestica
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Prunus armeniaca
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Prunus persica
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Malus domestica
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Pyrus communis
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
D-sorbitol 6-phosphate oxidation
Malus domestica
35
-
D-glucose 6-phosphate reduction
Malus domestica
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
additional information
-
no activity at 50°C
Malus domestica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
both directions
Malus domestica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8.5
10.1
about 50% of activity maximum at pH 8.5 and 10.1
Malus domestica
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+ or NADH
Malus domestica
NADP+
-
Malus domestica
NADPH
-
Malus domestica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+ or NADH
Malus domestica
NADP+
-
Malus domestica
NADPH
-
Malus domestica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AgNO3
complete inhibition at 0.1 mM
Malus domestica
cysteine
47% inhibition of oxidation and 11% inhibition of reduction at 10 mM
Malus domestica
iodoacetate
42% inhibition of oxidation and 19% inhibition of reduction at 10 mM
Malus domestica
Na2EDTA
35% inhibition of oxidation and 11% inhibition of reduction at 10 mM
Malus domestica
p-chloromercuribenzoate
complete inhibition at 1.0 mM
Malus domestica
ZnSO4
complete inhibition of oxidation and 80% inhibition of reduction at 1.25 mM
Malus domestica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
D-glucose 6-phosphate
-
Malus domestica
3.9
-
D-sorbitol 6-phosphate
-
Malus domestica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Prunus armeniaca
important role in sorbitol synthesis
?
-
-
-
additional information
Prunus persica
important role in sorbitol synthesis
?
-
-
-
additional information
Malus domestica
important role in sorbitol synthesis
?
-
-
-
additional information
Pyrus communis
important role in sorbitol synthesis
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Malus domestica
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
photosynthetically active tissue
Malus domestica
-
leaf
photosynthetically active tissue
Prunus armeniaca
-
leaf
photosynthetically active tissue
Prunus persica
-
leaf
photosynthetically active tissue
Pyrus communis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
distribution in Rosaceae species; measurement during development
Malus domestica
additional information
-
distribution in Rosaceae species
Prunus armeniaca
additional information
-
distribution in Rosaceae species
Prunus persica
additional information
-
distribution in Rosaceae species
Pyrus communis
628
-
-
Malus domestica
Storage Stability (protein specific)
Storage Stability
Organism
1°C, 1 week, partially purified enzyme
Malus domestica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose 6-phosphate + NADPH
reduced at low rate
347839
Malus domestica
?
-
-
-
?
D-galactose 6-phosphate + NADPH
reduced at a higher rate than D-glucose 6-phosphate
347839
Malus domestica
?
-
-
-
r
D-mannose 6-phosphate + NADPH
reduced at low rate
347839
Malus domestica
?
-
-
-
?
D-sorbitol 6-phosphate + NADP+
-
347839
Prunus armeniaca
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-sorbitol 6-phosphate + NADP+
-
347839
Prunus persica
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-sorbitol 6-phosphate + NADP+
-
347839
Malus domestica
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
D-sorbitol 6-phosphate + NADP+
-
347839
Pyrus communis
D-glucose 6-phosphate + NADPH + H+
-
-
-
r
additional information
not: D-glucose 1-phosphate, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose, sorbitol
347839
Malus domestica
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Prunus armeniaca
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Prunus persica
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Malus domestica
?
-
-
-
-
additional information
important role in sorbitol synthesis
347839
Pyrus communis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
D-sorbitol 6-phosphate oxidation
Malus domestica
35
-
D-glucose 6-phosphate reduction
Malus domestica
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
additional information
-
no activity at 50°C
Malus domestica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
both directions
Malus domestica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8.5
10.1
about 50% of activity maximum at pH 8.5 and 10.1
Malus domestica
Other publictions for EC 1.1.1.200
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696497
Figueroa
Aldose-6-phosphate reductase f ...
Malus domestica
Biochimie
92
81-88
2010
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1
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5
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1
-
3
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3
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1
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-
1
3
1
3
2
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-
4
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1
-
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-
1
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-
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5
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-
1
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3
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1
-
1
3
1
3
2
-
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4
-
1
-
-
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-
-
-
-
-
686514
Li
-
Enzymatic regulation of sorbit ...
Malus domestica
Eur. J. Hort. Sci.
72
12-19
2007
1
-
-
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
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-
-
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1
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1
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1
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1
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-
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-
-
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-
690167
Li
Photosynthesis in response to ...
Prunus persica
Tree Physiol.
27
1307-1318
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
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1
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1
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1
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1
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1
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-
-
-
-
-
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-
682461
Cheng
Antisense inhibition of sorbit ...
Malus domestica
Planta
220
767-776
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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1
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1
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1
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-
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-
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655664
Zhou
Regulation of apple leaf aldos ...
Malus domestica
Funct. Plant Biol.
30
1037-1043
2003
-
-
-
-
-
-
5
4
-
2
2
-
-
1
-
-
1
-
-
1
1
-
2
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
2
-
-
-
-
5
1
4
-
2
2
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347838
Loescher
Sorbitol metabolism and sink-s ...
Malus domestica
Plant Physiol.
70
335-339
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
1
-
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-
-
-
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-
-
1
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-
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-
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1
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-
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-
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-
1
3
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
347839
Negm
Characterization and partial p ...
Malus domestica, Prunus armeniaca, Prunus persica, Pyrus communis
Plant Physiol.
67
139-142
1981
-
-
-
-
-
-
6
2
-
-
-
4
-
8
-
-
1
-
-
4
5
1
12
-
2
1
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
6
-
2
-
-
-
4
-
-
-
1
-
4
5
1
12
-
2
1
-
-
1
1
-
-
-
-
-
-
-
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