BRENDA - Enzyme Database show
show all sequences of 1.1.1.20

Enzymatic determination of free glucoronic acid with glucuronolactone reductase. I. isolation and purification of glucoronolactone reductase from rat kidney

Hayashi, S.; Watanabe, M.; Kimura, A.; J. Biochem. 95, 223-232 (1984)

Data extracted from this reference:

General Stability
General Stability
Organism
freezing and thawing: 3 times causes 25% decrease of activity, 5 times causes 40% decrease of activity
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Barbital
-
Rattus norvegicus
Barbiturates
-
Rattus norvegicus
dithiothreitol
-
Rattus norvegicus
Hg2+
-
Rattus norvegicus
iodoacetic acid
-
Rattus norvegicus
NaF
-
Rattus norvegicus
Phenobarbital
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
NADPH
with D-glucuronic acid
Rattus norvegicus
4
-
D-galacturonic acid
-
Rattus norvegicus
6
-
D-glucuronic acid
L-iduronic acid
Rattus norvegicus
9
-
D-glucuronolactone
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000, SDS-PAGE
Rattus norvegicus
41000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-gulono-1,4-lactone + NADP+
Rattus norvegicus
-
D-glucurono-3,6-lactone + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
13450 U/min * mg, 1 unit is defined as change of absorbance of 0.01/min at 340 nm
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-80C, 10 days, 30% loss of activity
Rattus norvegicus
0C, 2 months, less than 10% loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonic acid + NADPH
-
286222
Rattus norvegicus
?
-
-
-
?
D-glucuronic acid + NADPH
-
286222
Rattus norvegicus
L-gulonic acid + NADP+
-
-
-
?
D-glucurono-3,6-lactone + NADPH
oxidation activity is very low
286222
Rattus norvegicus
D-gulono-1,4-lactone + NADP+
-
-
-
r
D-iduronic acid + NADPH
-
286222
Rattus norvegicus
?
-
-
-
?
erythrose + NADPH
-
286222
Rattus norvegicus
glycerol + NADP+
-
-
-
?
glyceraldehyde + NADPH
-
286222
Rattus norvegicus
?
-
-
-
?
L-gulono-1,4-lactone + NADP+
-
286222
Rattus norvegicus
D-glucurono-3,6-lactone + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
180 min, 50% loss of activity; 30 min, 20% loss of activity; 60 min, 30% loss of activity
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7.5
DL-glyceraldehyde
Rattus norvegicus
6
7
D-glucuronic acid
Rattus norvegicus
9.5
-
broad, oxidation of glycerol and L-gulonolactone
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADPH is 145-times as effective as NADH
Rattus norvegicus
NADP+
-
Rattus norvegicus
NADPH
NADPH is 145-times as effective as NADH
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADPH is 145-times as effective as NADH
Rattus norvegicus
NADP+
-
Rattus norvegicus
NADPH
NADPH is 145-times as effective as NADH
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
freezing and thawing: 3 times causes 25% decrease of activity, 5 times causes 40% decrease of activity
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Barbital
-
Rattus norvegicus
Barbiturates
-
Rattus norvegicus
dithiothreitol
-
Rattus norvegicus
Hg2+
-
Rattus norvegicus
iodoacetic acid
-
Rattus norvegicus
NaF
-
Rattus norvegicus
Phenobarbital
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
NADPH
with D-glucuronic acid
Rattus norvegicus
4
-
D-galacturonic acid
-
Rattus norvegicus
6
-
D-glucuronic acid
L-iduronic acid
Rattus norvegicus
9
-
D-glucuronolactone
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000, SDS-PAGE
Rattus norvegicus
41000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-gulono-1,4-lactone + NADP+
Rattus norvegicus
-
D-glucurono-3,6-lactone + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
13450 U/min * mg, 1 unit is defined as change of absorbance of 0.01/min at 340 nm
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-80C, 10 days, 30% loss of activity
Rattus norvegicus
0C, 2 months, less than 10% loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonic acid + NADPH
-
286222
Rattus norvegicus
?
-
-
-
?
D-glucuronic acid + NADPH
-
286222
Rattus norvegicus
L-gulonic acid + NADP+
-
-
-
?
D-glucurono-3,6-lactone + NADPH
oxidation activity is very low
286222
Rattus norvegicus
D-gulono-1,4-lactone + NADP+
-
-
-
r
D-iduronic acid + NADPH
-
286222
Rattus norvegicus
?
-
-
-
?
erythrose + NADPH
-
286222
Rattus norvegicus
glycerol + NADP+
-
-
-
?
glyceraldehyde + NADPH
-
286222
Rattus norvegicus
?
-
-
-
?
L-gulono-1,4-lactone + NADP+
-
286222
Rattus norvegicus
D-glucurono-3,6-lactone + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
180 min, 50% loss of activity; 30 min, 20% loss of activity; 60 min, 30% loss of activity
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7.5
DL-glyceraldehyde
Rattus norvegicus
6
7
D-glucuronic acid
Rattus norvegicus
9.5
-
broad, oxidation of glycerol and L-gulonolactone
Rattus norvegicus
Other publictions for EC 1.1.1.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286223
Nandi
Interrelation of xanthine oxid ...
Rattus norvegicus
Indian J. Exp. Biol.
29
574-578
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286222
Hayashi
Enzymatic determination of fre ...
Rattus norvegicus
J. Biochem.
95
223-232
1984
-
-
-
-
-
1
8
4
1
-
2
1
-
2
-
-
1
-
-
2
1
2
7
1
-
-
1
-
3
-
-
3
-
-
-
-
-
-
3
-
-
1
-
8
-
4
1
-
2
1
-
-
-
1
-
2
1
2
7
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-