Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pectobacterium atrosepticum |
Protein Variants | Comment | Organism |
---|---|---|
D194A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
H198A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
H267A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
H281A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
H363A | 107% of wild-type activity | Pectobacterium atrosepticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.1 mM, 38% residual activity. Activity is completely restored by the addition of 1 mM Co2+ and to 37% by addition of Ni2+, respectively | Pectobacterium atrosepticum | |
NADPH | strong inhibition at high NADPH concentrations, assay below 0.8 mM | Pectobacterium atrosepticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADPH | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
0.4 | - |
pentanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
6.8 | - |
Butanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
- |
Pectobacterium atrosepticum |
42100 | - |
- |
Pectobacterium atrosepticum |
71000 | - |
gel filtration | Pectobacterium atrosepticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium atrosepticum | U6CL97 | - |
- |
Pectobacterium atrosepticum DSM 18077 | U6CL97 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanal + NADPH + H+ | - |
Pectobacterium atrosepticum | butan-1-ol + NADP+ | - |
? | |
butanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM18077 | butan-1-ol + NADP+ | - |
? | |
butanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM 18077 | butan-1-ol + NADP+ | - |
? | |
additional information | enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor | Pectobacterium atrosepticum | ? | - |
? | |
additional information | enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor | Pectobacterium atrosepticum DSM18077 | ? | - |
? | |
additional information | enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor | Pectobacterium atrosepticum DSM 18077 | ? | - |
? | |
pentanal + NADPH + H+ | - |
Pectobacterium atrosepticum | pentan-1-ol + NADP+ | - |
? | |
pentanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM18077 | pentan-1-ol + NADP+ | - |
? | |
pentanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM 18077 | pentan-1-ol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42100, calculated, 2 * 42000, SDS-PAGE | Pectobacterium atrosepticum |
Synonyms | Comment | Organism |
---|---|---|
YqhD | - |
Pectobacterium atrosepticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pectobacterium atrosepticum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | 30-60% of maximum activity | Pectobacterium atrosepticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 40 | stable for at least 30 min | Pectobacterium atrosepticum |
50 | - |
complete inactivation | Pectobacterium atrosepticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.1 | - |
Butanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
49.2 | - |
pentanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pectobacterium atrosepticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pectobacterium atrosepticum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pectobacterium atrosepticum | calculated | - |
5.5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
Butanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
123.1 | - |
pentanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum |