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Literature summary for 1.1.1.2 extracted from

  • Vidal, R.; Lopez-Maury, L.; Guerrero, M.G.; Florencio, F.J.
    Characterization of an alcohol dehydrogenase from the cyanobacterium Synechocystis sp. strain PCC 6803 that responds to environmental stress conditions via the Hik34-Rre1 two-component system (2009), J. Bacteriol., 191, 4383-4391.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.2

Activating Compound

Activating Compound Comment Organism Structure
additional information expression of AdhA is enhanced upon the exposure of cells to different environmental stresses, e.g., hyperosmotic stress (0.5 M sorbitol), salt stress (0.5 M NaCl) or increase in the ambient temperature (45°C). Neither high-light treatment nor oxidative stress (1 mM H2O2) has an influence on the transcription of the adhA gene. Benzyl alcohol also induces adhA gene expression. The induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system. The response regulator Rre1 binds to the promoter region of the adhA gene Synechocystis sp.
additional information the induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system in response to stress conditions Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechocystis sp.
into the pET-28a(+) vector, plasmid pETAdhA expressed in Escherichia coli BL21(DE3) Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information adhA disruption mutant shows no differences in responses to heat shock, salt, or hyperosmotic stress as compared to the wild-type. The adhA mutant strain is unable to grow heterotrophically (added glucose) in darkness, contrary to the wild-type strain Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 cinnamaldehyde
-
 Synechocystis sp.
0.017
-
 cinnamaldehyde in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
0.025
-
 NADPH
-
 Synechocystis sp.
0.025
-
 NADPH in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
0.05
-
 NADP+
-
 Synechocystis sp.
0.05
-
 NADP+ in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
0.075
-
 pentanal
-
 Synechocystis sp.
0.075
-
 pentanal in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
0.11
-
 Butanal
-
 Synechocystis sp.
0.11
-
 Butanal in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
0.27
-
 cinnamyl alcohol
-
 Synechocystis sp.
0.27
-
 cinnamyl alcohol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
0.51
-
 acetaldehyde
-
 Synechocystis sp.
0.51
-
 acetaldehyde in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
1.31
-
 NADH
-
 Synechocystis sp.
1.31
-
 NADH in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
1.54
-
 Pentanol
-
 Synechocystis sp.
1.54
-
 Pentanol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
3.28
-
 butanol
-
 Synechocystis sp.
3.28
-
 butanol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
9.7
-
 NAD+
-
 Synechocystis sp.
9.7
-
 NAD+ in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
18.8
-
 ethanol
-
 Synechocystis sp.
18.8
-
 ethanol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Synechocystis sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Synechocystis sp.
Zn2+
-
 Synechocystis sp.
Zn2+ contains zinc Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36700
-
 3 * 36700, SDS-PAGE Synechocystis sp.
36700
-
 4 * 36700, estimated by SDS-PAGE Synechocystis sp.
140000
-
 gel filtration Synechocystis sp.
140000
-
 SDS-PAGE Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-
Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
by anion-exchange chromatography followed by affinity chromatography on Ni-NTA Synechocystis sp.
DEAE-cellulose resin column chromatography, Ni-NTA column chromatography, and Superose 6 gel filtration Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-propanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 21% relative activity with 1 mM or 84% relative activity with 10 mM substrate Synechocystis sp. propanal + NADPH + H+
-
 r
2-methylbutanol + NADP+ 24% activity with 1 mM 2-methylbutanol, 22% activity with 10 mM 2-methylbutanol Synechocystis sp. 2-methylbutyraldehyde + NADPH + H+
-
 r
2-methylbutanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 24% relative activity with 1 mM or 22% relative activity with 10 mM substrate Synechocystis sp. ? + NADPH + H+
-
 ?
acetaldehyde + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 1% relative activity with 0.1 mM or 23% relative activity with 1 mM substrate Synechocystis sp. ethanol + NADP+
-
 r
benzaldehyde + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 30% relative activity with 0.1 mM or 14% relative activity with 1 mM substrate Synechocystis sp. benzyl alcohol + NADP+
-
 r
benzyl alcohol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 42% relative activity with 1 mM or 79% relative activity with 10 mM substrate Synechocystis sp. benzaldehyde + NADPH + H+
-
 r
benzyl alcohol + NADP+ 42% activity with 1 mM benzyl alcohol, 79% activity with 10 mM benzyl alcohol Synechocystis sp. benzaldehyde + NADPH + H+ 30% activity with 0.1 mM benzaldehyde, 14% activity with 1 mM benzaldehyde r
butanal + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 73% relative activity with 0.1 mM or 120% relative activity with 1 mM substrate Synechocystis sp. butanol + NADP+
-
 r
butanol + NADP+ 23% activity with 1 mM butanol, 70% activity with 10 mM butanol Synechocystis sp. butanal + NADPH + H+ 73% activity with 0.1 mM butanal, 120% activity with 1 mM butanal r
butanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 23% relative activity with 1 mM or 70% relative activity with 10 mM substrate Synechocystis sp. butanal + NADPH + H+
-
 r
cinnamaldehyde + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 61% relative activity with 0.1 mM or 7% relative activity with 1 mM substrate Synechocystis sp. cinnamyl alcohol + NADP+
-
 r
cinnamyl alcohol + NADP+ 100% activity with 1 mM cinnamyl alcohol, 116% activity with 10 mM hexanol Synechocystis sp. cinnamaldehyde + NADPH + H+ 61% activity with 0.1 mM cinnamaldehyde, 7% activity with 1 mM cinnamaldehyde r
cinnamyl alcohol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 100% relative activity with 1 mM or 116% relative activity with 10 mM substrate Synechocystis sp. cinnamaldehyde + NADPH + H+
-
 r
decanal + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 21% relative activity with 0.1 mM or 22% relative activity with 1 mM substrate Synechocystis sp. decanol + NADP+
-
 r
decanol + NADP+ 3% activity with 1 mM decanol, 6% activity with 10 mM decanol Synechocystis sp. decanal + NADPH + H+ 21% activity with 0.1 mM decanal, 22% activity with 1 mM decanal r
decanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 3% relative activity with 1 mM or 6% relative activity with 10 mM substrate Synechocystis sp. decanal + NADPH + H+
-
 r
ethanol + NAD+
-
 Synechocystis sp. acetaldehyde + NADH + H+
-
 r
ethanol + NADP+ 19% activity with 1 mM ethanol, 91% activity with 10 mM ethanol Synechocystis sp. acetaldehyde + NADPH + H+ 1% activity with 0.1 mM acetaldehyde, 23% activity with 1 mM acetaldehyde r
ethanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 19% relative activity with 1 mM or 91% relative activity with 10 mM substrate Synechocystis sp. acetaldehyde + NADPH + H+
-
 r
hexanal + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 53% relative activity with 0.1 mM or 100% relative activity with 1 mM substrate Synechocystis sp. hexanol + NADP+
-
 r
hexanol + NADP+ 27% activity with 1 mM hexanol, 40% activity with 10 mM hexanol Synechocystis sp. hexanal + NADPH + H+ 53% activity with 0.1 mM hexanal, 100% activity with 1 mM hexanal r
hexanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 27% relative activity with 1 mM or 40% relative activity with 10 mM substrate Synechocystis sp. hexanal + NADPH + H+
-
 r
additional information no activity with acetone, 3-pentanone, D-glucose, and 3-pentanol Synechocystis sp. ?
-
 ?
octanal + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 42% relative activity with 0.1 mM or 80% relative activity with 1 mM substrate Synechocystis sp. octanol + NADP+
-
 r
octanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 8% relative activity with 1 mM or 17% relative activity with 10 mM substrate Synechocystis sp. octanal + NADPH + H+
-
 r
octanol + NADP+ 8% activity with 1 mM octanol, 17% activity with 10 mM octanol Synechocystis sp. octanal + NADPH + H+ 42% activity with 0.1 mM octanal, 80% activity with 1 mM octanal r
pentanal + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 100% relative activity with 0.1 mM or 107% relative activity with 1 mM substrate Synechocystis sp. pentanol + NADP+
-
 r
pentanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 31% relative activity with 1 mM or 52% relative activity with 10 mM substrate Synechocystis sp. pentanal + NADPH + H+
-
 r
pentanol + NADP+ 31% activity with 1 mM pentanol, 52% activity with 10 mM pentanol Synechocystis sp. pentanal + NADPH + H+ 100% activity with 0.1 mM pentanal, 107% activity with 1 mM pentanal r
phenylethanol + NADP+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 15% relative activity with 1 mM or 24% relative activity with 10 mM substrate Synechocystis sp. ? + NADPH + H+
-
 ?
phenylethanol + NADP+ 15% activity with 1 mM phenylethanol, 24% activity with 10 mM phenylethanol Synechocystis sp. phenylacetaldehyde + NADPH + H+
-
 r
propanal + NADPH + H+ 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 26% relative activity with 0.1 mM or 87% relative activity with 1 mM substrate Synechocystis sp. 1-propanol + NADP+
-
 r
propanol + NADP+ 21% activity with 1 mM propanol, 84% activity with 10 mM propanol Synechocystis sp. propanal + NADPH + H+ 26% activity with 0.1 mM propanal, 87% activity with 1 mM propanal r

Subunits

Subunits Comment Organism
homotetramer 4 * 36700, estimated by SDS-PAGE Synechocystis sp.
tetramer 3 * 36700, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
AdhA
-
 Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.15
-
 Pentanol
-
 Synechocystis sp.
13.15
-
 Pentanol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
17.05
-
 butanol
-
 Synechocystis sp.
17.05
-
 butanol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
27.35
-
 ethanol
-
 Synechocystis sp.
27.35
-
 ethanol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
60.78
-
 cinnamyl alcohol
-
 Synechocystis sp.
60.78
-
 cinnamyl alcohol in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
107.9
-
 cinnamaldehyde
-
 Synechocystis sp.
107.9
-
 cinnamaldehyde in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
156
-
 Butanal
-
 Synechocystis sp.
156.6
-
 Butanal in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
157.6
-
 acetaldehyde
-
 Synechocystis sp.
157.6
-
 acetaldehyde in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.
159
-
 pentanal
-
 Synechocystis sp.
159.1
-
 pentanal in 30 mM Tris-HCl (pH 8.0) Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Synechocystis sp.
NADH
-
 Synechocystis sp.
NADH the enzyme can use both NADPH and NADH as electron donors, although NADPH is preferred to NADH Synechocystis sp.
NADP+ affinity for NADP+ is higher than that for NAD+ as an alcohol oxidant Synechocystis sp.
NADPH NADPH is preferred to NADH as the reductant for aldehydes Synechocystis sp.
NADPH the enzyme can use both NADPH and NADH as electron donors, although NADPH is preferred to NADH Synechocystis sp.