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Literature summary for 1.1.1.2 extracted from
- A novel NADPH-dependent aldehyde reductase gene from Saccharomyces cerevisiae NRRL Y-12632 involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion (2009), Gene, 446, 1-10.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| protein expression of Y63 using GAL promoter of pYes2NT vector in yeast | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
net Y63 protein | Saccharomyces cerevisiae |
| 45000 | - |
partially purified protein of the histidineY63 complex | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
This UniProt-ID has been deleted; NRRL Y-12632 | - |
| Saccharomyces cerevisiae NRRL Y-12632 | - |
This UniProt-ID has been deleted; NRRL Y-12632 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpressed Y63 protein partially purified by Ni-NTA column chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.27 | - |
with 4-methoxybenzaldehyde as substrate | Saccharomyces cerevisiae |
| 0.58 | - |
with 5-(hydroxymethyl)furan-2-carbaldehyde as substrate | Saccharomyces cerevisiae |
| 0.9 | - |
with acetaldehyde as substrate | Saccharomyces cerevisiae |
| 0.99 | - |
with trans-2-nonenaldehyde as substrate | Saccharomyces cerevisiae |
| 1.28 | - |
with cinnamic aldehyde as substrate | Saccharomyces cerevisiae |
| 1.31 | - |
with benzoic aldehyde as substrate | Saccharomyces cerevisiae |
| 3.63 | - |
with octanaldehyde as substrate | Saccharomyces cerevisiae |
| 3.73 | - |
with heptanaldehyde as substrate | Saccharomyces cerevisiae |
| 3.97 | - |
with phenylacetaldehyde as substrate | Saccharomyces cerevisiae |
| 4.19 | - |
with with furan-2-carbaldehyde as substrate | Saccharomyces cerevisiae |
| 4.99 | - |
with valeraldehyde as substrate | Saccharomyces cerevisiae |
| 6.34 | - |
with butyraldehyde as substrate | Saccharomyces cerevisiae |
| 6.41 | - |
with propionaldehyde as substrate | Saccharomyces cerevisiae |
| 7.67 | - |
with hexanaldehyde as substrate | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methoxybenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | 4-methoxybenzyl alcohol + NADP+ | - |
? |  |
| 4-methoxybenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | 4-methoxybenzyl alcohol + NADP+ | - |
? | |
| 5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | (furan-2,5-diyl)dimethanol + NADP+ | - |
? | |
| 5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | (furan-2,5-diyl)dimethanol + NADP+ | - |
? | |
| acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ethanol + NADP+ | - |
? | |
| acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | ethanol + NADP+ | - |
? | |
| benzoic aldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| butyraldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| cinnamic aldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| furan-2-carbaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| heptanaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| hexanaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| additional information | the enzyme has a conserved catalytic site at Tyr169-X-X-X-Lys173. An indispensable reduction catalytic triad at Ser131, Tyr169, and Lys173, and an approved cofactor-binding motif at Gly11-X-XGly14-X-X-Ala17 near the N-terminus | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | the enzyme has a conserved catalytic site at Tyr169-X-X-X-Lys173. An indispensable reduction catalytic triad at Ser131, Tyr169, and Lys173, and an approved cofactor-binding motif at Gly11-X-XGly14-X-X-Ala17 near the N-terminus | Saccharomyces cerevisiae NRRL Y-12632 | ? | - |
? | |
| octanaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| phenylacetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | 2-phenylethanol + NADP+ | - |
? | |
| phenylacetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | 2-phenylethanol + NADP+ | - |
? | |
| propionaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | propanol + NADP+ | - |
? | |
| trans-2-nonenaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| valeraldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH-dependent aldehyde reductase | - |
Saccharomyces cerevisiae |
| Y63 protein | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | prefered | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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