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Literature summary for 1.1.1.2 extracted from

  • Ernst, M.; Kaup, B.; Mueller, M.; Bringer-Meyer, S.; Sahm, H.
    Enatioselective reduction of carbonyl compounds by whole-cell biotransformation, combining a formate dehydrogenase and (R)-specific alcohol dehydrogenase (2005), Appl. Microbiol. Biotechnol., 66, 629-634.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star, 27°C, Lb-medium, carbenicillin, kanamycin, plasmid pBtacLB-ADH + pBBR1MCS2fdh, 130rpm Levilactobacillus brevis

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
coexpression of formate dehydrogenase and alcohol dehydrogenase reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADH, photometrically at 340 nm in a mixture of 10mM methyl acetoacetate, 250 microM NADH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0 Levilactobacillus brevis
1.3
-
coexpression of formate dehydrogenase and alcohol dehydrogenase, reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADPH, photometrically at 340 nm in a mixture of 10 mM methyl acetoacetate, 250 microM NADPH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0 Levilactobacillus brevis
6.5
-
coexpression of formate dehydrogenase and alcohol dehydrogenase, reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADPH, photometrically at 340 nm in a mixture of 10 mM methyl acetoacetate, 250 microM NADPH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0 Levilactobacillus brevis
8.1
-
cell-free protein Levilactobacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl acetoacetate + NADH + H+
-
Levilactobacillus brevis methyl (R)-3-hydroxy-butanoate + NAD+
-
?
methyl acetoacetate + NADPH + H+
-
Levilactobacillus brevis methyl (R)-3-hydroxy-butanoate + NADP+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay pH Levilactobacillus brevis

Cofactor

Cofactor Comment Organism Structure
NADH maximal activity with 0.7 micromol/min/mg Levilactobacillus brevis
NADPH maximal activity with 6.5 micromol/min/mg Levilactobacillus brevis