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show all sequences of 1.1.1.199

Purification and properties of a new enzyme from Evernia prunastri, which reduces L-usnic acid

Estevez, M.P.; Legaz, E.; Olmeda, L.; Perez, F.J.; Vicente, C.; Z. Naturforsch. C 36, 35-39 (1981)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
thermolabile
Evernia prunastri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
450000
-
gel filtration
Evernia prunastri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-usnic acid + NADH
Evernia prunastri
-
NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Evernia prunastri
-
-
-
Purification (Commentary)
Commentary
Organism
-
Evernia prunastri
Source Tissue
Source Tissue
Commentary
Organism
Textmining
thallus
-
Evernia prunastri
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-usnic acid + NADH
-
389485
Evernia prunastri
NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Evernia prunastri
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
22
37
-
Evernia prunastri
22
-
about 30% of maximal activity
Evernia prunastri
37
-
about 35% of maximal activity
Evernia prunastri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Evernia prunastri
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.8
9.2
-
Evernia prunastri
6.8
-
about 20% of maximal activity
Evernia prunastri
9.2
-
about 50% of maximal activity
Evernia prunastri
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
strict requirement for NADH, not replaceable by NADPH
Evernia prunastri
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
strict requirement for NADH, not replaceable by NADPH
Evernia prunastri
General Stability (protein specific)
General Stability
Organism
thermolabile
Evernia prunastri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
450000
-
gel filtration
Evernia prunastri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-usnic acid + NADH
Evernia prunastri
-
NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Evernia prunastri
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
thallus
-
Evernia prunastri
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-usnic acid + NADH
-
389485
Evernia prunastri
NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Evernia prunastri
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
22
37
-
Evernia prunastri
22
-
about 30% of maximal activity
Evernia prunastri
37
-
about 35% of maximal activity
Evernia prunastri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Evernia prunastri
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.8
9.2
-
Evernia prunastri
6.8
-
about 20% of maximal activity
Evernia prunastri
9.2
-
about 50% of maximal activity
Evernia prunastri
Other publictions for EC 1.1.1.199
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389487
Avalos
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Action of filipin on phytochro ...
Evernia prunastri
J. Plant Physiol.
135
478-482
1989
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1
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1
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1
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389486
Avalos
Equivalence between Pfr and cy ...
Evernia prunastri
Plant Physiol.
84
803-807
1987
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1
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1
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1
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1
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1
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1
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1
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1
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1
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389485
Estevez
-
Purification and properties of ...
Evernia prunastri
Z. Naturforsch. C
36
35-39
1981
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1
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1
1
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1
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1
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1
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1
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1
3
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1
3
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1
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1
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1
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1
1
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1
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1
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1
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1
3
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1
3
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