BRENDA - Enzyme Database show
show all sequences of 1.1.1.198

Molecular cloning and functional characterization of borneol dehydrogenase from the glandular trichomes of Lavandula x intermedia

Sarker, L.S.; Galata, M.; Demissie, Z.A.; Mahmoud, S.S.; Arch. Biochem. Biophys. 528, 163-170 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Lavandula x intermedia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
(+)-borneol
pH 8.0, 32°C
Lavandula x intermedia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27500
-
x * 27500, calculated
Lavandula x intermedia
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lavandula x intermedia
K4N0V2
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
transcripts are specifically expressed in glandular trichomes of mature flowers
Lavandula x intermedia
-
trichome
transcripts are specifically expressed in glandular trichomes of mature flowers
Lavandula x intermedia
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-borneol + NAD+
-
724125
Lavandula x intermedia
(+)-camphor + NADH + H+
camphor is the only product
-
-
ir
additional information
no substrate: 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, perillyl alcohol, and farnesol. The reverse reduction assay in which camphor is used as a substrate and NADH as a cofactor, does not produce detectable amounts of borneol or other products
724125
Lavandula x intermedia
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 27500, calculated
Lavandula x intermedia
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0004
-
(+)-borneol
pH 8.0, 32°C
Lavandula x intermedia
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADP+
Lavandula x intermedia
NAD+
-
Lavandula x intermedia
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Lavandula x intermedia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADP+
Lavandula x intermedia
NAD+
-
Lavandula x intermedia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
(+)-borneol
pH 8.0, 32°C
Lavandula x intermedia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27500
-
x * 27500, calculated
Lavandula x intermedia
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
transcripts are specifically expressed in glandular trichomes of mature flowers
Lavandula x intermedia
-
trichome
transcripts are specifically expressed in glandular trichomes of mature flowers
Lavandula x intermedia
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-borneol + NAD+
-
724125
Lavandula x intermedia
(+)-camphor + NADH + H+
camphor is the only product
-
-
ir
additional information
no substrate: 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, perillyl alcohol, and farnesol. The reverse reduction assay in which camphor is used as a substrate and NADH as a cofactor, does not produce detectable amounts of borneol or other products
724125
Lavandula x intermedia
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 27500, calculated
Lavandula x intermedia
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0004
-
(+)-borneol
pH 8.0, 32°C
Lavandula x intermedia
Other publictions for EC 1.1.1.198
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724125
Sarker
Molecular cloning and function ...
Lavandula x intermedia
Arch. Biochem. Biophys.
528
163-170
2012
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1
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1
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1
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1
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2
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2
1
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1
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2
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1
2
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-
1
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1
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-
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2
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2
1
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1
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-
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726116
Polichuk
A glandular trichome-specific ...
Artemisia annua
Phytochemistry
71
1264-1269
2010
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-
-
-
-
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2
-
-
-
-
-
1
-
-
-
-
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1
6
-
7
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
6
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
286162
Dehal
Metabolism of monoterpenes: Sp ...
Salvia officinalis
Arch. Biochem. Biophys.
258
287-291
1987
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
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-
-
-
-
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-
-
1
1
-
-
-
1
-
2
-
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1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
286163
Croteau
Biosynthesis of monoterpenes. ...
Salvia officinalis
Arch. Biochem. Biophys.
188
182-193
1978
-
-
-
-
-
-
15
3
-
-
1
1
-
1
-
-
1
-
-
1
-
1
12
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
15
-
3
-
-
1
1
-
-
-
1
-
1
-
1
12
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-